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Amino magnetic nanoparticle coating method and application thereof

A magnetic nanometer and magnetic particle technology, applied in chemical instruments and methods, other chemical processes, alkali metal oxides/hydroxides, etc., can solve the problems of not meeting the requirements of potency and low potency

Pending Publication Date: 2022-03-11
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional polyacrylic acid magnetic nanoparticles are coated with low potency, which does not meet the potency requirements in the field of in vitro diagnostics

Method used

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  • Amino magnetic nanoparticle coating method and application thereof
  • Amino magnetic nanoparticle coating method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Coated magnetic particle HE 4 (human epididymis protein 4) process:

[0025] Take Merck amino magnetic nanoparticles (100mg / ml, 40μl), wash 5 times (0.1MPBS 400μl×5 times) to remove the supernatant, add 50μl (1mg / ml, dissolved in MES buffer) polyacrylic acid solution and 50μl (20mg / ml, the EDC solution of MES (that is, 2-(N-morpholine) ethanesulfonic acid) buffer solution), the rapid mixer shakes evenly, and places on the shaker for 2h;

[0026] Use a magnet to remove the supernatant, add coating solution 85μl MES buffer and 15ulHE 4 Coat live materials, vibrate evenly with a fast mixer and place on an oscillator to vibrate for 2 hours;

[0027] Use HE 4 The sealing solution was washed 5 times (400 μl × 5 times), and the sealing solution was fixed to 3 mL; the sealing solution included 1000 mL of 0.1M phosphate buffer (pH=7.4) with 10 g of BSA and 1 mL of Tween 20 (surfactant ) and 1 mL of sodium azide (preservative).

Embodiment 2

[0028] Embodiment 2: the process of coating magnetic particle CA199:

[0029] Take Merck amino magnetic nanoparticles (100mg / ml, 40μl), wash 5 times (0.1M PBS 400μl×5 times) to remove the supernatant, add 50ul (1mg / ml, dissolved in PBS) polyacrylic acid solution and 50μl (10mg / ml ml, dissolved in 0.1M PBS) of EDC solution, oscillate evenly with a rapid mixer, and then place it on the shaker for 2h;

[0030] Remove the supernatant after magnetic suction with a magnet, add coating solution 85 μl 0.1M PBS buffer and 15 μl CA199 (carbohydrate antigen 199) to coat the living material, shake it evenly with a fast mixer, and place it on the shaker for 2 hours;

[0031] Use CA199 sealing solution to wash 5 times (400μl×5 times), and seal to 3mL; the sealing solution includes 1000mL of 0.1M phosphate buffer (pH=7.4) with 10gBSA and 1mL of Tween 20 (surfactant agent) and 1 mL of sodium azide (preservative).

[0032] The present invention adopts Antu Biological A2000Plus detection inst...

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Abstract

The invention provides an amino magnetic nanoparticle coating method and application thereof, and the coating method comprises the following steps: cleaning amino magnetic nanoparticles, removing supernate, adding a polyacrylic acid solution and an EDC solution, and oscillating to obtain a solution containing polyacrylic acid modified Merck amino magnetic nanoparticles; carrying out magnetic attraction on the solution containing the polyacrylic acid modified Merck amino magnetic particles, adding a coating solution and a coating active material, shaking again to carry out a coupling reaction, carrying out magnetic attraction again, removing supernatant by adopting a sealing and protecting solution, and storing by using the sealing and protecting solution, so as to obtain the coated amino magnetic nanoparticles. According to the invention, polyacrylic acid is adopted to modify the magnetic nanoparticles of which the surfaces are modified into amino groups, so that agglutination in the coating process and agglutination of a magnetic suction carrier can be improved, and meanwhile, the stability after coating can also be improved; the quality of the in-vitro diagnostic kit can be improved, and the use experience of customers is improved.

Description

technical field [0001] The invention belongs to the technical field of functional polymer modification, and in particular relates to a coating method and application of amino magnetic nano particles. Background technique [0002] Magnetic nanoparticles refer to colloidal composite materials that can be uniformly dispersed in a certain base fluid. Because of its superparamagnetic properties, high specific surface area, and functional groups that can be modified. Because of its unique characteristics, it has a wide range of applications in the field of biomedicine, such as magnetic targeting drugs, cell or DNA separation and immunodiagnosis, magnetic resonance imaging, etc. Among them, in terms of immunodiagnosis, magnetic nanoparticles are an indispensable part of in vitro diagnostic reagents as living material carriers. The functional groups on the surface of common magnetic nanoparticles mainly include carboxyl, amino, hydroxyl and tosyl groups, among which the surface mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30
CPCB01J20/28009B01J20/28011B01J20/265
Inventor 李思雨王慧敏余春霞李冰陈永奇杨阳王玉堂李彬
Owner AUTOBIO DIAGNOSTICS CO LTD
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