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Modified cyanobacteria

A technology of cyanobacteria and bacteria, applied in the field of bacteriology, can solve the problem of low light energy productivity

Inactive Publication Date: 2009-12-30
THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plants because of the year in CO 2 Constraints in dispersal and fixation, growing season, and solar energy harvesting, low productivity in conversion of light energy to biomass and biofuels

Method used

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  • Modified cyanobacteria
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  • Modified cyanobacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0173] Modified Synechocystis PCC 6803 Cyanobacteria for Increased Lipid Content

[0174]Synechocystis PCC 6803, a cyanobacteria, was modified to overexpress the VIPP1 gene sll0617. To achieve high expression levels of the vippl gene (sll0617), it was cloned under the constitutive native promoter of the Synechocystis psbA3 gene (sll1867). The vippl gene was cloned between the upstream (287 bp) and downstream (394 bp) regions of the native psbA3 gene in the spectinomycin analog of the pA31hcgA3 plasmid (He et al., 1999); Figure 14 shown in. The sequence of the vippl (sll0617) gene was obtained from CyanoBase, and two primers were constructed to amplify the 804 bp vippl gene of the Synechocystis genome, 15 bp upstream of the vippl gene start codon, and 18 bp downstream of the vippl gene stop codon. 837bp fragment. The sequences of the primers were 5'-GAG GATAAG TAA GtC ATG aGA TTA TTT GAC and 5'-CTG GCT GAG TTA AtgCAt TTA CAG ATT ATT TAA CC. Lowercase letters indicate nucle...

Embodiment 2

[0186] Dynamic analysis of metabolic flux through the central carbohydrate metabolic pathway of Synechocystis PCC 6803,

[0187] and enhancement of fatty acid biosynthesis by accABCD overexpression

[0188] 1. Materials and methods

[0189] Chemical material. Chemicals used as standards (glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), fructose-1,6-bisphosphate (FBP), glyceraldehyde-3-phosphate (GAP), diphosphate Hydroxyacetone (DHAP), 3-phosphoglycerate (3PG), phosphoenolpyruvate (PEP), 6-phosphogluconate (6PG), ribose-5-phosphate (R5P), ribulose-5-phosphate ( Ru5P), ribulose-1,5-bisphosphate (RuBP) and erythrose-4-phosphate (E4P)) were purchased from (St. Louis, MO). evenly 13 C-labeled D-glucose (U- 13 C 6 -D-glucose) was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA). Milli-Q grade water (Millipore, Van Nuys, CA) was used for all solutions.

[0190] growth conditions and 13 C-glucose label. Synechocystis sp. PCC6803 wild-type and mutants l...

Embodiment 3

[0257] Modified cyanobacteria for increased PHB (bioplastic) content

[0258] 1. Materials and Methods

[0259] Bacterial Strains and Culture Conditions: To understand the physiological role of PHB synthesis in Synechocystis sp. PCC 6803, a series of mutants with altered metabolic pathways resulting in significantly different PHB content were compared under different culture conditions. Deficiency of three terminal oxidases (cytochrome aa 3 mutants of cytochrome c oxidase (CtaI), putative cytochrome bo-type quinol oxidase (CtaII), and cytochrome bd-type quinol oxidase (Cyd)) (Howitt et al., 1998). PS II deletion / oxidase deletion mutants lacking photosynthetic oxygen release and respiratory oxygen consumption were then established by additional deletion of psbB encoding the CP47 protein of photosynthetic system II (Howitt et al., 2001). CyanoRubrum, a mutant strain donated by Dr. Michael Gurevitz (Tel Aviv University, Israel), in which the original cyanobacteria RuBisCO gene ...

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Abstract

Disclosed is a modified photoautotrophic bacterium comprising genes of interest that are modified in terms of their expression and / or coding region sequence, wherein modification of the genes of interest increases production of a desired product in the bacterium relative to the amount of the desired product production in a photoautotrophic bacterium that is not modified with respect to the genes of interest.

Description

[0001] Government Funding Clause Insert [0002] This invention was made with government support under Grant No. DE-FG03-01ER15251 awarded by the US Department of Energy. The government has certain rights in this invention. [0003] CROSS-REFERENCE TO RELATED APPLICATIONS [0004] This application claims priority to US Provisional Patent Application No. 60 / 853,285, filed October 20, 2006, entitled "Modified Cyanobacteria," the entire disclosure of which is hereby incorporated by reference. technical field [0005] The present invention relates generally to the field of bacteriology. In certain aspects, the invention relates to modified photoautotrophic bacteria having a gene of interest overexpressed, downregulated, introduced, deleted, or modified to produce a desired product. The desired products can be processed into biofuels, bioplastics, animal feed additives, nutraceuticals, food additives, fertilizers, and the like. Background technique [0006] The world is cu...

Claims

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Application Information

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IPC IPC(8): C12N1/21A01H13/00C12N15/00C12P7/649
CPCY02E50/13C12P7/6409C12P23/00C12P7/649C12P7/625C12P7/6463Y02E50/10C12N1/20C12N15/00A01H13/00
Inventor 威廉·F·J·弗马斯
Owner THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
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