Method for extracting ganoderma lucidum sporocarp polysaccharide through fermentation pretreatment

Abstract

The invention discloses a method for extracting ganoderma lucidum sporocarp polysaccharide through fermentation pretreatment. The method comprises the following steps of: S1, fermentation strain preparation; S2, ganoderma lucidum sporocarp pretreatment; s3, ganoderma lucidum powder pretreatment; S4, liquid state fermentation or solid state fermentation; and S5, fermentation product extraction. With the method adopted, the polysaccharide content and the polysaccharide extraction rate of a ganoderma lucidum extract can be improved, the ganoderma lucidum extraction is facilitated after the fermentation pretreatment, the subsequent extraction times can be reduced, and the extraction utilization rate of the ganoderma lucidum is improved.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of natural products, in particular to a method for extracting ganoderma fruiting body polysaccharides through fermentation pretreatment. Background technique [0002] Ganoderma lucidum generally refers to the fruiting body of Ganoderma lucidum (Leyss. ex. Fr) Karst, which belongs to the polyporaceae fungus. It is a traditional rare medicinal material in my country. It can be used clinically as adjuvant chemotherapy and / or radiotherapy to treat tumors, chronic bronchitis and asthma. Neurasthenia, insomnia, hyperlipidemia, hypertension, diabetes, hepatitis, leukopenia, poisonous mushroom poisoning and other aspects of treatment or adjuvant treatment. Ganoderma lucidum contains a variety of functional active ingredients. Among them, Ganoderma lucidum polysaccharide has many physiological activities such as immune regulation, anti-tumor, anti-oxidation, lowering blood sugar, lowering ...

AI Technical Summary

Problems solved by technology

[0005] 1. Trichoderma viride is not a strain that can be directly used in food or health food. Its polysaccharide preparation method is to add methanol to its Ganoderma lucidum fermentation liquid, and take its precipitated part. The mold body is a macromolecule, and it is definitely not an alcohol-soluble substance. A considerable part of the bacteria is also transferred to its alcohol precipitation site, so the safety of the obtained polysaccharide mixed with Trichoderma viride bacteria in oral products such as medicines, health care products, and food is doubtful, and this method is used in the purification process of polysaccharides. Methanol, as we all know, methanol is a highly toxic substance. A low dose can cause permanent damage to the visual system, and a large dose can even cause poisoning death. Therefore, there is a great potential safety hazard in the preparation of polysaccharides by this method, and the product is not suitable for The large-scale commercial production of daily oral or daily chemical products can only be applied in the field of scientific research

[0006] 2. The fermentation liquid of this method is directly purified by methanol to prepare polysaccharides without extraction. After fermentation, the material cannot fully release its polysaccharides without extraction, so it will cause waste of polysaccharides and even Ganoderma lucidum raw materials. In addition, this method adds 1-7V% of Trichoderma viride fermented liquid, the ratio of ganoderma lucidum fruiting body powder to distilled water is 1:20 (w / v), the amount of fermented liquid added is very large, and the average value of 1-7V% is added at 3.5V%, then Trichoderma viride The amount of fermentation liquid added is 70% (v / w) of Ganoderma lucidum, and the maximum value is 7V%. Even if the amount of auxiliary fermentation liquid exceeds the amount of raw materials, the minimum amount of 1v% is added to account for 20% (v / w) , the amount of fermentation aids used in this method is too large, and the rationality of choosing Trichoderma viride as the fermentation strain of Ganoderma lucidum is low, and the strain does not have a high efficiency improvement for the fermentation of Ganoderma lucidum, and the industrialized large-scale production is enlarged, which increases production costs

[0007] 3. This method directly adds toxic organic chemical solvents to the fermentation broth with 20 times the amount of raw materials for refining and purification. Assuming that the alcohol concentration of the solution is only adjusted to 50%, in general research, application, and detection methods, alcohol-precipitated polysaccharides need to reduce the concentration of alcohol-precipitated liquid To adjust the alcohol concentration to ≥ 75%, it is also necessary to add methanol 10 times the amount of raw materials, so the amount of fermentation broth and toxic solvents used in this method are large, and there is no sterilization treatment before the fermentation of Ganoderma lucidum. Shaking bed culture for 36 hours, during which it is impossible to inhibit the growth of the miscellaneous bacteria contained in Ganoderma lucidum itself, on the one hand, it will interfere with the investigation of the polysaccharide extraction effect, that is, the influence of the fermentation of the miscellaneous bacteria brought by Ganoderma lucidum itself on the preparation of polysaccharides cannot be ruled out. On the other hand, the hygienic safety of the product is greatly reduced, which limits the commercial application of its craft products

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