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Preparing method for heteroossein base materials

A technology of bone collagen and a new method, applied in medical science, prosthesis, etc., can solve the problems of large damage to organic components, influence on mechanical strength, and reduction of mechanical strength, and achieve improved mechanical strength, excellent mechanical properties, and reduced immunogenicity Effect

Inactive Publication Date: 2003-11-19
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These processing methods can remove the antigenic substances of xenograft bone and retain its natural network structure, but the H 2 o 2 The removal of antigenic substances by other strong oxidizing reagents will greatly damage the organic components, which will inevitably affect its mechanical strength, especially the mechanical strength of cancellous bone materials, which is very low. The literature reports bovine cancellous bone by H 2 o 2 After processing, its mechanical strength obviously reduces, handles 24 hours and reduces 43.7%, and its ultimate compressive strength is 5.3564MPa, handles 96 hours and reduces 92.8%, is only 0.6896MPa (Luo Zhuojing, Hu Yunyu, Hou Demen, Zhao Ting

Method used

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  • Preparing method for heteroossein base materials
  • Preparing method for heteroossein base materials
  • Preparing method for heteroossein base materials

Examples

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Effect test

Embodiment 1

[0028] Embodiment 1 A kind of preparation method of bovine cortical bone collagen matrix material

[0029] The cortical bone material of the present invention should be taken from the middle part of the backbone (see attached figure 1). The processing process should be cut with a low-speed saw after freezing to avoid damage to collagen due to high temperature. The processed bone was repeatedly washed with 50°C pressure water, then soaked in 0.1mol / L NaOH solution at room temperature for 24 hours, and the bone was placed in a Soxhlet extractor containing ethyl formate at 60°C for 72 hours, and the bone was degreased every 24 hours. The block was taken out and centrifuged at a speed of 4000 rpm for about 30 min in a centrifuge to remove foreign matter in the bone block; the bone block was partially decalcified in 0.5mol / L HCl solution at room temperature for 10 min, and then dialyzed repeatedly with double distilled water until the pH value was 6. To remove the residual acid s...

Embodiment 2

[0030] Embodiment 2 A kind of preparation method of bovine cancellous bone collagen matrix material

[0031] The cancellous bone material of the present invention should be taken from the epiphysis, and its long axis should be consistent with the arrangement direction of bone trabeculae. The processing process should be cut into blocks with a low-speed saw after freezing to avoid high temperature damage to collagen. The processed bone was repeatedly washed with 50°C pressure water, then soaked in 0.05mol / L NaOH solution at room temperature for 24 hours, and placed in a Soxhlet extractor containing a 1:1 methanol / chloroform mixed solvent at 55-60°C. Degrease for 72 hours, take out the bone pieces every 24 hours, and centrifuge them at a speed of 4000 rpm for about 30 minutes in a centrifuge; partially decalcify the bone pieces in 0.1mol / L HCl solution at room temperature for 10 minutes, and then dialyze them repeatedly with double distilled water to reach the pH value 6, to re...

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Abstract

A process for preparing collagen matrix with heterogeneous bone, used as the bone repairing material and the extracellular matrix of bone tissue engineering, includes such steps as treating ox bone or pig bone, defatting, partial decalcifying, removing non-collagen, proteinas digesting, disinfecting and packing. It has better porosity and pore canal structure.

Description

technical field [0001] The invention relates to the technical field of biomaterials such as bone surgery repair materials and bone tissue engineering extracellular matrix materials. More precisely, the present invention relates to a method for processing heterogeneous cancellous bone and cortical bone by biological and chemical methods to obtain implantable heterogeneous bone repair materials and bone tissue engineering extracellular matrix materials. Background technique [0002] Due to trauma, infection, tumor and dysplasia, the bone loses some bone quality and forms a large gap, which is called bone defect. Due to the large gaps in most bone defects, it is difficult for osteoblasts to crawl through the gaps and normal healing cannot occur, eventually forming nonunions. In the United States, as many as 980,000 cases of bone transplantation are performed for various reasons each year, while in Germany, this figure is as high as 100,000 cases (Kessler S, Wohlfart U M, Ingna...

Claims

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Application Information

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IPC IPC(8): A61L27/24
Inventor 王远亮何创龙杨立华夏烈文
Owner CHONGQING UNIV
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