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Process for preparing separation of theanine standard contrast product

A preparation process and theanine technology are applied in the field of separation and preparation technology of theanine standard reference substance, which can solve the problems of complex process, high cost, human harm and the like, and achieve safe operation, low production cost and less environmental pollution. Effect

Inactive Publication Date: 2004-09-29
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, there is no theanine standard reference substance available in China; the synthesis of foreign theanine, due to the use of ethylamine or triethylamine toxic and flammable raw materials, is synthesized through high temperature and high pressure, which may cause harm to the human body during production. However, the cost of biochemical synthesis is too high, and these products must be separated and purified by means of solvent distribution and liquid chromatography, and the process is complicated.

Method used

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  • Process for preparing separation of theanine standard contrast product
  • Process for preparing separation of theanine standard contrast product
  • Process for preparing separation of theanine standard contrast product

Examples

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Embodiment

[0015] Example: Take 1 gram of tea extract with a theanine content of 30% (w / w), add 20ml of water to dissolve it, extract it ultrasonically for 20 minutes, centrifuge (11000 rpm), and filter the supernatant with a water membrane , the pore diameter of the water film is 0.45 μm, the concentration of the sample solution is 12 mg / ml, and it is directly prepared by preparative chromatography.

[0016] The chromatographic conditions are:

[0017] Chromatographic column: Prep Nova_pakR HR C18 6μm 19×300mm HPLC column;

[0018] Mobile phase: 0.1% (v / v) formic acid aqueous solution;

[0019] Flow rate: 5ml / min;

[0020] Monitoring detection wavelength: 215nm;

[0021] The injection volume is 1.5ml.

[0022] Prepare the collected solution, concentrate and evaporate to dryness on a water bath to obtain theanine product. The yield is 80%, and the product purity reaches 98.5%; the isocratic preparation chromatogram of theanine is shown in Figure 4 . Theanine ultraviolet scanning ...

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Abstract

The present invention discloses the preparation process of standard theanine contrast product from tea extractive. Tea extractive with theanine content over 10% is prepared into theanine through dissolution in water, ultrasonic extraction, centrifugation to obtain supernatant, water membrane filtering and high efficiency liquid phase chromatographic separation. The quality of the ultimate product is controlled through ultraviolet, infrared and high efficiency liquid phase chromatographic identification. The product has purity reaching 98% (HPLC), and may be used as quantitative and qualitative standard contrast for the high efficiency liquid phase chromatographic measurement of theanine in various tea products.

Description

technical field [0001] The invention relates to a separation and preparation process of theanine standard reference substance. Background technique [0002] Theanine (theanine, N-ethyl-r-L-glutamine) is a unique non-protein amino acid in tea. Modern medical and pharmacological studies have shown that theanine not only has the effect of refreshing, relieving bitterness, and increasing the sweetness of tea soup. , also has the effect of antagonizing the nervous system excitement caused by caffeine and lowering blood pressure. Theanine content is an important indicator of tea quality control. The traditional quantitative analysis methods are mainly strong acid anion exchange resin chromatography, ninhydrin color thin layer chromatography, gas chromatography and derivatization method. The method is cumbersome and has poor anti-interference ability. At present, as the analysis technology becomes more and more mature, there are specialized instruments for the analysis of amino a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C231/24C07C237/06G01N30/04
Inventor 陈波姚守拙朱小兰罗旭彪张斐
Owner HUNAN NORMAL UNIVERSITY
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