Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pilose antler basic fibroblast growth factor (DEER FGF) extraction and preparation thereof

A technology of growth factor and growth factor activity, applied in the field of deer antler extract rich in basic fibroblast growth factor, can solve the problem of not providing elution peak map

Inactive Publication Date: 2008-07-09
王利忠
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent CN1104095A discloses a velvet growth factor preparation containing bFGF. After pretreatment, salting out of velvet extract, Sephadex C50 column chromatography and Sepharose affinity column chromatography are required. Change the chromatographic column, and do not provide the spectrum of the elution peak. The so-called active peaks collected only have labels, and the method needs to be explored again to repeat the method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pilose antler basic fibroblast growth factor (DEER FGF) extraction and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, pretreatment of deer antler

[0029] Take 0.5kg of fresh velvet antler, cut the velvet antler into slices with a thickness of 1-2mm with a cryostat, and then add an appropriate amount (200mL) of pre-cooled deionized water to pulverize it with a homogenizer at 4°C, and the homogenization operation is carried out until uniform. End when no tissue mass is visible in the pulp. Then, 200 mL of pre-cooled deionized water was added to the homogenate, and after mixing, ultrasonication was performed under ice bath conditions. The resulting crushed product was centrifuged at 5,000 revolutions per minute (rpm) for 20 minutes, and the supernatant was collected. Add 200 mL of pre-cooled deionized water to the centrifugal pellet, resuspend the pellet and centrifuge again at 5000 rpm for 20 minutes, and take the supernatant. Then, repeat the above process of resuspension and centrifugation once, and take the supernatant. The supernatants obtained by each centrifugal sepa...

Embodiment 2

[0030] Embodiment 2, the chromatographic purification of deer antler basic fibroblast growth factor and the preparation of freeze-dried agent

[0031] 60 mL of the deer antler extract obtained in Example 1 was loaded on a Sephadex G75 gel column (2.6 cm × 110 cm) (purchased from Amersham Company), and then eluted with distilled water at a flow rate of 80 ml / h, and was eluted with an ultraviolet detector. Detection was performed at a wavelength of 280 nm (the elution profile is shown in Figure 1). The products obtained by dialysis of each eluted fraction were detected by the detection method as described in Example 3, and it was found that the peak indicated by "target protein peak" in Figure 1 was deer antler basic fibroblast growth factor. Therefore, the target protein peak was collected, thereby obtaining 55 mL of purified velvet antler basic fibroblast growth factor solution. The obtained deer antler basic fibroblast growth factor solution was put into a dialysis bag and c...

Embodiment 3

[0032] Example 3, detection of basic fibroblast growth factor

[0033] Deer antler basic fibroblast growth factor was detected according to "Guidelines for Molecular Cloning Test" (Science Press, 2002) and MTT colorimetry of Chen Qiongyu et al. (Chinese Journal of Pathophysiology, 2006, 22(2): 247-250). The deer antler basic fibroblast growth factor solution obtained in Example 2 was subjected to isoelectric focusing electrophoresis (using 7.5% polyacrylamide as the supporting medium, using an amphoteric dielectric with pH 3.5 to 10, and focusing at 150V for 4 hours), and after the electrophoresis, the Coomassie brilliant blue staining, the electrophoresis gel photo is shown in Figure 2, there is a single band of basic fibroblast growth factor at about pH 9.5, and the image analysis by the area normalization method shows that its purity reaches 96.8%. Use the proliferation of Schwann cells to detect the biological activity of bFGF: with the blank group added with buffer as a c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method of extracting extract with basic fibroblast growth factor (bFGF) activity from antler, which comprises processing antler maceration extract for one step gel filtration chromatography. In addition, the present invention also relates to antler extract which is prepared by the method and is rich in the basic fibroblast growth factor and the applications of the antler extract at the aspects of medicine, food, cosmetic, etc.

Description

technical field [0001] The invention belongs to the field of medical or edible products of deer antler extract. More specifically, the present invention relates to a method for extracting an extract having basic fibroblast growth factor (bFGF) activity from deer antler and a bFGF-rich deer antler extract prepared by the method. In addition, the present invention also relates to the application of the deer antler extract in medicine, food and cosmetics. Background technique [0002] Deer antler (the young antlers of deer that have not yet been ossified) and antlers are traditional Chinese medicinal materials, which have the functions of strengthening muscles and bones, invigorating kidneys and strengthening yang, and dredging blood vessels. Through modern medicinal chemistry research and analysis, deer antler contains many kinds of physiologically active components, including phospholipids, biogenic amines, prostaglandins, vitamins, amino acids, inorganic salts, unprotected ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/32A61K38/18A61K8/64C07K14/50C07K1/34A61P25/02A61P43/00A61Q19/00A23L1/305
Inventor 朱成钢王利忠
Owner 王利忠
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com