Total triterpene of garden burnet root with anti-inflammatory and analgesic effects and preparation method thereof
An anti-inflammatory, analgesic and total triterpenoid technology, applied in the field of medicine, can solve the problems such as the preparation method of the total triterpenes of Diulum chinensis, which has not yet been seen, and achieve the effects of simple preparation process and clear action mechanism.
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Embodiment 1
[0018] Take 5kg of Burnet coarse powder, add 35L of 70% ethanol to soak overnight, heat and reflux extraction twice, the extraction time is 2 hours and 2 hours respectively, combine the alcohol extracts, filter, and concentrate under reduced pressure at 60°C to reach the crude drug content in the solution. The concentration is 30g / ml, filter; the filtrate is adsorbed by D101 macroporous adsorption resin, the amount of resin is 6kg, the impurities are washed away with water and 20% ethanol respectively, and then eluted with 75% ethanol, the alcohol eluate is collected, and depressurized The solvent was recovered, the obtained crude product was suspended in an aqueous solution, and then subjected to polyamide column chromatography, washed with water first, and then fully eluted with 75% ethanol, and the ethanol was recovered under reduced pressure to obtain 340 g of the total triterpenes.
Embodiment 2
[0020] Take Burnet burnet coarse powder 5kg, add 45L 70% ethanol to soak overnight, heat and reflux extraction 3 times, the extraction time is 2, 2, 1 hour respectively, combine the extracts, filter, and concentrate under reduced pressure at 60°C until the crude drug contained in the solution The concentration is 40g / ml, filter; the filtrate is adsorbed by D101 macroporous adsorption resin, the amount of resin is 6kg, the impurities are washed away with water and 20% ethanol respectively, and then eluted with 70% ethanol solution, the ethanol eluate is collected, decompressed The solvent was recovered, the obtained crude product was suspended in an aqueous solution, and then extracted with equal amounts of ethyl acetate and n-butanol respectively, each extracted 4 times, the solvent was recovered under reduced pressure, and the extracts were combined to obtain 310 g of the total triterpenes.
Embodiment 3
[0021] Example 3 Inhibitory effect of total triterpenes of Burnet burnet on mouse ear swelling induced by xylene
[0022] (1) Experimental materials
[0023] Animals: male KM mice (25±2g), fasted for 24 hours before administration, free to drink water.
[0024] Reagents: normal saline, xylene, 0.5% CMCNa, aspirin (Shenyang Wanrong Pharmaceutical Co., Ltd., product batch number: 061205), indomethacin (Indomethacin) (Shanxi Yunpeng Pharmaceutical Co., Ltd., product batch number: A070801 ).
[0025] Materials and instruments: 1ml mouse gavage device, 1ml syringe, surgical scissors, tweezers, hole punch, volumetric flask, electronic balance (CPJ603 Ohaus Instruments (Shanghai) Co., Ltd.).
[0026] (2) Experimental method
[0027] Mice were randomly divided into 5 groups, 10 in every group: negative control group (0.2% CMCNa, 10ml / kg, i.g.), aspirin control group (30mg / kg -1 , i.g.), indomethacin control group (10mg / kg -1 , i.g.), drug group (100, 30mg / kg, i.g.). Thirty minut...
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