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Method for preparing pharmaceutics of hydrolysate of brain protein

A technology for cerebroprotein hydrolysate and preparation, which is applied in the field of preparation of cerebroprotein hydrolysate preparation, can solve the problems of long production cycle, complicated operation, high price, etc., and achieves reduced production environment requirements, reduced probability of deterioration, and simple and easy operation. row effect

Active Publication Date: 2005-11-23
赛隆药业集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its shortcoming is: (1), equipment investment is big, production cycle is long, and production efficiency is low; Use chromatographic method to purify active ingredient, each process needs 30~48 hours, and production cycle is long; , requires Sephadex G-25 gel column chromatography to be carried out in the 2-8°C cold storage workshop, and the Sephadex G-25 gel chromatography material must be imported, which is expensive; annual production of 1 million (10ml) cerebroprotein hydrolyzate injections Calculated by liquid, the above-mentioned investment is more than 2 million yuan; the steps of vacuum concentration and centrifugation are used, the production efficiency is low, and it is not conducive to the control of drug quality
(2), use a large amount of organic chemical solvents, acetone and ether have extremely low boiling points, are flammable, explosive, poisonous dangerous chemicals, ether is also used as an anesthetic, use a large amount of acetone and ether to precipitate brain protein first It is made into brain powder. This step is very dangerous and must be produced in an explosion-proof workshop. The operation is cumbersome, pollutes the environment, and endangers the health of production operators.
Its shortcoming is: (1) use ultrafiltration membrane to carry out ultrafiltration concentration recovery rate is low: although this process has adopted ultrafiltration technology, what use is that the ultrafiltration membrane that cut-off molecular weight is 1000 is concentrated, and effective active ingredient brain polypeptide is different The degree of loss, that is, loss in the permeate, greatly reduces the yield
(2) Multiple passes through microporous membranes with a pore size of 0.5-1 μm and ultrafiltration membranes with a cut-off molecular weight of 70,000 and 10,000 to obtain ultrafiltrate, which makes the operation more cumbersome

Method used

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  • Method for preparing pharmaceutics of hydrolysate of brain protein
  • Method for preparing pharmaceutics of hydrolysate of brain protein
  • Method for preparing pharmaceutics of hydrolysate of brain protein

Examples

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Embodiment 1

[0017] 1. Take 100 kg of fresh pig brain, remove the capsule and blood vessels on the surface, add 100L of purified water (hereinafter referred to as water) while homogenizing, heat to 85°C with water steam, 15 minutes, and quickly cool down to 40°C 6mol / L hydrochloric acid to adjust the pH to 1.5, add 3Kg pepsin for enzymolysis for 3 hours, adjust the pH to 8.0 with 12mol / L sodium hydroxide, add 2Kg trypsin multienzyme (trypsin, pancreatic lipase and pancreatic starch enzyme), add 0.04mol / L CaCl before use 2 Activate trypsin multienzyme for 1 hour, enzymatically hydrolyze for 2 hours at 42°C, then adjust the pH value to 3.0 with 6mol / L hydrochloric acid, and freeze at -20°C for 12 hours; Filter through gauze, add sodium hydroxide to adjust the pH value to neutral, filter and clarify with a microporous membrane with a pore size of 1.0 μm, take the clarified liquid, and ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 10,000 to collect the permeat...

Embodiment 2

[0019] Take 100 kg of fresh pig brain, remove the capsule and blood vessels on the surface, add 200L of purified water (hereinafter referred to as water) while homogenizing, heat to 80°C with water steam for 15 minutes, and quickly cool down to 45°C, Use 6mol / L hydrochloric acid to adjust the pH to 2.0, add 3Kg pepsin for enzymatic hydrolysis for 12 hours, then use 12mol / L sodium hydroxide to adjust the pH to 7.7, add 2Kg trypsinase, and add 0.04mol / L CaCl before use 2Activate trypsin multienzyme for 0.5 hours, enzymatically hydrolyze for 4 hours at 38°C, then adjust the pH value to 2.0 with 6mol / L hydrochloric acid, and freeze at -10°C for 48 hours; at room temperature, use multi-layer Filter through gauze, add sodium hydroxide to adjust the pH value to neutral, filter and clarify with a microporous membrane with a pore size of 0.2 μm, take the clarified liquid, and ultrafilter with an ultrafiltration membrane with a molecular weight cut-off of 10,000 to collect the permeate, ...

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Abstract

A hydrate of brain protein is prepared from pig's brain through adding purified water, homogenizing, heating, cooling, regulating pH=1.5-2.0, enzymolyzing, regulating pH=7.7-8.0, enzymolyzing, regulating pH=2.5-3.0 freezing, thawing, filter, regulating pH to become neutral, ultrafiltering, concentrating, sterilizing, adding amino acids, regulating peptide map, diluting, and steam sterilizing.

Description

technical field [0001] The invention relates to a preparation method of medicine, in particular to a preparation method of cerebroprotein hydrolyzate preparation. Background technique [0002] Cerebroprotein hydrolyzate, as an active brain polypeptide substance extracted from healthy and fresh animal brain tissue by multi-enzyme hydrolysis, contains a variety of essential amino acids for human brain and important elements such as cephalin, lecithin, and peptide nerve growth factor. It acts on the central nervous system in various ways, regulates and improves the metabolism of neurons, promotes the formation of synapses, induces the differentiation of neurons, and further protects nerve cells from various ischemia and neurotoxin damage. It can pass through the blood-brain barrier, promote protein synthesis in the brain, affect the respiratory chain, protect against hypoxia, and improve energy metabolism in the brain. Activates adenylyl cyclase to catalyze other hormone syste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K35/30A61K38/01A61P9/10A61P25/00
Inventor 严家定
Owner 赛隆药业集团股份有限公司
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