High-polymer three-dimensional amino-group substrate as well as preparation method and application thereof

A polymer and substrate technology, applied in biochemical equipment and methods, organic compound libraries, combinatorial chemistry, etc., can solve the problems of normal or no signal in the area

Active Publication Date: 2012-07-18
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the silanized amino substrate commonly used in spotting chips has stability problems in spotting high-density chips. In the spotting environment for a long time, the spot diameter is normal at the beginning of spotting, and most of the dot diameters are getting smaller after that. The phe

Method used

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  • High-polymer three-dimensional amino-group substrate as well as preparation method and application thereof
  • High-polymer three-dimensional amino-group substrate as well as preparation method and application thereof
  • High-polymer three-dimensional amino-group substrate as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, by absorbing chitosan on the slide sheet base, prepare chitin substrate

[0043] 1. Pretreatment of glass substrate

[0044] Slide slides in Piranha solution (concentrated H 2 SO 4 / 30%H 2 o 2 , V / V=7:3) soaked overnight, then washed with deionized water, clean; vacuum baked in a vacuum oven at 120 degrees for 1h;

[0045] 2. Adsorption of chitosan polymer

[0046] The molecular weight of chitosan used is 310,000-375,000 Da (high molecular weight);

[0047] Chitosan reaction solution: be the 0.1X PBS damping fluid of the chitosan that final concentration is 0.1% (mass percentage composition), described final concentration is the concentration of chitosan in chitosan reaction solution; Chitosan The pH of the reaction solution was adjusted to 6.0.

[0048] Immerse the clean hydrophobic glass slide base obtained in step 1 into the chitosan reaction solution, soak for 30 hours at 30°C and shake at a speed of 120rpm; stop soaking, wash the slide with dei...

Embodiment 2

[0050] Embodiment 2, by absorbing chitosan on the slide sheet base, prepare chitin substrate

[0051] 1. Pretreatment of glass substrate

[0052] With experiment 1 in embodiment 1;

[0053] 2. Adsorption of chitosan polymer

[0054] The molecular weight of chitosan used is 50,000-190,000 Da (high molecular weight);

[0055] Chitosan reaction solution: be the 0.1X PBS damping fluid of the chitosan that final concentration is 0.3% (mass percentage composition), described final concentration is the concentration of chitosan in chitosan reaction solution; Chitosan The pH of the reaction solution was adjusted to 5.0.

[0056] Immerse the clean hydrophobic glass slide base obtained in step 1 in the chitosan reaction solution, soak for 0.5 hours at 10°C and shake at a speed of 120rpm; stop soaking, wash the slide with deionized water, and dry it. Get the chitin substrate.

Embodiment 3

[0057] Embodiment 3, by absorbing chitosan on the slide sheet base, prepare chitin substrate

[0058] 1. Pretreatment of glass substrate

[0059] With experiment 1 in embodiment 1;

[0060] 2. Adsorption of chitosan polymer

[0061] The molecular weight of chitosan used is 190,000-310,000 Da (high molecular weight);

[0062] Chitosan reaction solution: be the 0.1X PBS damping fluid of the chitosan that final concentration is 0.001% (mass percentage composition), described final concentration is the concentration of chitosan in chitosan reaction solution; Chitosan The pH of the reaction solution was adjusted to 6.0.

[0063] Immerse the clean hydrophobic glass slide base obtained in step 1 in the chitosan reaction solution, soak for 15 hours at 50°C and shake at a speed of 120rpm; stop soaking, wash the slide with deionized water, and dry it. Get the chitin substrate.

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Abstract

The invention discloses a high-polymer three-dimensional amino-group substrate as well as a preparation method and the application thereof. The method for preparing the biochip substrate comprises the following step: connecting amino-enriching polymer on the surface of a slide substrate to obtain a substrate with the amino-enriching polymer being connected on the surface. Experiments prove that the substrate has greatly-improved bimolecular fixing sensitivity, satiated sample points and more stable surface property, and ensures that the shapes of the sample points in the process of long-time sample pointing always keep uniform, and the non-specific adsorption and the self-fluorescence background of the sample points are both very low. The substrate is suitable for the pointing high-density chips which are pointed for a long time, and solves the problem that a major of samples of the high-density chips pointed for a long time can not be fixed in the common amino substrate. The substrate is a biochip substrate material with excellent performance and can be widely applied on the preparation of various biochips.

Description

technical field [0001] The invention relates to a high molecular polymer three-dimensional amino-based sheet and its preparation method and application. Background technique [0002] As an important means of obtaining relevant information efficiently and on a large scale, biochip technology is a synthesis of biotechnology, medical technology, micro-processing technology, microelectronics technology, and material technology, and the application of material technology is concentrated in the biochip substrate ( carrier), that is, the preparation of the substrate. The substrate is the basis of the chip, and the preparation of the substrate is particularly important. [0003] At present, the silanized amino substrate commonly used in spotting chips has stability problems in spotting high-density chips. In the spotting environment for a long time, the spot diameter is normal at the beginning of spotting, and most of the dot diameters are getting smaller after that. The phenomeno...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C03C17/28C03C17/30C40B40/06
Inventor 甘五鹏王素珍吕品王佳黄明贤周玉祥程京
Owner CAPITALBIO CORP
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