The present invention relates to a manhole (10) with a locking device, and more particular to a manhole with a locking device, which comprises a manhole frame (20) having a plurality of insertion slots (24) inwardly protruded from an inner circumferential surface thereof, each insertion slot (24) having a latching protrusion (26) formed in such a manner that a top surface of the insertion slots (24) is partially opened; a bearing member (30) having a female screw portion formed on the center thereof and a plurality of fastening pieces (36) radially formed outwardly from the outer circumferential edge thereof, so that the bearing member (30) is detachably fastened to the manhole frame (20); and a manhole cover (40) having a through-hole (41) centrally formed therein so that an upper portion of the vertical screw rod (42) is rotatably inserted into the through-hole (41), the vertical screw rod (42) having a male screw portion (45) formed on the lower portion thereof so as to be threadably fastened to the female screw portion (35) of the bearing member, a head portion (43) formed on the upper portion thereof so as to abut against an upside surface of the manhole cover (40), and a support portion (44) formed on the upper portion thereof so as to abut against an underside of the manhole cover (40), so that the manhole cover (40) is closed by rotating the head portion (40) of the vertical screw rod (42). Thus, in a case of the manhole (10) mounted on a vehicle road, the manhole cover (40) is prevented from being separated from the manhole frame (20) without permission by a vehicle running at a high speed as well as from frictional noise generated between the manhole cover (40) and the manhole frame (20). Further, in a case of the manhole (10) mounted on the waterside of a river, the manhole (10) maintains its watertightness and is readily opened and closed by an operator.