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Tetragenococcus halophilus and application of tetragenococcus halophilus in production of anti-cancer exopolysaccharides

A technology of halophilic tetradococcus and exopolysaccharide, which is applied in the field of new halophilic tetradococcus and its application in the production of anti-cancer exopolysaccharide, which can solve the problems of body damage and side effects

Active Publication Date: 2022-03-18
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current clinical surgical treatment is mainly suitable for early cancer patients, and chemotherapy and radiotherapy are often used for advanced cancer patients. However, most of the current clinical chemotherapy drugs have great side effects of body damage, so there is an urgent need for a more advanced cancer treatment. effective method

Method used

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  • Tetragenococcus halophilus and application of tetragenococcus halophilus in production of anti-cancer exopolysaccharides
  • Tetragenococcus halophilus and application of tetragenococcus halophilus in production of anti-cancer exopolysaccharides
  • Tetragenococcus halophilus and application of tetragenococcus halophilus in production of anti-cancer exopolysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The isolation and screening of embodiment 1 bacterial strain

[0064] 1. Screening samples

[0065] The experimental samples were 192 naturally fermented soybean paste samples collected from 12 different regions in Liaoning Province (Jinzhou, Shenyang, Zhuanghe, Benxi, Dalian, Gongzhuling, Dandong, Huludao, Tieling, Anshan, Liaozhong and Liaoyang).

[0066] 2. Preliminary screening of tetradococci

[0067] Take 1g of soybean paste sample, add 9mL of 0.9% sterile normal saline, mix thoroughly and soak for 15min; then take 1mL of sample solution and inoculate it into the enrichment culture set, and after static culture at 30°C for 1-2d, use the plate coating method Cultured on solid separation medium for 5-6d.

[0068] Pick a single colony that is small, has obvious calcium-dissolving circle, is milky white and opaque, and has a shape similar to that of a lactic acid bacteria colony, and performs Gram staining. The results of microscopic examination showed that there w...

Embodiment 2

[0079] Identification of embodiment 2 LZ4 bacterial strain

[0080] 1. Identification of colony morphology

[0081] The colony of the LZ4 strain is small, milky white, opaque, smooth and glossy, sticky, with neat edges; Gram staining is positive, the cells are spherical, and generally exist in quadruple or in pairs. Colony morphology and cell morphology figure 1 and figure 2 shown.

[0082] 2. Physiological and biochemical characteristics

[0083] Physiological and biochemical characteristics of the LZ4 strain were tested and identified: sugar fermentation test; catalase test; gelatin liquefaction test; ammonia production test; glucose production acid and gas test; hydrogen sulfide test;

[0084] 3. Molecular biological identification

[0085] (1) Bacterial DNA extraction:

[0086] Pipette 100 μL of the bacterial suspension of the LZ4 strain into the sterilized MRS liquid medium, and culture it in a shaking incubator at 37°C for 24 hours. The genome of the strain was e...

Embodiment 3

[0100] Example 3 Analysis of Fermentation Conditions of Tetradendococcus halophilus SNTH-8

[0101] 1. Effect of carbon source and its concentration on polysaccharide synthesis and strain growth

[0102] On the basis of sugar producing medium, 2% (w / v) glucose, sucrose, mannose, fucose, galactose, arabinose, rhamnose and xylose were respectively used as carbon sources to determine the For polysaccharide production and strain biomass, the optimal carbon source was determined according to the production and growth of exopolysaccharide of the strain, and its optimal concentration was investigated.

[0103] From Figure 5 It can be seen that the halophilic Tetradendococcus SNTH-8 cannot ferment rhamnose and xylose to produce sugar, and the growth of the strain is not good. Although glucose can promote the growth of the strain to a certain extent, the production of exopolysaccharide is less than that of sucrose. Therefore, sucrose is the best carbon source among the tested carbo...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a novel tetragenococcus halophilus and an application of the novel tetragenococcus halophilus in production of anti-cancer extracellular polysaccharide. The tetracoccus halophilus is separated and screened from natural fermented soybean paste in northeast China, the preservation number is CGMCC No.23137, and the tetracoccus halophilus can produce exopolysaccharides at high yield and has the advantages of being short in sugar production period, safe and free of toxic and side effects. The exopolysaccharide produced by the strain can significantly inhibit human hepatoma carcinoma cell proliferation and induce cell apoptosis, and is expected to be developed into a new anti-cancer natural drug.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening and application, in particular to a novel strain of halophilic tetradococcus and its application in the production of anti-cancer extracellular polysaccharides Background technique [0002] In China, cancer is still the number one cause of death, and the morbidity and mortality are still rising every year. According to the 2021 China Cancer Statistics released by the National Cancer Center, it is estimated that there will be 10,000 new cancer cases and deaths in China in 2021. Liver cancer As the fourth cause of cancer-related death after lung cancer, colon cancer and gastric cancer, it is still a common tumor with high morbidity and mortality in my country. The current clinical surgical treatment is mainly suitable for early cancer patients, and chemotherapy and radiotherapy are often used for advanced cancer patients. However, most of the current clinical chemotherapy d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04A61K31/715A61P35/00A23K10/18A23L29/00A23L29/30A23L33/125A23L33/135C12R1/01
CPCC12P19/04A23L29/065A23L33/135A23L33/125A23L29/30A23K10/18A61K31/715A61P35/00A23V2002/00A23V2250/51A23V2200/308
Inventor 乌日娜武俊瑞徐菁雯魏丽丽杨雪萌
Owner SHENYANG AGRI UNIV
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