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Derris centralis extract bactericide as well as preparation method and application thereof

A technology of extracts and fungicides, which is applied in the field of degeria genus extracts and fungicides and its preparation, can solve problems such as the lack of systematic research on the prevention and treatment of plant pathogenic fungi, and achieve broad-spectrum antifungal activity, low residue, and low cost. Effect

Active Publication Date: 2021-12-17
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies on Deerhinensis are on the insecticidal activity, and there is no systematic research on the control of plant pathogenic fungi.

Method used

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  • Derris centralis extract bactericide as well as preparation method and application thereof
  • Derris centralis extract bactericide as well as preparation method and application thereof
  • Derris centralis extract bactericide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1-Preparation of Deerhide vine extract fungicide

[0020] Take fresh Deutschia zhongnan branches and leaves, cut them into 9cm branch and leaf sections, dry them in an oven at 45°C, and then pulverize them with a pulverizer to obtain the branches and leaves of Zhongnan vines; according to the dosage ratio of 1.5g:30mL, take Add 1.5kg of deer vine branches and leaves powder, add 30L of methanol solution with a mass concentration of 80%, stir, soak for 48 hours, filter, add again a methanol solution with a mass concentration of 80%, repeat soaking twice, filter, and combine the filtrates to obtain a soaking solution. Using a rotary evaporator to concentrate under vacuum at 45°C, the methanol extract 1 of the branches and leaves of Deerhinensis was obtained;

[0021] Take fresh Deutschia zhongnan branches and leaves, cut them into 10cm branch and leaf sections, dry them in an oven at 50°C, and then pulverize them with a pulverizer to obtain the branches and leav...

Embodiment 2

[0024] Example 2-in vitro antifungal activity test

[0025] The bactericidal activity of the fungicides extracted from Deerhide vines was detected by the method of mycelial growth rate. Take by weighing quantitative Deerhinen chinensis extract bactericide, after fully dissolving with DMF, be mixed with the test drug solution, the test drug solution and the potato dextrose agar medium (PDA medium) are mixed, and the concentration of the extract is made into 2.00mg·mL -1 The drug-carrying medium was used as a control with the PDA medium with the same content of DMF. The pathogenic fungi were made into fungus cakes with a puncher with a diameter of 0.5 cm, inserted into the middle of the medium with the medicine, and three replicates were set. The transferred pathogenic bacteria were cultured in a constant temperature incubator at 28°C. When the diameter of the colony in the control group reached 5.0 cm or more, the diameter of the colony was measured by the cross method. The ...

Embodiment 3

[0033] Example 3-Determination of inhibitory activity of conidia germination of banana anthracnose bacteria

[0034] Weigh the quantitative methanol extract 2, petroleum ether phase extract, ethyl acetate phase extract, n-butanol phase extract and aqueous phase extract of the branches and leaves of Deereus chinensis, dissolve them in DMF, and configure the test solution. Add to PDA medium and mix well to make 1.0mg·mL -1 drug-carrying medium. Draw 1.0 mL of the drug-containing medium and place it on a glass slide. After solidification, smear the banana anthracnose spore suspension (1×10 7 cfu mL -1 ), with sterile water containing the same proportion of DMF as a control, each treatment set 3 repetitions, and the experiment was repeated 3 times. The treated glass slides were cultured in a constant temperature incubator at 28°C. The spore germination rate of the control group exceeded 95.00%. After that, the spore germination of each treatment component was observed under a ...

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Abstract

The invention provides an application of a derris centralis extract bactericide to plant pathogenic fungi. The derris centralis extract bactericide is a methanol extract of branches and leaves of derris centralis or an organic solvent extract of the methanol extract of the branches and leaves of derris centralis. The derris centralis extract bactericide disclosed by the invention not only has broad-spectrum antifungal activity, but also has a good control effect on banana anthracnose and cowpea powdery mildew, has the advantages of low toxicity, low residue, environment friendliness and low cost, is simple in preparation process and easy to control in operation method, can be used as a bactericide for plant fungal diseases, and can reduce the use of chemical bactericides. Therefore, the derris centralis has potential application value in the aspect of preventing and treating fungal diseases of plants.

Description

technical field [0001] The invention relates to the field of pesticide fungicides, in particular to a fungicide extracted from Deerhide chinensis and its preparation method and application. Background technique [0002] When our country is one of the big agricultural countries in the world, crops have been threatened by diseases, insect pests and weeds since ancient times, which often have a serious impact on agricultural production. At present, chemical control of plant diseases, insect pests and weeds is the main method. The use of chemical pesticides has reduced the threat of agricultural diseases and insect pests to a certain extent. Negative impacts such as problems are increasing day by day. With the improvement of people's living standards and environmental protection awareness, safe, green and efficient bio-pesticides and bio-control technologies and concepts are more and more respected by people. Botanical pesticides have the characteristics of low toxicity, high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/20A01P3/00
CPCA01N65/00A01N65/20
Inventor 孙然锋刘启凤胡展谢佳
Owner HAINAN UNIVERSITY
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