Chemically synthesized HSV1 virus gD glycoprotein extracellular region gene fragment and expression and application thereof
A technology of chemical synthesis and gene fragments, applied in the fields of vaccines and diagnostic reagents, and genetic engineering technology, can solve problems such as limited clinical effects
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[0106] Detailed description of the embodiment of the present invention:
[0107] Analysis, gene synthesis and vector construction of gD glycoprotein epitope of HSV1 virus
[0108] Analyze the entire amino acid sequence of the gD glycoprotein of the HSV1 virus by computer, screen out strong antigenic epitopes in the gD glycoprotein of the HSV1 virus, and select codons favored by both prokaryotic and eukaryotic, and chemically synthesize the gD glycoprotein of the HSV1 virus containing strong antigens An entirely new gene fragment for the epitope. The gene fragment was cloned into the Hind III / BamH I site in the plasmid pCEP4 to construct the recombinant eukaryotic expression vector pCEP4-gD1.
[0109] Materials and Methods
[0110] 1. Strains and plasmids:
[0111] Escherichia coli DH5α and eukaryotic expression vector pCEP4 are preserved by our laboratory.
[0112] 2. Reagents and instruments:
[0113] PrimerSTAR HS DNA Polymerase, dNTP, Hind III, BamH I, T4 DNA Ligase, P...
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