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Trehalulose-containing composition, its preparation and use

a technology of composition and trehalulose, which is applied in the field of trehalulose-containing compositions, can solve the problems of inability to produce compositions containing a high content of isomaltulose, inability to meet the needs of patients, etc., and achieves the effects of improving physical and mental performance, and reducing the risk of diabetes

Inactive Publication Date: 2010-10-21
SUDZUCKER AG MANNHEIM OCHSENFURT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]Thus, the present invention provides the advantageous and unexpected teaching that cells or cell extracts from a microorganism of the genus Pseudomonas and cells or cell extracts from a microorganism of the genus Protaminobacter can be used simultaneously and together in one single process to generate from a sucrose-containing composition a trehalulose-containing composition, preferably with a preselected and easily adaptable composition of its components. Favourably, the present teaching allows to produce a trehalulose-containing composition with a broad range of ratios of its components, particularly trehalulose and isomaltulose. In particular, the combined and simultaneous use of cells or cell extracts from both Pseudomonas and Protaminobacter produces an advantageous composition, in particular sweetener composition, comprising mainly both of trehalulose and isomaltulose, in the following termed “trehalulose-containing composition”, and which provides the advantages detailed below, namely which shows an unexpected glycemic and insulinemic behaviour and opens up further advantageous methods and uses thereof, in particular in controlling body weight and in general glucose and insulin metabolism-related conditions and diabetes. The trehalulose-containing composition of the present invention is essentially non-cariogenic, preferably completely non-cariogenic, and has a low glycemic and low insulinemic index. The present composition is a low calorie composition, which is suitable for health oriented consumers and also in particular for diabetics and combines a pleasant sweetening effect with good bodying properties, which advantageously can be prepared in solid form, but also advantageously can be used in form of a syrup, in particular a suspension or in form of a solution. In particular, the composition of the present invention does not crystallize out in the concentrations used. The compositions of the present invention have—without being bound by theory—a composition, attributed mainly by a specific ratio of it various components, in particular the ratio of trehalulose to isomaltulose and to the minor carbohydrate components isomaltose, isomelezitose and oligomers.DETAILED DESCRIPTION
[0082]In a particularly preferred embodiment the present invention provides a method for reducing the respiratory quotient (RQ=CO2 eliminated / O2 consumed) of a patient, wherein the product or a composition according to the present invention is applied in an amount suitable to achieve the desired effect prior, during or after a physical exercise to the subject in need thereof.

Problems solved by technology

The production of trehalulose-containing compositions which comprise a high content of isomaltulose is for a number of applications, such as jelly or fruit juices, not desirable, since isomaltulose tends to crystallize out, in particular in applications in an aqueous surrounding.
Although the preparation of compositions comprising a high content of trehalulose is for some applications advantageous, it has for other applications the disadvantage of its severely inhibited crystallization capability.

Method used

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  • Trehalulose-containing composition, its preparation and use
  • Trehalulose-containing composition, its preparation and use
  • Trehalulose-containing composition, its preparation and use

Examples

Experimental program
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example 1

Preparation of Trehalulose-Containing Composition

[0091]A. Preparation of the Protaminobacter biocatalyst

[0092]Cells are rinsed off a subculture of the strain Protaminobacter rubrum (CBS 574.77) with 10 ml of a sterile nutrient substrate composed of 8 kg of thick juice from a sugar works (dry matter content 65%), 2 kg of corn steep liquor, 0.1 kg of (NH4)2HPO4 and 89.9 kg of distilled water, adjusted to pH 7.2 if required. This suspension is used as inoculum for the shaker preculture in 1 liter flasks containing 200 ml of nutrient solution of the above composition.

[0093]After an incubation time of 30 hours at 29° C., 18 liters of nutrient solution of the above composition in a 30 liter small fermenter are inoculated with, in each case, 10 flasks (total content 2 liters) and cultivated at 29° C. with 20 liters of air per minute and a stirrer speed of 350 rpm.

[0094]After the organism counts have reached above 5×109 organisms / ml, the cultivation is stopped, the cells are harvested from...

example 2

Sucrose Conversion with Various Ratios of Pseudomonas and Protaminobacter

[0109]In this example, the influence of various ratios of Pseudomonas mesoacidophila MX-45 (FERM BP 3619) and Protaminobacter rubrum CBS 574.77 on the product composition was analysed.

[0110]To prepare the sucrose-containing composition in form of an aqueous medium first solution A was prepared by preparing an aqueous solution of 1.58 g / l calcium acetate (adjusted to a pH of 5.5 with an acetic acid). Then, solution B was prepared by adding solution A up to 1 l to 1.4602 mol / l sucrose corresponding to 500 g sucrose.

[0111]Both strains of the microorganism, that means P. mesacidophila MX-45 and P. rubrum CBS 574.77, were cultivated separately as identified in example 1 A and 1 B. After the organism counts each had reached above 5×109 organism / ml, the cultivation of both microorganisms was stopped, the cells were harvested by centrifugation and pooled together in a 2% strength sodium alginate solution and immobiliz...

example 3

Preparation of Various Jellies

[0118]In the following, four recipes for jellies using as sweeteners a mixture of sucrose and glucose and four recipes using the trehalulose-containing composition of the present invention are prepared. The jellies prepared were tested comparatively in storage and TPA-analysis.

3A: Recipes

[0119]

Sample No.15678Sucrose / Glucose234TrehaluloseTrehaluloseTrehaluloseTrehalulose(240 g drySucrose / GlucoseSucrose / GlucoseSucrose / Glucose(240 g dry(325 g dry(325 g dry(250 g drysubstance,(325 g dry(325 g dry(210 g drysubstance,substance,substance,substance,41.5%)substance 56%)substance, 60%)substance, 24%)41.5%)56%)60%)24%)Pectin 13.2 g 13.2 gH&F, AF 605Citric acid, 5.0 g 6.0 g 5.0 g 7.0 ganhydrousWater290.0 g104.8 g128.8 g 39.3 g232.5 g27.0 g50.9 gSugar120.0 g162.6 g162.6 g105.0 gGlucose150.0 g203.2 g203.2 g131.3 gsyrup DE42 (approx.80% drysubstance)Trehalulose327.5 g443.5 g 443.7 g (73.3% drysubstance)Trehalulose344.7 g(73.1% drysubstance)Lemon 0.4 g 0.4 g 0.4 g 0.5 ...

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Abstract

The present invention relates to a trehalulose-containing composition, its preparation and use.

Description

TECHNICAL FIELD[0001]The present invention relates to a novel trehalulose-containing composition, a process for its preparation and methods for the use thereof.BACKGROUND OF THE INVENTION[0002]Trehalulose (1-O-α-D-glucopyranosyl-D-fructose) and isomaltulose (palatinose, 6-O-α-D-glucopyranosyl-D-fructose) are known structural isomers of sucrose. These isomers are naturally found in honey in small quantities. Both of them are known as non-cariogenic sugars. However, their physical and chemical characteristics are somewhat different, in particular in view of the fact that trehalulose is more soluble in water than isomaltulose with the consequence that their use as food ingredients is different.[0003]Both, isomaltulose and trehalulose, are also known from for instance EP 1 424 074 A1 as being heterodisaccharides, which in comparison to sucrose, show a reduced hydrolysis rate in the small intestine of human or animal consumers, thereby suggesting the provision of nutritional compositions...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/7016A23L1/307A23L1/221A23L1/08C11B5/00A23K1/16A23L2/02A23L1/06A23L2/60A23L1/09C12P19/12A61P3/10A61P3/00A23L2/38A23L19/00A23L21/10A23L21/25A23L27/00A23L27/10A23L33/00A23L33/15A23L33/20
CPCA23L1/2363A23L1/30A23L1/307C12P39/00C12P19/12C12P19/18A61K31/7016A23L27/33A23L33/10A23L33/20A23L33/125A23C9/156A23L2/02A23L2/38A23L2/52C12C5/02A23V2002/00A23V2200/242A23V2200/328A23V2200/3262A23V2200/326A23V2200/332A23V2200/308A23V2200/32A23V2200/30A23V2250/156A23V2250/21A23V2250/606A23V2250/61A23V2250/62A23V2250/636A23V2250/626A23V2250/634A23V2250/64A23V2250/70A23V2250/262A23V2250/06A23V2250/0612A23V2250/1882A23V2250/214A61P1/02A61P1/16A61P19/02A61P3/00A61P3/02A61P3/04A61P35/00A61P3/06A61P5/50A61P9/10A61P3/10A23K20/00
Inventor WACH, WOLFGANGROSE, THOMASKLINGEBERG, MICHAELPETERS, SIEGFRIEDDORR, TILLMANNTHEIS, STEPHANKOWALCZYK, JORGHAUSMANNS, STEPHAN
Owner SUDZUCKER AG MANNHEIM OCHSENFURT
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