Method for producing 1,3-propylene glycol through ferment in high cell density by using bacteria in intestinal tract

Abstract

A method of preparing 1, 3- propylene glycol by high cell density intestinal uses the method of fermentation. Its process: seed culturing the intestinal bacterium is activated and then inoculated in the nutrient solution with no bacterium seed; the raises process of fermentation nutrient solution: when this solution is disinfected and cool, we inoculate the seed solution with the volume rate 3-15% into a tank of fermentation solution; the fast grow period of separated fermentation solution: the density of carbon retains 0.1-30g / L; high cell density fermentation period: the density of the bacterium seed reaches OD6502.0-5.0; it adapts the way of limited accommodate of alimentation and air; when the product has been synthesized, we add nitrogen, phosphate, inorganic ion, trace element,VB12 or their mixture of one or several components, and simultaneously add flowing glycerin; when there is acid, the fermentation process finishes.

Description

technical field [0001] The invention relates to a method for preparing 1,3-propanediol through biological fermentation, which belongs to the technical field of biocatalytic synthesis of 1,3-propanediol. Background technique [0002] 1,3-Propanediol is an important raw material and intermediate in organic synthesis and has a wide range of applications. 1,3-propanediol can be used in the synthesis of plasticizers, detergents, preservatives, emulsifiers; it is also used in food, cosmetics and pharmaceutical industries. It can react with isocyanate to produce polyurethane, which is an important monomer for the production of polyurethane; especially 1,3-propanediol can react with terephthalic acid to polymerize into PTT polyester fiber. This new polyester material not only has the characteristics of easy natural circulation Biodegradable, and has excellent resilience, dyeability, stain resistance, etc. It has great achievements in application fields such as carpets, engineering...

AI Technical Summary

Problems solved by technology

When considering how to increase the conversion rate of glycerol in the fermentation process reported in the literature, anaerobic conversion is generally used in the fermentation process. There is a growth stagnation period in anaerobic fermentation, and the growth of bacteria is slow, resulting in low biomass during fermentation and affecting metabolic reactions. Therefore, it is very important to increase the biomass in the fermentation process and adopt high cell density fermentation to increase the production intensity and shorten the production cycle.