The invention provides a visual perception-based virtual microscope and its application, belonging to the field of experimental equipment. The virtual microscope based on visual perception includes: a microscope body model and a rotation sensor, a remote communication module, a display, a camera, and an electronic chip arranged on the microscope body model; computing and display equipment; the rotation sensor, a remote communication module, The display and the camera are respectively connected to the electronic chip; the remote communication module can communicate with computing and display equipment. On the one hand, the present invention uses virtual fusion technology to enhance the information of the user's observation results, which is conducive to the user's random exploration of the process, mechanism and principle of the experimental phenomenon; on the other hand, the present invention obtains the operation under real microscope conditions Experience helps experimenters master relevant experimental skills.