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Injectable type collagen-based soft tissue filling material and preparation method thereof

A filling material and soft tissue technology, applied in the field of biomedical materials and its preparation, can solve the problems of poor skin repair effect, poor filling effect, high production cost, etc., to eliminate skin wrinkles and collapse, and be easily tolerated by the human body. The effect of convenient degradation time

Inactive Publication Date: 2007-01-17
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are several problems with this product, including the threat of mad cow disease virus, poor filling effect, continuous multiple injections, and high rebound rate, residual glutaraldehyde is cytotoxic, and its residual amount needs to be strictly controlled. Not yet sold domestically; 2. Human placenta collagen injection developed by Liu Bingci, Chinese Academy of Preventive Sciences
Although this product is relatively safe, it also has immune rejection, and the placental collagen degrades quickly, requires multiple injections and has poor skin repair effect, and the production cost is relatively high; 3. Hafod Bioscience B.V. The composite injection of polymethyl methacrylate (PMMA) microspheres and collagen named "Artecoll", this product uses PMMA, a non-degradable synthetic polymer material, as a filler, and the material remains in the In the body, it has adverse effects on the normal metabolism of the skin, and has the potential to cause skin cancer, which does not conform to people's consumer psychology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for preparing the filling material of the present invention is as follows in sequence:

[0026] 1) Take 1kg of freshly slaughtered pig tendon tissue, wash it, chop it up, soak it in 10 liters of 4.5mol / l NaCl solution for 4 hours, and then soak it in 0.05mol / l tris-hydrochloride (Tris-HCl) (pH=7.5) soak in 10 liters of buffer solution for 4 to 6 hours;

[0027] 2) Soak with a mixture of glucose with a final concentration of 0.5 mol / l and NaCl with a final concentration of 2 mol / l for 2 to 12 hours;

[0028] 3) soak in 0.1mol / l acetic acid solution for 4 to 6 hours, and mash with a tissue masher;

[0029] 4) Soak the slurry again in 5 liters of Tris-HCl (pH=7.5) buffer solution for 4-6 hours;

[0030] 5) Centrifuge, separate the precipitate, dilute the precipitate with distilled water at a mass ratio of 1:50, stir and disperse, adjust the pH value of the system to about 3.0 with pure acetic acid, and add pepsin accounting for 3% of the mass of the precipitat...

Embodiment 2

[0042] The method for preparing the filling material of the present invention is as follows in sequence:

[0043] 1) Take 1 kg of freshly slaughtered calf skin tissue, wash it, chop it, soak it in 10 liters of 10% (mass to volume ratio, g / ml) sodium carbonate solution for 4 hours, then soak it in 4.5mol / l NaCl solution for 4 hours ;

[0044] 2) Soak overnight in glucose solution and 2mol / l NaCl solution with final concentrations of 0.5mol / l respectively;

[0045] 3) soak in 0.1mol / l acetic acid solution for 4 to 6 hours, and mash with a tissue masher;

[0046] 4) Soak the slurry twice in 5 liters of Tris-HCl (pH=7.5) buffer solution for 4-6 hours;

[0047] 5) Centrifuge, separate the precipitate, dilute the precipitate with distilled water at a mass ratio of 1:50, stir and disperse, adjust the pH value of the system to about 3.0 with pure acetic acid, and add pepsin accounting for 3% of the mass of the precipitate at the same time, Stir the reaction at 10°C for 24 hours;

...

Embodiment 3

[0057] The method for preparing the filling material of the present invention is as follows:

[0058] 1) The preparation method of collagen is described in embodiment one or embodiment two;

[0059] 2) Dissolve 0.5 g of collagen sponge in distilled water with pH = 3.0, add 0.1 g of chitosan as a cross-linking agent, and stir and cross-link at room temperature for 1 h;

[0060] 3) The product is freeze-dried again, and then heat-treated at 100-120°C under vacuum for 1 hour to further cross-link and sterilize;

[0061] 4) Add 20ml of sterile phosphate buffer solution with pH=6.8 to the product under sterile conditions, make it fully absorb water and swell, knead into a paste, then add 0.3 heparin, 0.1g dermatan sulfate, cytokine bFGF freeze-dried powder 10 μg, 0.3% lidocaine, mix thoroughly, seal the paste in a disposable syringe under sterile conditions, pack 1-2ml in each tube, and store it below 10°C.

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PUM

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Abstract

The invention discloses an injectable type collagen-based soft tissue filling material and preparation method thereof, wherein the filling material mainly comprises collagen 0.5-20%, heparin 0.1%-10%, cell growth factor 0-1%, cushion liquid 70-99.4%, and its preparation comprises extracting soluble collagen from animal connective tissue, cutting off antigen deciding family from the collagen molecules through proteinase hydrolysis, purifying treatment, crosslinking by utilizing safety and nontoxic crosslinking agent, charging biologically active components, kneading the mixture repeatedly into grease form, sealing under asepsis condition and storing at low temperature.

Description

technical field [0001] The invention relates to a biomedical material and a preparation method thereof, especially a human tissue repair or filling material and a preparation method thereof. Background technique [0002] Tissue engineering technology is a kind of biotechnology proposed and gradually developed in the 1980s. Its basic meaning is to combine cells, synthetic materials or processed natural materials, tissues with cytokines and gene therapy to be widely used in tissue regeneration in vivo or tissue construction in vitro. Therefore, tissue engineering technology is a major revolution in the field of plastic and reconstructive surgery. Tissue-engineered skin products have been approved by the FDA and are manufactured and sold in the United States. At present at home and abroad, research on tissue engineering products has attracted widespread attention. [0003] There are three main categories of similar products currently on the market. One is the products named...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24
Inventor 谢德明
Owner JINAN UNIVERSITY
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