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Kefir whey and tea polyphenol composition as well as preparation method and application thereof

A technology of kefir whey and tea polyphenols, which is applied in the field of kefir whey and tea polyphenols composition and its preparation, can solve the problems of general immunosuppressive effect, different therapeutic effects, and many side effects, etc. Achieve the effect of restoring the diversity and richness of flora, improving the imbalance of intestinal flora, and improving the symptoms of colitis

Pending Publication Date: 2022-07-22
SOUTH CHINA AGRI UNIV
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  • Claims
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Problems solved by technology

[0004] The first category is western medicine. According to the different mechanism of action, western medicine is divided into 5 types. 1) Aminosalicylic acid drugs have good curative effect and low price, but they have many adverse reactions, such as nausea, headache, etc. , and due to long-term drug resistance, dose-dependent
2) Glucocorticoid drugs, which have strong anti-inflammatory and immune-suppressing effects, can be used for the treatment of inflammatory bowel disease, but they have many side effects, which are easy to cause osteoporosis, hyperglycemia, insulin resistance, fat Deposition disorders, hypertension, muscle atrophy and many other diseases
However, the immunosuppressive effect of these drugs is average, and they can only be used as adjuvant therapy in clinical practice, and the side effects are extremely severe, including bone marrow suppression (granulocytopenia), and are highly toxic to the liver and kidney.
4) Biological agents can directly act on targets such as tumor necrosis factor α and cell adhesion molecules, and can treat severe UC patients who have failed traditional treatments, but the price is very high and there are many side effects, such as various decrease in vital cells
5) Anti-infective drugs, for patients with severe UC and UC caused by bacterial infection, antibiotics are often used to heal the fistula of UC patients faster and prevent recurrence, but long-term use will lead to drug resistance and side effects
The second category is traditional Chinese medicine. Traditional Chinese medicine is extensive and profound. Through dialectical treatment, in addition to internal treatment with oral medication, it also cooperates with external treatment such as enema and application, which has a good therapeutic effect on inflammatory bowel disease. The inconsistency between dialectics and clinical treatment and other deficiencies, the treatment effect varies from person to person
Although probiotic preparations can improve and relieve UC symptoms by regulating the intestinal flora, the effect is unstable and only effective for some patients, and the product is a live bacterial preparation, the number of viable bacteria gradually decreases during storage, and the effect becomes more and more effective. Difference

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  • Kefir whey and tea polyphenol composition as well as preparation method and application thereof
  • Kefir whey and tea polyphenol composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preparation of kefir whey

[0025] The whole milk powder was prepared with sterile water with a concentration of 7% reduced milk, sterilized in a water bath at 80°C for 20 minutes, and after natural cooling, under aseptic operation, the kefir grains were prepared according to the concentration of 5% by mass. % added to the sterilized reduced milk solution, and fermented at constant temperature at 20, 25, 30, and 37°C for 7, 20, 30, and 45 days under sealed conditions. Taking streptomycin sulfate as the control, when the titer of the fermentation supernatant exceeded 1700 IU Fermentation was terminated at the time of / ml, the fermentation broth was centrifuged at 5000rpm for 10min, and then the supernatant was passed through a 0.22 μm filter to obtain the initial whey extract. The kefir whey extract was obtained by evaporating and concentrating under reduced pressure at 80° C., after 50%, 60%, 70% and 80% of the water was evaporated, which was used in the foll...

Embodiment 2

[0026] Example 2: Preparation of kefir whey and tea polyphenol composition

[0027] Take the 30 ℃ of constant temperature fermentation in Example 1 for 45 days, centrifugal filtration to obtain the initial whey extract, use the rotary evaporator to evaporate and concentrate under reduced pressure under the condition of 60 ℃, and after 50% of the water is evaporated, the whey initial extract is obtained. Phil whey working solution, add tea polyphenols into kefir whey working solution, stir and mix evenly, prepare a solution with a mass percentage concentration of 0.05%-0.5% of tea polyphenols, and distribute the obtained solution into glass bottles , and sterilized in a water bath at 80° C. for 15 minutes to obtain a kefir whey and tea polyphenol composition.

Embodiment 3

[0028] Example 3: Take the kefir whey and tea polyphenol composition solution with a tea polyphenol mass percentage concentration of 0.1% in Example 2, and the effect of the composition on the intestinal flora and inflammatory response of colitis mice.

[0029] Preparation of experimental animals:

[0030] Selected 72 SPF grade C57BL / 6 male mice (4-5 weeks old), all purchased from the Guangdong Provincial Medical Animal Experiment Center, the license number is SCXK (Guangdong) 2018-0002, the initial weight is about 20g, in the SPF grade The experimental animals were housed in a room with a day and night cycle of 12 hours each to maintain a constant temperature and humidity in the environment. Standard rat chow and water were used for animal feeding.

[0031] Establishment of a mouse colitis model:

[0032] 72 SPF C57BL / 6 mice were adaptively fed for 1 week. Randomly divided into 6 groups (n=12 /

[0033] Group). The blank control group (Con) mice were fed with normal chow a...

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Abstract

The invention belongs to the technical field of biotechnology, and discloses a kefir whey and tea polyphenol composition as well as a preparation method and application thereof. The method comprises the following operation steps: preparing a reduced milk solution from whole milk powder by using sterile water, sterilizing in a water bath, naturally cooling, adding kefir grains into the sterilized reduced milk under sterile operation, fermenting for 7-45 days at the constant temperature of 15-30 DEG C under a sealed condition, centrifuging, and filtering to obtain a kefir whey primary extracting solution; the preparation method comprises the following steps: preparing a Kefir whey working solution, carrying out reduced pressure evaporation and concentration to obtain a Kefir whey working solution, adding tea polyphenol into the Kefir whey working solution to prepare a solution with the mass percentage concentration of tea polyphenol being 0.05-0.5%, carrying out split charging, and carrying out water bath sterilization to obtain the Kefir whey and tea polyphenol composition. The composition has a very good effect of treating the inflammatory bowel disease, and the kefir whey and the tea polyphenol are derived from edible raw materials, so that the product is edible, high in safety, simple in production process and relatively low in cost.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and particularly relates to a kefir whey and tea polyphenol composition and a preparation method and application thereof. Background technique [0002] Inflammatory bowel disease (IBD) is a complex gastrointestinal disease, mainly including ulcerative colitis (UC) and Crohn's disease (CD). Recurrent and pathogenic inflammation is the main feature. Epidemiological studies have shown that the incidence and prevalence of IBD are accelerating globally. The clinical manifestations of UC are persistent and repeated episodes of diarrhea, the most common symptom being mucus, pus and blood in the stool accompanied by abdominal pain. The clinical manifestations of CD are diverse. The most common symptoms are diarrhea, abdominal pain, and sometimes blood in the stool, accompanied by weight loss, fever, loss of appetite, fatigue, anemia, etc., and adolescent patients will have delayed growth and dev...

Claims

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Application Information

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IPC IPC(8): A61K35/20A61K31/353A61K36/82A61P1/00A61P29/00
CPCA61K35/20A61K31/353A61K36/82A61P1/00A61P29/00A61K2300/00
Inventor 方祥方子莹熊嘉麒廖振林王洁钟青萍
Owner SOUTH CHINA AGRI UNIV
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