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Method of extracting phenolic components from chinese medicine red sage root and its freeze dried powder injection agent

An extraction method and salvianolic acid technology, applied in the directions of drug combination, freeze-dried delivery, pharmaceutical formulation, etc., can solve the problems of low activity, low phenolic acid content in the extract, low resin regeneration rate, etc., so as to improve the therapeutic effect and reduce the Side effects, the effect of improving the transfer rate

Inactive Publication Date: 2007-03-21
YANGTZE RIVER PHARM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent, CN1247855A, disclosed in 2000 the method of extracting phenolic acids in salvia miltiorrhiza by resin method after water extraction, because the water extract has many impurities, resulting in low resin regeneration rate and low phenolic acid content in the extract
On this basis, Chinese patent, CN1384090A, disclosed in 2002 the extraction method of improved water extraction and alcohol precipitation and then resin, although the alcohol precipitation process has been added, the loss of phenolic acid caused by alcohol precipitation exceeds 30%, and water The extraction time is long, more than 1 hour, due to the large amount of destruction of phenolic acid caused by heating
Chinese patent, CN1425659A, disclosed a method for extracting salvianolic acid B from salvianolic acid in 2003, which also has the above-mentioned extraction defects, and other active ingredients in salvianolic acid such as salvianolic acid A cannot be separated
At present, there are existing Danshen preparations such as Danshen injection, etc., only the content of Danshensu is relatively high, and the content of phenolic acid is relatively small. There is no formulation of the components of Salvianolic acid, so the activity is relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 is the extraction of phenolic acid components from the traditional Chinese medicine Salvia miltiorrhiza according to the present invention: Take 1 kg of Salvia miltiorrhiza, add 10 L of boiling water at 100° C., and time leaching for 30 minutes while keeping heating. Filter, add 5L of boiling water at 100°C to the filter residue, and time leaching for 30 minutes, during which the heating is maintained. Filter, discard the filter residue, combine the filtrate, and the filtrate is centrifuged in a continuous high-speed centrifuge at a speed of 15,000 rpm. After centrifugation, the supernatant was ultrafiltered with a molecular weight cut-off of 3000. Keep the filtered part, put it on the C8ODS preparative column, load 5mL each time, elute with methanol-water, detect at 270nm, and vacuum-dry the separated components to obtain 32.4mg of component 1 (salvianolic acid B). 2 (salvianolic acid A) 5.6mg, component 3 (protocatechualdehyde) 8.63mg.

Embodiment 2

[0023] Example 2 is another method of extracting phenolic acid components from the traditional Chinese medicine Salvia miltiorrhiza of the present invention: Take 1 kg of Salvia miltiorrhiza, add 12 L of boiling water at 80°C, and time leaching for 30 minutes while keeping heating. Filter, add 6L of boiling water at 80°C to the filter residue, and time leaching for 30 minutes, during which the heating is maintained. Filter, discard the filter residue, combine the filtrate, and the filtrate is centrifuged in a continuous high-speed centrifuge at a speed of 15,000 rpm. After centrifugation, the supernatant was concentrated to 5 L under reduced pressure. Add 5L of ethyl acetate to extract twice, let stand to separate layers, keep the ethyl acetate layer, recover the ethyl acetate under reduced pressure, add a small amount of water to dissolve the residue, put on the C18ODS preparation column, load 5mL each time, wash with methanol-water Desorption, 270nm detection, the separated...

Embodiment 3

[0024] Example 3 Salvianolic acid component freeze-dried powder injection preparation method: Take 20 g of salvianolic acid A, 80 g of salvianolic acid B, 20 g of mannitol, dissolve in 24 L of water, fine filter and freeze-dry to make 1000 sticks of freeze-dried powder injection.

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PUM

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Abstract

A process for extracting danshinolic acids A and B from red sage root includes soaking in hot water, collecting liquid extract, centrifugal separating, ultrafiltering of supernatant, extracting by reverse-phase column or acetate, acidifying, separating organic phase to recover solvent, and separating by reverse-phase column. A freeze dried injection for preventing and treating liver injury and hepafibrosis is prepared from said danshinolic acids A and B and additives. Its advantages are high purity and high output rate.

Description

technical field [0001] The invention relates to a method for extracting phenolic acid components from salvia miltiorrhiza for preventing and treating diseases such as liver injury and liver fibrosis and a freeze-dried powder injection thereof. Background technique [0002] "Natural Product Research and Development" 1998, 11 pages 75-80 reported that the phenolic acid in Salvia miltiorrhiza is the main active ingredient in the treatment of cardiovascular diseases. "Pharmaceutical Bulletin" 1981, 16 pages 24-28 reported that the phenolic acids in salvia miltiorrhiza mainly include salvianolic acid A-C, danshensu, rosmarinic acid, and protocatechualdehyde. Recent studies have shown that phenolic acids have a significant preventive effect on liver damage and liver fibrosis, such as "World Chinese Journal of Digestion" 1999, 7 pages 570-572 report: salvianolic acid A has a good anti-peroxidative liver damage effect; " Chinese Journal of Liver Diseases, 1996, 4, page 86, reported...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/78C07D313/06C07C49/675C07B63/00A61K36/00A61K31/343A61K9/19A61P1/16
Inventor 叶勇王东李旭东戴军平
Owner YANGTZE RIVER PHARM GRP CO LTD
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