The invention discloses a liver positioning puncture device, comprising a horizontal frame, a first motor is arranged on one side of the horizontal frame, the output end of the first motor is fixedly connected with a first shaft coupling, and the first The side of the shaft coupling away from the first motor is fixedly connected with a first screw rod, the surface of the first screw rod is screwed with a first slider, and the bottom of the first slider is fixedly connected with a connection frame, and the A second motor is arranged on one side of the inner wall of the connection frame, the output end of the second motor is fixedly connected with a pinion, the surface of the pinion is engaged with a large gear, and the center of the large gear is fixedly connected with a rotating shaft. The invention relates to the technical field of medical devices. This liver positioning and puncture device solves the problem that the current positioning and puncture of the liver needs to rely on medical personnel to hold the ultrasound probe for positioning and fixing, and then perform anesthesia injection and puncture, which requires the cooperation of multiple people, which is cumbersome and has low positioning accuracy. The problem of low piercing efficiency.