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Stable free fatty acid determination kit

A technology of free fatty acids and kits, applied in the determination/inspection of microorganisms, measurement of color/spectral characteristics, biochemical equipment and methods, etc.

Pending Publication Date: 2021-12-10
上海信利健康管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a stable free fatty acid assay kit to solve the problem of how to solve the problem based on the disclosure of the Chinese patent document "a free fatty acid assay kit and its preparation method (patent application number is CN201610831420.3)" Optimize the components, dosage, process, etc., so as to improve the stability of the stable free fatty acid assay kit

Method used

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Effect test

Embodiment 1

[0036] A stable free fatty acid assay kit, comprising reagent R1 and reagent R2,

[0037] Reagent R1 contains the following ingredients: sodium dihydrogen phosphate 50mmol / L, coenzyme A 6mmol / l, ATP3mmol / L, acyl-CoA synthetase 0.8KU / L, bovine whey albumin 15g / L, casein sodium salt 10g / L, Sodium octanoate 10g / L, Trinder substrate 1.2g / L, Tween 20 45ml / L, enzyme protection agent 6ml / L; enzyme protection agent is dipotassium edetate and magnesium sulfate with a concentration ratio of 1:1 mixture;

[0038] Reagent R2 contains the following ingredients: sodium dihydrogen phosphate 60mmol / L, flavin adenine dinucleotide 2mmol / L, 4-aminoantipyrine 6mmol / L, peroxidase 28KU / L, acyl-CoA oxidation Enzyme 7KU / L, Tween 20 35ml / L sodium azide 2ml / L, complex thiol reagent 150ml / L, chromogen 0.5mmol / L, dodecyl polyethylene glycol ether 1.3g / L, concentrated hydrochloric acid 3ml / L L. The chromogen is a mixture of TOPS and DAOS at a ratio of 1:1.

[0039] The preparation method of the reagen...

Embodiment 2

[0042] A stable free fatty acid assay kit, comprising reagent R1 and reagent R2,

[0043] Reagent R1 contains the following ingredients: glycine 40mmol / L, coenzyme A 1mmol / l, ATP 1mmol / L, acyl-CoA synthetase 1KU / L, bovine whey albumin 15g / L, casein sodium salt 15g / L, sodium octanoate 15g / L, Trinder substrate 1.1g / L, EMULGEN A-60 55ml / L, enzyme protection agent 10ml / L; The enzyme protection agent is that the concentration ratio of dipotassium edetate and magnesium sulfate is 1:1 mixture;

[0044] Reagent R2 contains the following ingredients: glycine 40mmol / L, flavin adenine dinucleotide 1mmol / L, 4-aminoantipyrine 6mmol / L, peroxidase 35KU / L, acyl-CoA oxidase 9KU / L L. EMULGEN A-60 5ml / L, Potassium Sorbate 1ml / L, Compound Sulfhydryl Reagent 145ml / L, Chromogen 0.1mmol / L, Dodecyl Polyethylene Glycol Ether 1.5g / L, Concentrated Hydrochloric Acid 3ml / L . The chromogen is a mixture of TOPS and DAOS at a ratio of 1:1.

[0045] The preparation method of the reagent R1 is as follows: ...

Embodiment 3

[0048] A stable free fatty acid assay kit, comprising reagent R1 and reagent R2,

[0049] Reagent R1 contains the following ingredients: sodium dihydrogen phosphate 80mmol / L, coenzyme A 7mmol / l, ATP 3mmol / L, acyl-CoA synthetase 0.4KU / L, bovine whey albumin 20g / L, casein sodium salt 5g / L , sodium octanoate 5g / L, Trinder substrate 1.5g / L, EMULGEN A-90 80ml / L, enzyme protection agent 2ml / L; The enzyme protection agent is the concentration ratio of dipotassium edetate and magnesium sulfate 1:1 mixture;

[0050] Reagent R2 contains the following ingredients: sodium dihydrogen phosphate 80mmol / L, flavin adenine dinucleotide 2mmol / L, 4-aminoantipyrine 8mmol / L, peroxidase 20KU / L, acyl-CoA oxidation Enzyme 10KU / L, EMULGEN A-90 60ml / L, sodium benzoate 2ml / L, compound thiol reagent 200ml / L, chromogen 0.7mmol / L, dodecyl polyethylene glycol ether 1g / L, concentrated hydrochloric acid 5ml / L L. The chromogen is a mixture of TOPS and DAOS at a ratio of 1:1.

[0051] The preparation method ...

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Abstract

The invention discloses a stable free fatty acid determination kit, and belongs to the field of medical equipment. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components: a buffer solution, coenzyme A, ATP, acyl coenzyme A synthetase, bovine whey albumin, casein sodium salt, sodium n-octoate, a Trinder substrate, a surfactant and an enzyme protective agent; the reagent R2 is prepared from the following components: a buffer solution, flavin adenine dinucleotide, 4-aminoantipyrine, peroxidase, acyl coenzyme A oxidase, a surfactant, a preservative, a composite sulfydryl reagent, chromogen, dodecyl polyglycol ether and concentrated salt. The composite sulfydryl reagent, the bovine whey albumin and the casein sodium salt are added into the kit, the composite sulfydryl reagent can react with sulfydryl of coenzyme A to remove interference of the coenzyme A on chromogen, the casein sodium salt can be combined with the bovine whey albumin, and the stability of the kit is improved.

Description

technical field [0001] The invention belongs to the field of medical equipment, in particular to a stable free fatty acid assay kit. Background technique [0002] Free fatty acid (free fatty acid) FFA, also known as non-esterified fatty acid, generally refers to fatty acids above C10 in clinical practice. One of the important energy substances. Lipid substances are the main components of cell membranes and have a variety of important biological functions. Lipids present in the human body can be roughly divided into four types: cholesterol, neutral fat (triglycerides), phospholipids, and free fatty acids. Free fatty acids are substances that neutral fats are broken down into. When the energy required for muscle activity - liver cheese is exhausted, adipose tissue will decompose neutral fat into free fatty acids to be used as energy. Therefore, free fatty acids can be said to be substances required for sustained activities. Free fatty acids in normal serum mainly include ...

Claims

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Application Information

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IPC IPC(8): C12Q1/00C12Q1/30
CPCC12Q1/48C12Q1/28C12Q1/26G01N21/31C12Q2326/96
Inventor 黄孟
Owner 上海信利健康管理有限公司
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