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Preparation method and application of poplar transformed product for improving enteritis

A conversion product, poplar technology, which is applied in the field of poplar conversion product preparation, can solve problems such as the lack of core technology for deep processing, and achieve the effect of cheap raw material sources, abundant raw material sources, and mild reaction conditions

Pending Publication Date: 2022-08-02
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on poplar waste lacks both development and utilization oriented to clear active functions, and also lacks effective core technology for deep processing

Method used

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  • Preparation method and application of poplar transformed product for improving enteritis
  • Preparation method and application of poplar transformed product for improving enteritis
  • Preparation method and application of poplar transformed product for improving enteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Using poplar processing waste poplar sawdust (PM) and poplar leaves (PL) as raw materials, drying the poplar sawdust and poplar leaves at 60° C. for 8 hours, pulverizing them through a 200-mesh sieve respectively, and collecting poplar sawdust powder and poplar leaves pink;

[0034] (2) Using 90% ethanol solution as a solvent, the poplar sawdust powder and poplar leaf powder were extracted under heat reflux at 60°C, the ratio of solid to liquid was 1:5, the extraction time was 2.5h, and the extraction was performed 3 times, followed by suction filtration and decompression. Evaporation to obtain an extract-like substance;

[0035] (3) The water-soluble extract material is extracted with petroleum ether and ethyl acetate in turn. The extraction time of petroleum ether is 4h, and the extraction is performed 3 times. The remaining solution is extracted with ethyl acetate, and the extraction time is 4h, and the extraction is performed 3 times. Collect, evaporate under r...

Embodiment 2

[0040] (1) Select 11 kinds of enzymes produced in the laboratory: xylosidase Xin-Dt from D. thermophilum (optimum temperature 75°C, optimum pH 6.0) and xylosidase TtGH39 from T. thermosaccharolyticum (optimum pH 6.0) Optimum temperature 50℃, optimum pH 5.5), α-rhamnosidase AMIHA from A. mulundensis (optimum temperature 65℃, optimum pH 6.0) and α-rhamnoside from D.thermophilum DSM3960 Enzyme DthRha (optimum temperature 90°C, optimum pH 6.5), and 7 kinds of β-glucosidases, respectively Dth3 (optimum temperature 85°C, optimum pH 5.5) derived from D. thermophilum DSM3960, source Tpebgl1 from T.petrophlia DSM 13995 (optimum temperature 90°C, optimum pH 6.0), Sisbgl1 from S. islandicus (optimum temperature 90°C, optimum pH 5.5), Tthbgl1 from T.thermarum DSM5069T ( Optimum temperature 90℃, optimum pH 4.8), Tthbgl3 derived from T.thermarum DSM5069T (optimum temperature 90℃, optimum pH 5.0), Tpebgl3 derived from T.petrophlia DSM 13995 (optimum temperature 90℃, optimum pH 5.0) Optimum ...

Embodiment 3

[0045] Take 20 mg of the ethyl acetate extraction phase (EAE) of poplar sawdust and poplar leaves in Example 1, dissolve in 155 μl of distilled water, add 50 μl of pH 5.5 phosphate buffer and 245 μl of β-glucosidase Dth3 (enzymatic activity 40U / ml), respectively. The poplar extracts were catalyzed by water bath reaction at 85°C, 90°C, and 95°C for 30 min, and the catalyzed products were collected.

[0046] The composition and content of the extract before and after the catalytic reaction were compared by HPLC. The experimental results are as image 3 shown.

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Abstract

The invention discloses a preparation method and application of a poplar transformed product for improving enteritis. The poplar transformed product is prepared by hydrolyzing a poplar extract with beta-glucosidase. Wherein the raw material of the poplar extract is poplar sawdust and / or poplar leaves abandoned in poplar processing, and the beta-glucosidase is prepared through microbial fermentation or recombinant bacterium expression and purification. The preparation method provided by the invention has the characteristics of abundant raw material resources, low cost, simplicity, environmental protection, mild reaction, good anti-inflammatory activity of the extract and the like, and the in-vitro anti-inflammatory activity is respectively improved by 30.39% and 23.98% compared with the poplar sawdust extract and the poplar leaf extract which are obtained without enzyme hydrolysis. The extract prepared through the technology has the effect of improving animal colitis, and comprehensive utilization of poplar resources can be promoted.

Description

technical field [0001] The invention belongs to the technical field of natural substance extraction, and in particular relates to a preparation method and application of a poplar transformation product for improving enteritis. Background technique [0002] Poplar is widely distributed in eastern China and is one of the important afforestation and wood processing tree species in my country. Poplar bark, branches and leaves are rich in phenolic glycosides, flavonoids, organic acids, tannins and other plant secondary metabolites, which can play a certain role in anti-inflammatory, antibacterial, antioxidant and other aspects. As a series of known glycosylated and esterified derivatives of salicyl alcohol, salicylic glycosides have excellent biological activities. Poplar bark has antibacterial effects against Shigella, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and the bark of Poplar trembling can also have good antibacterial effects on Botrytis cinerea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/76A61P1/00A23K10/32A23K20/10A61K127/00A61K135/00
CPCA61K36/76A61P1/00A23K10/32A23K20/10A61K2236/19A61K2236/333A61K2236/35A61K2236/39Y02E50/10
Inventor 房仙颖王文杰赵林果
Owner NANJING FORESTRY UNIV
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