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Oil emulsion vaccine and preparation method thereof

一种油乳剂、疫苗的技术，应用在抗病毒剂、医药配方、乳状液输送等方向，能够解决困难等问题，达到减少留存、诱导抗体产生能力强、利于吸收的效果

Active Publication Date: 2012-08-08
PULIKE BIOLOGICAL ENG INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for inactivated vaccines, it is very difficult to prepare a vaccine that can induce sufficient immune protection over a long period of time and has good reproducibility

Method used

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  • Oil emulsion vaccine and preparation method thereof
  • Oil emulsion vaccine and preparation method thereof
  • Oil emulsion vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Newcastle Disease Vaccine by the Method of the Invention and Conventional Methods and Comparative Tests

[0038] 1. Preparation of Newcastle Disease Antigen Oil Emulsion Vaccine by the Method of the Invention

[0039] 1. Dilute the hydrophilic non-ionic surfactant Tween-80 with water in 3 times the volume, then autoclave at 121°C for 30 minutes and cool to room temperature for later use; take a new city with a hemagglutination (HA) titer of 8log2 Epidemic antigen and Tween-80 diluted with 3 times volume of water and sterilized at 121°C for 30 minutes are mixed to form an aqueous phase.

[0040] 2. Mix white oil, propylene glycol, lecithin, and isomannide monooleate as adjuvants, then mix with Span-80 to form the oil phase, autoclave at 121°C for 30 minutes, and cool to room temperature for later use.

[0041] 3. According to the volume ratio of oil phase: water phase = 2:1, first pour the oil phase into the emulsification tank, the stirring speed is 11,...

Embodiment 2

[0071] Example 2 Preparation of avian influenza vaccine by the method of the present invention and conventional methods and comparative test

[0072] 1. Preparation of avian influenza antigen oil emulsion vaccine by the method of the present invention

[0073] 1. Dilute the hydrophilic non-ionic surfactant Tween-80 with water in 3 times the volume, then autoclave at 121°C for 30 minutes and then cool to room temperature for later use; take poultry with a hemagglutination (HA) titer of 9log2 Influenza antigen and Tween-80 diluted with 3 times volume of water and autoclaved at 121°C for 30 minutes are mixed to form an aqueous phase.

[0074] 2. Mix white oil, propylene glycol, lecithin and isomannide monooleate as adjuvants, then mix with Span-80 to form the oil phase, autoclave at 121°C for 30 minutes, and cool to room temperature for later use.

[0075] 3. According to the volume ratio of oil phase: water phase = 2:1, first pour the oil phase into the emulsification tank, the stirring...

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PUM

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Abstract

The invention discloses an oil emulsion vaccine and a preparation method thereof. The oil emulsion vaccine comprises 33.3 volume percent of water phase and 6.7 percent of oil phase, wherein the water phase contains vaccine antigen and hydrophilic nonionic surfactant; and the oil phase contains mineral oil, lecithin, propylene glycol, isomannitol monooleate and oleophylic nonionic surfactant. The vaccine does not contain aluminium stearate, can keep high antibody productivity in a long time and effectively solves the problems of animal stress reaction caused by vaccine injection and vaccine residue.

Description

Technical field [0001] The invention belongs to the technical field of veterinary biological products, and specifically relates to a poultry oil emulsion vaccine and a preparation method thereof. Background technique [0002] my country’s poultry meat products have been closed frequently. From August 1, 1996 to May 24, 2001, the European Union sealed my country’s customs for five years on the grounds that the poultry meat exported from my country contained animal diseases, pesticides, and veterinary drug residues. In 2004, the European Union banned the import of poultry products from my country on the grounds of bird flu. It was not until August 2008 that the EU announced the lifting of the ban on poultry products. Just lifted the ban and encountered the "quota" problem. The prerequisite for solving all problems is to improve the quality of our country's poultry products. In order to reduce the occurrence of poultry diseases, and at the same time, to avoid the negative effects ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K47/44A61K39/12A61K39/145A61K39/17A61K39/295A61P31/14A61P31/16A61K39/39A61K47/10A61K47/24A61K47/26
Inventor 张许科孙进忠乔荣岑
Owner PULIKE BIOLOGICAL ENG INC
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