Magnetic bead method nucleic acid extraction reagent formula capable of effectively improving virus nucleic acid recovery efficiency

Abstract

The invention discloses a magnetic bead method nucleic acid extraction reagent formula capable of effectively improving virus nucleic acid recovery efficiency. Comprising urea, thiourea, thiothreitol, ethylenediamine tetraacetic acid, cholamidopropyl, tromethamine, ethyl phenyl polyethylene glycol, polyethylene glycol octyl phenyl ether, phenylmethylsulfonyl fluoride, sodium chloride, sodium fluoride, guanidine hydrochloride, guanidine isothiocyanate, protease K, sodium deoxycholate, distilled water, a protease inhibitor, a phosphatase inhibitor and sodium orthovanadate. The preparation method mainly comprises the following steps: adding guanidine hydrochloride into urea to achieve a solubilizing effect on amino acid, adding protease K to cut carboxyl-terminal peptide bonds of aliphatic amino acid and aromatic amino acid, adding a protease inhibitor to effectively inhibit various asparaginic acid proteases, and adding a phosphatase inhibitor to inhibit various asparaginic acid proteases. Alkaline phosphatase and tyrosine phosphatase can be inhibited, the recovery efficiency of viral nucleic acid can be effectively improved, the working efficiency is improved, and the method is convenient and practical.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a formula of a magnetic bead nucleic acid extraction reagent that can effectively improve the recovery efficiency of viral nucleic acid. Background technique [0002] Magnetic bead nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, mainly reflected in the ability to realize automation and large-scale operations, which meet the requirements of high-throughput biological operations, enabling rapid and timely response to outbreaks of infectious diseases. Magnetic bead nucleic acid extraction is a new nucleic acid extraction technology based on nano-biological magnetic beads. Nucleic acid molecules can specifically recognize and combine with the silanol on the surface of the magnetic beads, and aggregate or disperse under the action of an external magnetic field. Get rid of manual operations such as centrifugation and supernat...

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Problems solved by technology

[0002] Magnetic bead nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, mainly reflected in the ability to realize automation and large-scale operations, which meet the requirements of high-throughput biological operations, enabling rapid and timely response to outbreaks of infectious diseases. Magnetic bead nucleic acid extraction is a new nucleic acid extraction technology based on nano-biological magnetic beads. Nucleic acid molecules can specifically recognize and combine with the silanol on the surface of the magnetic beads, and aggregate or disperse under the action of an external magnetic field. Get rid of manual operations such as centrifugation and supernatant extraction in the traditional nucleic acid extraction process, so as to realize the automatic extraction of nucleic acids, which are widely used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, molecular biology research, etc. In many fields, magnetic bead nucleic acid extraction generally includes four main steps of lysis, binding, washing, and elution, and each step can be realized by a variety of different methods alone or in combination. In order to improve the efficiency of viral nucleic acid recovery, the lysis link Nucleic acid extraction reagents are the most important thing to determine the quality of nucleic acid extraction, and the extraction effect of nucleic acid extraction reagents in the prior art is relatively general, which reduces the efficiency of virus nucleic acid recovery, increases the working hours of nucleic acid extraction, and reduces work efficiency. Therefore, it is necessary to Use the magnetic bead method nucleic acid extraction reagent formula that can effectively improve the efficiency of virus nucleic acid recovery to meet the needs of use

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