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Poplar bark lipoid adjuvant and its preparing method and using method

A technology of poplar bark lipids and adjuvants, which is applied in the direction of medical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problems of no research reports on poplar bark lipids, and achieve protection against sudden degeneration. Active, less irritating, good absorption effect

Inactive Publication Date: 2004-09-29
SHANDONG BINZHOU BOLAIWEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application prospects of poplar bark lipids in medicine are also under continuous in-depth research, but so far there is no research report on poplar bark lipids being used as vaccine adjuvants for livestock and poultry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of poplar bark lipid adjuvants and vaccines:

[0028] The following operations are performed aseptically. 500 grams of lipids crudely extracted from poplar bark (purchased from Shandong Juxian Forestry Chemical Factory) were placed in a water bath at 25°C, and stirred slowly at 100 rpm for 30 minutes. Pass through an 80-mesh copper mesh to obtain a filtrate. The filtrate was then passed through a water bath at 30°C and stirred at a medium speed of 1500 rpm for 20 minutes. After passing through a 50°C water bath and passing through a 120-mesh copper mesh, the filtrate was cooled to room temperature to obtain the poplar bark lipid adjuvant.

[0029] Prepare chicken Newcastle disease virus antigen: get 8 days of SPF chicken embryos (purchased from Jinan SPF chicken farm), sterilize with iodine, and perform aseptic operation, according to each chicken embryo 0.2ml dose will dilute 10000 times of Newcastle disease seed virus (purchased from China Veterinary Dr...

Embodiment 2

[0034] Preparation of poplar bark lipid adjuvants and vaccines:

[0035] The following operations are performed aseptically. Put 1200 g of lipids crudely extracted from poplar bark (purchased from Shandong Ju County Forestry Chemical Factory) in a water bath at 40° C., and slowly stir at 800 rpm for 20 minutes. Pass through a 120-mesh copper mesh to obtain a filtrate. The filtrate was passed through a water bath at 40° C. and stirred at a medium speed of 2500 rpm for 10 minutes. After passing through a 70°C water bath and passing through a 100-mesh copper mesh, the filtrate was cooled to room temperature to obtain the poplar bark lipid adjuvant.

[0036] Prepare chicken egg production drop syndrome virus antigen: get 10 days of SPF duck embryo (purchased from Jinan SPF chicken farm), disinfect with iodine, aseptic operation, dilute 100-fold egg production drop according to each duck embryo 0.2ml dose Syndrome seed poison (purchased from China Veterinary Drug Control Institu...

Embodiment 3

[0039] Immunization of Newcastle Disease Lipid Inactivated Vaccine

[0040] Use the chicken Newcastle disease lipid inactivated vaccine that embodiment 1 makes, immunization program is as follows: 0.3ml / is subcutaneously injected in the neck of 10 days old chicks, immunizes for the second time at the age of 40-60 days, and 0.5ml / is injected neck subcutaneously, Two immunizations can protect the whole growth period from the invasion of chicken Newcastle disease virus. The blood of chickens was collected 14 days after the second immunization, the serum was separated, and the serum antibody was evaluated by the micro-hemagglutination inhibition (HI) method in serology (refer to the "Veterinary Biological Products Regulations" of the Animal Husbandry and Veterinary Bureau of the Ministry of Agriculture). Compared with the immune effect of the same vaccine with propolis as an adjuvant, the immune effect of the lipid vaccine of the embodiment is 2-3 higher than the average antibod...

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Abstract

The present invention relates to immunological adjuvant, and is especially a kind of poplar bark lipoid used as immunological adjuvant and its preparation process and usage. The adjuvant is prepared with coarse extracted lipoid liquid and through stirring, filtering and cooling under certain condition in bacteria-free environment. The adjuvant is easy to maintain and transport without needing special apparatus. The mixture of the adjuvant and antigen in certain ratio is used to immunize animal to produce immunity fast and with less allergy. Immunizing hen with the mixture can also raise laying rate.

Description

technical field [0001] The invention relates to a natural immune adjuvant, in particular to a poplar bark lipid used as an immune adjuvant, and a preparation method and application method of the adjuvant. Background technique [0002] Using modern biotechnology to study vaccines has become the key to solving and preventing many diseases. The emergence of attenuated vaccines has brought surprises to the medical community, but it is far from meeting people's expectations in terms of duration of immunity; inactivated vaccines have the advantages of not spreading live viruses, and will not mutate and become stronger, and are increasingly popular. Therefore, many experts at home and abroad advocate the production and promotion of inactivated vaccines instead of live vaccines. The genetically engineered vaccines that have been studied in the past two decades are designed for the continuous expression of antigens in the body. [0003] Since adjuvants can improve the immunogenicit...

Claims

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Application Information

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IPC IPC(8): A61K39/39
Inventor 张兴晓
Owner SHANDONG BINZHOU BOLAIWEI BIOTECH
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