Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting high performance liquid chromatography

A technology of isopropyl alanine and high-performance liquid chromatography, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the determination method of L-isopropyl alanine hydrochloride that has not been reported yet

Active Publication Date: 2021-10-08
CHONGQING MEDICAL & PHARMA COLLEGE
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no pharmacopoeia standard for related substances of L-alanine isopropyl ester hydrochloride, and the determination method of related substances of L-alanine isopropyl ester hydrochloride has not been reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting high performance liquid chromatography
  • Method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting high performance liquid chromatography
  • Method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The selection of embodiment 1 chromatographic conditions

[0039] 1.1 Selection of wavelength

[0040] Diluent: 25% acetonitrile in water.

[0041] Preparation of L-alanine isopropyl ester hydrochloride solution: Weigh about 10mg of L-alanine isopropyl ester hydrochloride, put it in a 10ml measuring bottle, add diluent to dissolve, dilute to the mark, shake well, Instantly.

[0042] Take L-alanine isopropyl ester hydrochloride solution as the test sample, use the diluent as the blank calibration solution, and scan at 190nm to 400nm according to the ultraviolet-visible spectrophotometry (Chinese Pharmacopoeia 2020 edition). The result shows that need testing solution has maximum absorption peak at 205nm. Therefore, 205nm±2nm was determined as the detection wavelength.

[0043] 1.2 Durability test of the method

[0044] In this test, the chromatographic method of this test is screened by adjusting the mobile phase ratio, flow rate, column temperature, etc. See Table...

Embodiment 2

[0057] Example 2 Specificity

[0058] Chromatographic conditions: the same as in Example 1, scheme 1.

[0059] Solution preparation:

[0060] Blank solution: diluent

[0061] Acid destruction blank solution: Add 2.5ml of 0.1mol / L hydrochloric acid solution to a 5ml measuring bottle, let it stand at room temperature for 4 hours; dilute with 0.1mol / L sodium hydroxide to the mark, shake well, and obtain.

[0062] Acid-breaking test solution: Accurately weigh about 50 mg of the test product, place it in a 5ml measuring bottle, add 2.5ml of 0.1mol / L hydrochloric acid solution, let it stand at room temperature for 4h; dilute to the mark with 0.1mol / L sodium hydroxide, Shake well, and you get it.

[0063] Alkali destruction blank test solution: add 2.5ml of 0.1mol / L sodium hydroxide solution to a 5ml measuring bottle, let it stand at room temperature for 4h; dilute with 0.1mol / L hydrochloric acid to the mark, shake well, and obtain.

[0064] Alkali destruction of the test product...

Embodiment 3

[0076] The stability of embodiment 3 solution

[0077] Chromatographic conditions: the same as in Example 1, Scheme 1.

[0078] Solution preparation:

[0079] Need testing solution: prepare by the same method of 100% concentration level need testing solution in embodiment 1;

[0080] Control solution: accurately pipette 1ml of the test solution, put it in a 100ml measuring bottle, dilute to the mark with diluent, shake well, then precisely pipette 1ml, put it in a 10ml measuring bottle, dilute to the mark with diluent as diluent, shake well , that is.

[0081] Respectively at 0 hour, 12 hours, 24 hours, 48 ​​hours, 10 μl of the test solution and control solution were precisely measured, and the chromatograms at different time points were recorded. The results are shown in Table 4.

[0082] Table 4 solution stability result

[0083]

[0084] The results showed that both the test solution and the control solution were stable within 48 hours.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for determining related substances of L-alanine isopropyl ester hydrochloride by adopting a high performance liquid chromatography. In the process of determining L-alanine isopropyl ester hydrochloride by adopting the high performance liquid chromatography, a chromatographic column taking octadecyl bonded silica gel as a filling agent is adopted, the related substances comprise impurities, namely L-alanine methyl ester hydrochloride and L-alanine ethyl ester hydrochloride, and sodium heptanesulfonate is used as an ion pair reagent. According to the invention, the analysis method is short in analysis time, good in peak shape symmetry, high in recovery rate and high in sensitivity; and by adopting the method, the related substances of the L-alanine isopropyl ester hydrochloride can be rapidly separated and detected, and the content of impurities (L-alanine methyl ester and L-alanine ethyl ester) in the L-alanine isopropyl ester hydrochloride can be accurately determined.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a high-performance liquid chromatography analysis method for determining related substances of L-alanine isopropyl ester hydrochloride. Background technique [0002] L-alanine isopropyl ester hydrochloride (see figure 1 Left) As an important starting material, it is widely used in the synthesis of drugs, such as tenofovir alafenamide fumarate (TAF) (see figure 1 Right) and so on. L-alanine methyl ester hydrochloride and L-alanine ethyl ester hydrochloride in L-alanine isopropyl ester hydrochloride can cause TAF impurities, and the content of related substances needs to be strictly controlled. [0003] In recent years, the requirements for the control of related substances of APIs have become more and more stringent, not only the requirements for limits, but also the requirements for accurate determination of their content. At present, there is no pharmacopoeia standard for related su...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679Y02P20/55
Inventor 邱妍川余虹王韵蒋琳
Owner CHONGQING MEDICAL & PHARMA COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com