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Method for producing Ganoderma lucidum mycelium protoplasm

A technology of ganoderma lucidum mycelium and collagen, applied in botany equipment and methods, fertilizer mixture, gardening, etc., can solve the problem of ineffective solution, unstable content of triterpenes and organic germanium, inability to obtain mycelium, etc. problems, to achieve the effect of weakening the effect, avoiding the instability and reduction of effective element content, and wide application range

Inactive Publication Date: 2009-12-30
欧阳小雪
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  • Abstract
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Problems solved by technology

The previous Ganoderma lucidum mycelium products, such as Ganoderma lucidum mycelium capsules and Ganoderma lucidum mycelium powder, were all in accordance with the traditional artificial cultivation method of Ganoderma lucidum, that is, using wood, sawdust or other substitute materials as the cultivation substrate, and through inoculation and cultivation, the Ganoderma lucidum mycelium was grown in Propagate in the culture medium, so the mycelium and the culture medium are mixed together, and the pure mycelium cannot be obtained for human body
[0004] In recent years, the development of modern industrial fermentation production technology has advanced by leaps and bounds. People have begun to study the use of submerged fermentation technology to achieve large-scale production of fungal products, and the mycelium that exists in the fermentation process of Ganoderma lucidum mycelium is liquefied, aged and dead, and the main substance of mycelium is The content of polysaccharides, triterpenes and organic germanium is not stable and has not been effectively resolved, resulting in unsatisfactory efficacy of Ganoderma lucidum mycelium

Method used

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  • Method for producing Ganoderma lucidum mycelium protoplasm

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Materials

[0019] (1) Ganoderma lucidum strain: red lucidum strain

[0020] (2) Raw materials:

[0021] Main raw materials: potato starch, corn flour, soybean meal powder, glucose, yeast extract, xanthan gum, citric acid.

[0022] Growth Factors: Vitamin E, Vitamin B1.

[0023] Mineral elements: potassium dihydrogen phosphate, magnesium sulfate.

[0024] Other substances: soybean oil.

[0025] 2. Main equipment

[0026] Ultra-clean benches, fermentation tanks, shear tanks, colloid mills, homogenizers, filling production lines, autoclaves, negative pressure leak detectors and packaging and labeling equipment

[0027] 3. Medium formula and fermentation conditions

[0028] (1) fermentation medium formula (weight percent)

[0029] Corn flour 1.6-2%, soybean meal 1-1.5%, glucose 1-1.6%, collagen 0.3-0.8%, yeast extract 0.1-0.25%, potassium dihydrogen phosphate 0.1-0.15%, magnesium sulfate 0.03-0.05%, Soybean oil 0.1-0.3%, vitamin E oil 0.05-0.1%, vitamin B1 35-55...

Embodiment 2

[0046] The analysis and research of the present invention proves that the difference in the form of ganoderma products will significantly affect the content of active ingredients. What needs to be emphasized is that although the Ganoderma lucidum products currently on the market, such as Ganoderma lucidum mycelium powder or capsules, contain a certain amount of "active ingredients", their activity is very limited, which is not commensurate with the content of "active ingredients". Tracing it to its cause is the loss of active ingredient activity in the production process. However, the Ganoderma lucidum mycelia puree produced by the production method of the present invention is compared with the existing Ganoderma lucidum products on the market, and the content of "active ingredients" is roughly equivalent (see Table 1), but the content of "active active ingredients" obviously accounts for Excellent (see Table 2), indicating that the ganoderma lucidum mycelia puree produced by ...

Embodiment 3

[0052] Activity experiment of active ingredients in Ganoderma lucidum mycelia puree (observation with naked eyes)

[0053] 1. Divide the experimental mice (the mice with no disease and good nutritional status after inspection) into 6 groups, with 9 mice in each group. in,

[0054] Groups A1 and A2: fed with conventional feed;

[0055] B1 group, B2 group: add the product of embodiment 1 of the present invention in right amount in conventional feed;

[0056] Groups C1 and C2: add Ganoderma lucidum mycelia capsules purchased on the market with the same amount of active ingredients in the conventional feed (remove the capsule shell, and directly mix the contents into the feed).

[0057] Feed until the 20th day and receive radiation exposure at the same time, for 10 consecutive days

[0058] 2. Observation results: the small white mice in group B1 and group B2 who ate the feed added with the product of the present invention still eat normally every day, have slight lethargy, no ...

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Abstract

The invention provides a method for producing Ganoderma lucidum mycelium protoplasm, in particular to a method for producing Ganoderma lucidum mycelium protoplasm by adopting liquid fermentation. Based on the total weight of a liquid medium, the liquid medium comprises the following components by weight percent: 1.6-2.5 percent of corn flour, 1-2 percent of soya bean meal powder, 1-2.5 percent of glucose, 0.3-2 percent of collagen, 0.1-0.5 percent of yeast extract, 0.1-0.5 percent of monopotassium phosphate, 0.01-0.08 percent of magnesium sulfate, 0.1-0.3 percent of soybean oil, 0.05-0.1 percent of vitamin E and 0.5-1.5 percent of potato flour. The method has the characteristics of large cubage, high yield, short production period, small occupied area, stable quality, no pollution and the like, and can be used as an important mode for producing raw materials, such as medicines, health care products or cosmetics and the like.

Description

technical field [0001] The invention relates to a production method of ganoderma mycelium puree, in particular to a method for producing ganoderma lucidum mycelium puree by liquid fermentation. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is listed as the top grade of traditional Chinese medicine in the ancient Chinese medical classics "Shen Nong's Materia Medica". Among them, the mycelium of Ganoderma lucidum is also called "Ganoderma lucidum placenta". Modern scientific research has proved that the mycelium of Ganoderma lucidum contains various active ingredients such as Ganoderma lucidum polysaccharide, Ganoderma lucidum triterpenoids, Ganoderma lucidum organic germanium, and Ganoderma lucidum lectin. The efficacy of Ganoderma lucidum can be summarized into eight aspects: (1) Anti-thrombosis; (2) Enhance hematopoietic function; (3) Improve hyperlipidemia; (4) Prevent arteriosclerosis; (5) Regulate the central nervous system; (6) Improving immune fu...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 欧阳小雪
Owner 欧阳小雪
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