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Plant pathogenic fungi antagonistic bacteria capable of generating siderophore and uses thereof

A technology of plant pathogenic bacteria and biological inoculum, which is applied in the application field of preventing and controlling plant diseases, and can solve the problems affecting banana quality and export price, pesticide residues, fruit quality effects, etc.

Inactive Publication Date: 2010-06-16
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, we mainly use pesticides such as Miceicarb WP, carbendazim WP, and a small amount of lime-type Bordeaux mixture to directly treat the fruit for control, which has a great impact on the quality of the fruit and brings about the problem of pesticide residues, directly affecting bananas. quality and export price
The use of antagonistic endophytic bacteria to control plant diseases has become a current research hotspot because of its unique characteristics. There have been many reports in this area, but there has been no report on the direct use of banana endophytic bacteria to control banana anthracnose

Method used

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  • Plant pathogenic fungi antagonistic bacteria capable of generating siderophore and uses thereof
  • Plant pathogenic fungi antagonistic bacteria capable of generating siderophore and uses thereof
  • Plant pathogenic fungi antagonistic bacteria capable of generating siderophore and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, screening and identification of phytopathogenic fungus antagonistic bacteria

[0020] 1. Screening of antagonistic bacteria of plant pathogenic fungi

[0021] This strain is isolated from healthy banana plants in Ledong County, Hainan, my country. The specific method is: Weigh 1g of healthy banana plant tissue, after surface disinfection, put it into a sterile mortar with 10mL of sterile water, add a little sterilized Grind the bacterium's quartz sand evenly and let it stand for 15 minutes. Take 1mL and dilute to 10 -3 、10 -4 、10 -5 Concentration gradient, take 0.1mL from each concentration gradient suspension, and spread it on NA medium with a sterile applicator (the formula is: 5g of peptone per liter, 1g of yeast extract, 3g of beef extract, 10g of agar powder, 10g of sucrose , pH 7.0-7.2) on the plate, and the sterile water that washed the sample last time was used as the control. Inverted culture at 28°C, observed continuously for 7 days, picked s...

Embodiment 2

[0045] Example 2, detection of siderophores produced by Acinetobacter baumannii (Acinetobacter baumannii) BEB3 CGMCC No.2244

[0046] Some microorganisms are able to produce a special kind of organic compound that can bind iron, called siderophore. Its main group is a derivative of hydroxamic acid or catechol, which can firmly bind to ferric iron, dissolve ferric iron and transport iron into cells.

[0047] Detect the siderophore ability of Acinetobacter baumannii (Acinetobacter baumannii) BEB3CGMCC No.2244 of the present invention by following experiment

[0048] 1. Preparation of siderophore medium plates (containers and utensils used in the configuration process must be soaked in 6N HCl solution and rinsed with double distilled water):

[0049] 1) First prepare 10×MM9 medium (formula: 3 g of potassium dihydrogen phosphate, 5 g of sodium chloride, 10 g of ammonium chloride, 1000 ml of double distilled water), and autoclave at 121° C. for 20 minutes.

[0050] 2) Add 6ml of ...

Embodiment 3

[0057] Example 3, Antagonism effect experiment of Acinetobacter baumannii (Acinetobacter baumannii) BEB3 CGMCC No.2244 on pathogenic fungi

[0058]Take Acinetobacter baumannii (Acinetobacter baumannii) BEB3CGMCC No.2244 cultured in NB medium (28°C, shaker for 24 hours, OD 600 Value is about 2.5) 2mL into 15mL PDA medium cooled to about 45°C, shake gently to mix the Acinetobacter baumannii (Acinetobacterbaumannii) BEB3 bacteria solution with the medium, and pour into a 9cm diameter petri dish In the middle, cool down and make flat plate, the test pathogenic bacterium bacterium block of diameter 5mm (banana fusarium wilt (purchased from China Agricultural Microorganism Culture Collection Management Center, preservation number ACCC No.31272), banana anthracnose bacterium (purchased from China Agricultural Microbiology Strain Preservation Management Center, preservation number ACCC No.31247), rice blast fungus (purchased from China Agricultural Microorganism Strain Preservation Ma...

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Abstract

The invention discloses a plant pathogenic fungi antagonistic bacterium and the application thereof in the plant disease preventing and curing. The plant pathogenic fungi antagonistic bacterium is acinetobacter baumannii BEB3 CGMCC No.2244. The acinetobacter baumannii BEB3 CGMCC No.2244 of the plant pathogenic fungi antagonistic bacterium can prevent and cure plant diseases caused by banana blightgerms, banana anthracnose germs, rice blast germs, rubber dactylarin wilt disease germs, mango anthracnose germs, and bamboo fusarium germs; in particular can symbiotic to banana, can exist stably inthe banana body, and can prevent and cure banana anthracnose germs and banana blight germs.

Description

technical field [0001] The invention relates to a plant pathogenic fungus antagonist capable of producing siderophore and its application, in particular to a plant pathogenic fungus antagonist capable of producing siderophore and its application in preventing and treating plant diseases. Background technique [0002] Plant endophytic bacteria refer to bacteria that can enter living plant tissues during their lifetime or at least during a certain period of their lifetime, and their existence does not significantly change the phenotypic characteristics and functions of plant tissues. In 1926, Perotti found endophytic bacteria in the root tissues of many healthy plants, and then the research in this area made great progress, and a variety of endophytic bacteria were found in the tissues of many plants. These endophytic bacteria use plant tissue as their habitat, and often promote growth, prevent diseases and insect pests, fix nitrogen, resist adversity (such as drought, etc.), ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 黄贵修刘先宝张科立付业勤蔡吉苗时涛
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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