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Phellinus linteus mycelia active glucoprotein and use thereof and preparation

The technology of Phellinus linteus mycelium and glycoprotein is applied in the directions of fungi, glycopeptide components, antibacterial drugs, etc., and achieves the effects of simple production process, high yield and low production cost.

Inactive Publication Date: 2011-01-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above, it is not difficult to find that most of the research on Phellinus Phellinus bioactive substances mainly focuses on the separation and purification of active small molecular compounds or active polysaccharides in its fruiting body or the structure However, there are no reports on the isolation and purification of antibacterial active glycoproteins, identification of physical and chemical properties, and related biological functions

Method used

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  • Phellinus linteus mycelia active glucoprotein and use thereof and preparation
  • Phellinus linteus mycelia active glucoprotein and use thereof and preparation
  • Phellinus linteus mycelia active glucoprotein and use thereof and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046]Phellinus AS3.637 was purchased from China Microorganism Culture Collection Center. The strains were first cultured on slant media. The test tubes and tampons used for culture need to be sterilized at 121°C for 30 minutes, then put into the culture medium and sterilized at 121°C for 30 minutes, and placed on an inclined plane to cool to solidify into a slope. Then the ultra-clean bench was inoculated. The slant culture was carried out at a constant temperature of about 25°C for a period of 7 days. Cut the slant bacteria into small pieces and inoculate them into the seed culture medium. After the medium is divided, put a cotton plug on the Erlenmeyer flask to prevent external microorganisms from entering the medium and cause pollution, and ensure good ventilation performance. . The composition of the seed culture solution is: glucose 20g, yeast extract 5g, corn steep liquor 10g, bran 50g, MgSO 4 ·7H 2 O 0.5g, KH 2 PO 4 1g, the rest is water, and the pH is natural;...

Embodiment 2

[0048] 100g of Phellinus mycelium was added to 1 times the volume of water, and the homogenate was broken, extracted at 4°C, centrifuged, discarded insoluble matter, and the extract was collected. After the extract was concentrated, ammonium sulfate was added to a concentration of 80% (m / V). Stand still for 24 hours, high-speed centrifuge to take the precipitate, add a small amount of water to redissolve the above precipitate, put the precipitate reconstituted solution into an 8000 Dalton dialysis bag, dialyze with deionized water, collect the dialysate, concentrate, and load the sample on DEAE-Sepharose Fast Flow The chromatographic column uses distilled water and NaCl as eluents for stepwise elution, collects the glycoprotein complex components eluted by the NaCl solution, and freezes and dries each collected solution.

[0049] Take each tube and measure the UV absorption at 280nm, and get 4 peaks. After the antibacterial activity test, B and C are the active ingredients, wh...

Embodiment 3

[0051] After adding 3 times the volume of water to 100g Phellinus mycelia, the homogenate was broken, leached at 15°C, centrifuged, discarded the insoluble matter, collected the extract, concentrated the extract and added ammonium sulfate to a concentration of 85% (m / V), Stand still for 36 hours, high-speed centrifuge to take the precipitate, add a small amount of water to redissolve the precipitate, put the precipitate reconstituted solution into an 8000 Dalton dialysis bag, dialyze with deionized water, collect the dialysate, concentrate, and load the sample on the DEAE-Sepharose Fast Flow layer The column was analyzed, and distilled water and NaCl were used as eluents for stepwise elution, and the glycoprotein complex components eluted by the NaCl solution were collected, and each collected solution was freeze-dried.

[0052] Take each tube and measure the UV absorption at 280nm, and get 4 peaks. After the antibacterial activity test, B and C are the active ingredients, whi...

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Abstract

The invention discloses a submerged fermentation phellinus linteus mycelium glycoprotein, a usage and a separation extraction preparation method thereof, the complex is the complex of heteropolysaccharide and protein, wherein, the content of the heteropolysaccharide is 15 to 20 percent, and the heteropolysaccharide is composed of three monosaccharides of glucose monosaccharide, xylose and mannose; the content of the protein is 80 to 85 percent, and the protein is composed of 18 amino acids of aspartic acid, glutamic acid, arginine and so on; and the weight-average molecular weight is 20 to 40KD. The glycoprotein complex uses bran extract liquid as a main ingredient for preparing a culture medium, the phellinus linteus mycelium is produced by the submerged fermentation of the phellinus linteus bacterial strain liquid, the homogenization, the cold-water extraction, the centrifugalization, the collection of supernatant liquid, the precipitation of ammonium sulfate, the dialysis and the DEAE-Sepharose Fast Flow column chromatography are carried out for system separating and purifying the glycoprotein complex. The anti-bacterial glycoprotein is used for preparing the anti-bacterial dugs which have inhibitory effects on escherichia coil and staphylococcus aureus. At the same time, the glycoprotein complex can be used for the separation and the purification of the glycoprotein from the mycelium obtained by the submerged fermentation of various medical and edible fungi.

Description

Technical field: [0001] The invention relates to a Phellinus submerged fermentation mycelia glycoprotein complex with antibacterial and other activities, the use of the complex and a preparation method for separation and purification thereof. Background technique [0002] It is impossible for any kind of organism to exist in isolation in nature. All kinds of organisms can survive because of their own defense mechanisms. Plants and microorganisms can synthesize some small molecular antibiotic substances, such as phenols, tannins and other small molecular substances. At the same time, it can also synthesize antibacterial macromolecules such as polysaccharides, proteins, and glycoprotein complexes. The role of antimicrobial protein is to resist the invasion of antigenic bacteria, improve the disease resistance of the organism itself, and maintain the continuation and normal activities of life activities. The research on antibacterial macromolecules has made great progress in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/14A61K36/06A61P31/04C12N1/14
CPCY02A50/30
Inventor 崔凤杰黄达明罗杰董英张志才邵娜肖香钱静亚
Owner JIANGSU UNIV
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