The invention discloses a mycorrhization seedling growing method of the cut root of Moringa Oleifera Lam. The method comprises the steps of seedbed preparation, seed selection and treatment, sand bedaccelerating germination, matrix preparation, bud seedling inoculating and transplanting, and seedling stage management. Mature and full seeds of Moringa Oleifera Lam are selected, and are subjected to sand-storage accelerating germination after being subjected to pest killing and disinfection, after bud seedlings come out, the cut root is inoculated with culture and is then transplanted to a prepared seedling growing container, and high-quality strong seedlings of Moringa Oleifera Lam are cultivated through management and cultivating. By means of the mycorrhization seedling growing method ofthe cut root of Moringa Oleifera Lam, through the procedures of seed treatment, sand-storage accelerating germination, container seedling growing and the like, and by dipping the root with a tailor-made mycorrhization agent before transplanting, the yield of nursery stocks is effectively increased, the quality of the nursery stocks is effectively improved, the disease and pest resistance of the nursery stocks is improved, and the survival rate of the nursery stocks is high, and reaches 95% or above; the root system of the nursery stocks is developed, the nursery stocks can be qualified for selling in advance, the seedlings can be qualified in advance, the seedling growing cost can be lowered, and the mycorrhization seedling growing method has high economic benefits, social benefits and ecological benefits.