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Fingerprint spectrum construction method and application of Chinese herbal compound containing cinnamon

A technology of traditional Chinese medicine and compound prescription, which is applied in the field of fingerprint construction of traditional Chinese medicine compound to achieve the effect of ensuring the stability of chemical composition and safety of use, good separation and good repeatability

Pending Publication Date: 2022-03-08
HUNAN YINENG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no reports in the literature on the comprehensive analysis and fingerprinting of the chemical components of the above-mentioned compound.

Method used

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  • Fingerprint spectrum construction method and application of Chinese herbal compound containing cinnamon
  • Fingerprint spectrum construction method and application of Chinese herbal compound containing cinnamon
  • Fingerprint spectrum construction method and application of Chinese herbal compound containing cinnamon

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0083] The preparation of need testing solution: get and comprise the traditional Chinese medicine compound powder 0.5g of cinnamon, accurately weigh, place in conical flask, precision add volume percent concentration and be 50% methanol aqueous solution is 50ml, weigh, ultrasonic extraction 30 minutes, Cool to room temperature, make up the lost weight with 50% methanol aqueous solution with a concentration of 50% by volume, shake well, filter, and take the subsequent filtrate as the test solution.

[0084] Alternatively, take 0.5g of Chinese medicine compound powder containing cinnamon, accurately weigh it, place it in a 50mL volumetric flask, accurately add an appropriate amount of methanol aqueous solution with a volume percentage concentration of 50%, ultrasonically extract for 30 minutes, cool to room temperature, and use a volume percentage concentration of 50%. % methanol aqueous solution to the mark, shake well, filter, and take the subsequent filtrate as the test solut...

Embodiment 1

[0091] Example 1: Detection wavelength selection Gallic acid, paeoniflorin, β-ecdysterone, ferulic acid, liquiritin, cinnamon aldehyde, paeonol, ammonium glycyrrhizinate, ginsenoside Rg1 and ginsenoside Re are traditional Chinese medicine compound containing cinnamon The 10 active ingredients in the test have obvious differences in molecular structure, polarity and spectral characteristics, and they are representative as index control ingredients. According to the characteristic absorption of the ultraviolet light spectrum of these 10 kinds of components, from the three-dimensional atlas of DAD (such as figure 1 Shown) to extract the chromatograms under different wavelengths for comparison, the results show that the chromatogram baseline obtained under 252nm is stable, and can take into account the detection sensitivity of each component, so it is selected as the detection wavelength of fingerprint and content determination; the obtained under 203nm The baseline of the chromat...

Embodiment 2

[0092] Example 2: Under the same other conditions, compare Agilent 5TC-C18 (4.6mm×250mm, 5μm), Supersil ODS2 (4.6mm×250mm, 5μm), Agilent ZORBAXSB-Phenyl (250×4.6mm, 5μm), etc. The separation effect of the three chromatographic columns shows that there are significant differences in the number of peaks and peak resolution of each chromatographic column, among which Agilent ZORBAXSB-Phenyl (250×4.6mm, 5μm) has the best performance indicators, so Agilent ZORBAXSB- Phenyl (250×4.6mm, 5μm) was used as a determination column for fingerprint spectrum and content determination.

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Abstract

The invention discloses a detection method of a Chinese herbal compound containing cinnamon, and the detection method comprises the following steps: a Chinese herbal compound test solution and a reference solution are taken for detection, the Chinese herbal compound is composed of angelica sinensis, ligusticum wallichii, radix paeoniae alba, cinnamon, moutan bark, curcuma zedoary, ginseng, liquorice and radix achyranthis bidentatae; the reference substances are gallic acid, paeoniflorin, beta-ecdysterone, ferulic acid, liquiritin, cinnamaldehyde, paeonol, ammonium glycyrrhizinate, ginsenoside Rg1 and ginsenoside Re, and the chromatographic conditions of the detection are as follows: a chromatographic column with phenyl bonded silica gel as a filler is adopted, a mobile phase A is selected from one or more of acetonitrile, methanol and tetrahydrofuran, a mobile phase B is selected from one or more of acetonitrile, methanol and tetrahydrofuran, and a mobile phase C is selected from one or more of acetonitrile, methanol and tetrahydrofuran; the mobile phase B is an acid aqueous solution or a buffer salt aqueous solution, and obtaining component information or component and content information of the traditional Chinese medicine compound according to a detection result. The chemical components of the traditional Chinese medicine compound containing cinnamon are comprehensively and systematically analyzed, and a theoretical basis is provided for deep research on quality control and pharmacodynamic material basis.

Description

technical field [0001] The present application relates to the technical field of quality identification of traditional Chinese medicines, in particular to a method for constructing fingerprints of Chinese medicine compound containing cinnamon and its application. Background technique [0002] With the rapid development of traditional Chinese medicine preparations in my country, the quality management of traditional Chinese medicine preparations has also received widespread attention, and its quality will directly affect the quality of medical care and the safety of patients. The fingerprint of traditional Chinese medicine (including single traditional Chinese medicine and traditional Chinese medicine compound) refers to the chromatogram or spectrum that can mark the chemical characteristics of some Chinese medicinal materials or traditional Chinese medicine preparations after appropriate processing and certain analysis methods. Traditional Chinese medicine fingerprint is a c...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8686G01N2030/047Y02A50/30
Inventor 易跃能聂作兵杨帆朱亚文魏丹丹陈锋
Owner HUNAN YINENG BIOLOGICAL PHARMA
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