New auricularia cornea strain and application thereof

A strain and fungus technology, applied in the field of microorganisms, to achieve the effect of high total amino acid content and strong antibacterial ability

Active Publication Date: 2021-11-19
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Guangxi is located in a subtropical monsoon climate, with many mountains and lush forests. The summer is cooler and the climate is warm and humid, which is very suitable for the growth of edible fungi. Therefore, there are abundant fungus resources in Guangxi. In recent years, more and more fungus strains with excellent quality have been produced. It has been collected and found, but there are still many excellent varieties of wild resources to be developed and applied. Therefore, in order to further enrich the varieties of fungus, enrich the germplasm resources of fungus, and separate and domesticate more and better varieties from the wild, it is necessary to devote ourselves to Collection, separation and purification, domestication and research and development of fungus strains

Method used

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  • New auricularia cornea strain and application thereof
  • New auricularia cornea strain and application thereof
  • New auricularia cornea strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The fungus strain Auricularia cornea of ​​the present invention belongs to Basidiomycotina, Basidiomycetes, Auricularia, Auriculariaceae, Auricularia genus, and horny Auricularia species. Its preservation number is CGMCC NO.23247. Center, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is September 6, 2021.

[0026] The above-mentioned strains were obtained by the applicant in April 2013 by collecting wild fungus fruiting bodies from the wild through separation, purification and qualitative domestication; the specific methods are as follows:

[0027] (1) Obtain the female parent strain Ac7-4 by separating and purifying the wild fungus collected;

[0028] (2) Select the strains with thicker, elastic, taupe, larger flower shape, and veined fruiting bodies from the female parent strains for tissue isolation and purification, and obtain the first generation of tissue isolates after domestication entity, marked F 1 generatio...

Embodiment 2

[0042] This embodiment mainly studies the appearance and antagonism experiment of bacterial strain Guimao No. 7:

[0043] The biggest difference between this strain and the existing Auricularia strains is that there is a typical grid pattern on the back, and its edges are skirt-like folds, which are very unique in appearance, and the ear pieces are large, thick, and rough in texture. Specific as figure 2 and image 3 shown;

[0044] However, the strains currently on the market do not have the above-mentioned appearance characteristics, such as Figure 5-Figure 6 Shown:

[0045] Figure 5 It is the outline drawing of the fruit body of Taimao No. 1, Figure 6 It is the outline drawing of the fruiting body of Auricularia 781.

[0046] And through the antagonism experiment with No. 1 and No. 781 fungus, it can be seen that No. 7 bacterial strain Gui Mao of the present invention has obvious mycelial antagonism with No. 1 and No. 781 bacterial strain, and the strains are not ...

Embodiment 3

[0048] The present embodiment mainly studies the quality of bacterial strain Gui Mao No. 7:

[0049] Adopt the same culture medium to carry out routine cultivation experiment to several Auricularia auricula species:

[0050] ① Fungus varieties: No. 7 Guimao, No. 1 Taimao, No. 781 Fungus

[0051] ② 78 parts of miscellaneous sawdust, 20 parts of wheat bran, 1 part of sugar, and 1 part of gypsum.

[0052] Inoculate 100 original seed bottles and 100 cultivation bags for each variety, measure and calculate the growth rate of mycelium during the strain culture process, and observe and record the mycelium in each original seed bottle and cultivation bag when the strains are full of bottles or bags The results of growth are shown in Table 3.

[0053] Contamination rate (%) = (number of bottles or bags with miscellaneous bacteria / number of inoculated bottles or bags) × 100;

[0054] Yield rate (%)=[(number of inoculated bottles or bags - number of bottles or bags with miscellaneous ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a new auricularia cornea strain and application thereof, the auricularia cornea strain Auricularia cornea Guanghao No.7 is obtained by field collection, separation, purification and domestication from Longhu mountain, Longan County, Nanning, Guangxi province, the strain is particularly unique in form, compared with the existing auricularia cornea strain, the auricularia cornea strain has the following sporocarp forms: the edges of auricularia cornea are slightly bent, the pregnant face is smooth, the back fluff is obvious, the auricularia cornea strain has typical grid lines (veins), and the edge wrinkles are skirt-shaped; further analysis shows that the strain also has the characteristics of high content of crude protein, crude fiber and total amino acid and strong antibacterial ability; meanwhile, according to the formula research of the applicant, a cultivation material formula suitable for the growth of the Guihao No.7 strain is produced by utilizing waste materials.

Description

【Technical field】 [0001] The invention relates to the technical field of microbes, in particular to new strains of cutinous fungus and applications thereof. 【Background technique】 [0002] Horny fungus belongs to Basidiomycotina, Heterobasidiomycetes, Auricularia, Agaricaceae, and Auricularia genus. It is one of the four major edible fungus cultivars in my country. It is named after a large number of fluff on its abdomen and back. In subtropical regions, in warm and humid seasons, it grows on dead branches and dead branches. Compared with black fungus, the ear fruit body is gelatinous, the ear piece is large, thick, rough and tough, hard, brittle and chewy, and has strong stress resistance. Easy to cultivate, shallow disc shape, irregular ears, 2-15μm wide. Obvious base, sessile, base slightly wrinkled, soft when fresh, shrunk when dry. The hymenium grows inside, smooth or slightly wrinkled, purple-gray, and then black. There are longer hairs on the outside, colorless, only...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01H15/00A23L31/00A01G18/20C12R1/645
CPCC12N1/14A01H15/00A23L31/00A01G18/20
Inventor 李发盛罗先群黄雪星何达崇曾维铭欧娜王翠坚李槐黄飞
Owner GUANGXI ACAD OF SCI
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