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Magnolia biondii pamp volatile oil nanoliposome freeze-dried powder, temperature-sensitive magnolia biondii pamp nanogel and preparation method thereof

A nano-liposome and liposome freeze-drying technology, which is applied in the direction of freeze-drying transportation, pharmaceutical formulations, powder transportation, etc., can solve the problems of lack of systematic and in-depth research, easy volatilization of drugs, application restrictions, etc., and facilitate mass preparation And long-term storage, improve drug efficacy, prolong the effect of residence time

Inactive Publication Date: 2010-10-06
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production process of this kind of nasal drops is simple, the curative effect is relatively limited, the drug is volatile, poor stability, low technological content, and lack of systematic and in-depth research, all of which limit the clinical application of magnolia magnolia
[0004] The previous research of the subject group of the present invention has successfully prepared nano-liposomes of magnolia volatile oil, but the storage stability of liquid liposomes is relatively poor
Agglomeration, fusion, and drug leakage are prone to occur during storage, and the main membrane material, phospholipids, is easily oxidized and hydrolyzed, making it difficult to meet the stability requirements of pharmaceutical preparations. These changes have a negative effect on the application of liposomes, which greatly limits the application

Method used

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  • Magnolia biondii pamp volatile oil nanoliposome freeze-dried powder, temperature-sensitive magnolia biondii pamp nanogel and preparation method thereof
  • Magnolia biondii pamp volatile oil nanoliposome freeze-dried powder, temperature-sensitive magnolia biondii pamp nanogel and preparation method thereof
  • Magnolia biondii pamp volatile oil nanoliposome freeze-dried powder, temperature-sensitive magnolia biondii pamp nanogel and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation and performance investigation of nanoliposome freeze-dried powder of magnolia volatile oil

[0054] 1. Instruments and reagents

[0055] 1. Instrument

[0056] Experimental equipment: see Table 1 for details

[0057] Table 1 Experimental Instruments

[0058]

[0059] 2. Reagents

[0060] Experimental raw materials: see Table 2 for details.

[0061] Table 2 Properties and sources of raw materials

[0062]

[0063] 2. Screening of lyoprotectants

[0064] Liquid liposome is a kind of suspension emulsion, which will coagulate, fuse and cause the leakage of the drug contained in it during storage. So freeze-drying protection is very important.

[0065]In order to study the effects of different protective agents and different concentrations of protective agents on the particle size of liposomes during the freeze-drying process, and to find protective agents and their concentrations with better protective effects on liposomes, mannitol, trehalose, and ...

Embodiment 2

[0114] Preparation of temperature-sensitive magnolia nanogel

[0115] For the technical route of preparation of temperature-sensitive magnolia nanogel, see Figure 9

[0116] 1. Preparation of temperature-sensitive dispersion

[0117] Prepare different concentrations of F127 aqueous solution, P188-F127 solution (mixture as shown in Table 4), put it into each centrifuge tube, and put it in an environment of 4°C overnight to fully dissolve it.

[0118] Table 4 P188-F127 ratio

[0119]

[0120] 2. Determination of critical temperature of thermosensitive gel

[0121] (1) Effect of F127 concentration on its own critical temperature

[0122] Fix the centrifuge tube in a constant temperature water tank, let the reagents invade below the liquid surface of the water tank, and raise the temperature with temperature control, 1°C each time, after the temperature stabilizes, last for 10 minutes, then observe whether a gel is formed, and record the temperature at which the gel is for...

Embodiment 3

[0139] In vitro drug release investigation and comparison of temperature-sensitive magnolia nanogel

[0140] 1. In vitro drug release investigation of temperature-sensitive magnolia nanogel

[0141] The drug release in vitro was studied by Franz diffusion cell method. The semi-permeable membrane (molecular weight cut-off 7000u) was fixed between the two pools of the Franz diffusion cell. The area of ​​the Franz diffusion cell used in this experiment was 0.785 cm 2 . Accurately add about 1ml of temperature-sensitive magnolia nanogel into the dosing pool to distribute it evenly on the surface of the semipermeable membrane, and put it in an oven at 33°C for 10 minutes to form a gel. Put the diffusion cell in a 33°C water bath, add 5ml of absolute ethanol that has been preheated to 33°C into the receiving cell as the receiving liquid, put a stirrer in the receiving cell at a speed of 300 rpm, and set the temperature for 5min, 10min, and 20min , 30min, 1h, 2h, 4h, 6h, 8h, 10h, a...

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Abstract

The invention relates to a magnolia biondii pamp volatile oil nanoliposome freeze-dried powder. The magnolia biondii pamp volatile oil nanoliposome freeze-dried powder is prepared by the following steps of: A, preparing a magnolia biondii pamp volatile oil nanoliposome from magnolia biondii pamp volatile oil by a high-pressure homogenization method; and B, preparing the magnolia biondii pamp volatile oil nanoliposome freeze-dried powder from the magnolia biondii pamp volatile oil nanoliposome obtained in the step A by a freeze-drying method, wherein a selected liposome freeze-drying protective agent in the step B is 10 to 15 percent mannitol. The invention further provides a temperature-sensitive magnolia biondii pamp nanogel and a preparation method thereof. In the invention, the magnolia biondii pamp volatile oil nanoliposome is prepared into the freeze-dried powder by the freeze-drying technique creatively so as to improve the stability of a medicament and facilitate large-scale preparation and long-term preservation of the liposome.

Description

【Technical field】 [0001] The invention relates to a liposome freeze-dried powder, in particular to a nano-liposome freeze-dried powder of magnolia volatile oil, temperature-sensitive magnolia nano-gel and a preparation method thereof. 【Background technique】 [0002] Magnolia magnolia is the dry flower bud of Magnoliaceae plant Wangchunhua, Yulan Magnolia or Wudang Magnolia, which has the effect of expelling wind and diverging, and opening the nose. It is mainly used for wind-cold headache, nasal congestion, sinusitis, runny nose, etc. Studies have shown that magnolia volatile oil is one of its active ingredients, which has a wide range of pharmacological activities, such as anti-inflammatory, anti-allergic, anti-histamine and mast cell membrane stabilization, etc., but the volatile oil components in traditional Chinese medicine preparations are volatile and poor in stability, resulting in Uncertainty about its clinical efficacy. [0003] Currently commonly used magnolia na...

Claims

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Application Information

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IPC IPC(8): A61K36/575A61K9/19A61K9/00A61P11/02A61P29/00A61P37/08
Inventor 吴敏王靖马博马碧涛
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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