Marsh gas engineering inoculum and preparation method thereof
A technology of engineering bacteria and biogas, applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as not being able to meet large-scale energy utilization, project operation efficiency needs to be improved, and negative impact on the reputation of biogas projects , to achieve the effects of shortening hydrolysis and acidification time, improving gas production efficiency and gas production stability, and shortening fermentation start-up time
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Embodiment 1
[0041] Example 1: Take 50kg of biogas engineering biogas slurry with a total solid Ts of 1.8%, and culture it statically at 28°C. First add 1.2% mass fraction of 60-mesh Baiyun Obo iron ore powder, and then add 0.6 mass fraction every 2 days % that is, 0.3kg 60 mesh pig manure until the pH of the culture solution drops to 6.4, and then add a mass fraction of 2.0% every 2 days, that is, 1kg 60 mesh pig manure, until the coenzyme F420 fluorescence spectrophotometry value of the culture solution is 58.2, and then the obtained culture Add 40 meshes of pig manure adjusted to a pH of 7.0 to a moisture content of 50%, the mass ratio of pig manure to culture solution is 1.5:1, and place it at 30°C to dry for 96 hours to obtain a biogas engineering bacterial agent. The bacterial agent was detected by PCR-DGGE, mainly by Dyella marensis , Roseospira goensis , Clostridium lactatifermentans , Clostridium beijerinckii , Kosmotoga olearia , Aminobacterium colombiense , Bacteroi...
Embodiment 2
[0042] Example 2: Take 100kg of urban sewage treatment plant sludge with a total solid Ts of 7%, shake it at 35°C at 60 rpm, add 2.5% mass fraction of 100-mesh Baiyun Obo iron ore powder, and then add mass fraction every other day Fraction 1%, that is, 1kg 60 mesh cow dung until the pH of the culture medium drops to 6.5, and then add a mass fraction of 2% every 3 days, that is 2kg 60 mesh, according to the 1:1 mixture of cow dung and corn stalks until the culture medium coenzyme F420 fluorescence The spectrophotometric value is up to 89.6, and then the obtained culture solution is added with a mixture of 40 meshes of cow dung and peat with a pH of 7.0 and half of the weight until the water content is 60%, and the mass ratio of the mixture to the culture solution is 2.5:1. Dry at 40°C for 60 hours to obtain biogas engineering bacteria agent. The bacterial agent was detected by PCR-DGGE, mainly by Clostridium beijerinckii , Aminobacterium colombiense, Clostridium cellulovora...
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