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Composite nanofiber membrane with biological responsiveness and preparation method and application thereof

A composite nanofiber, responsive technology, applied in fiber processing, fiber chemical characteristics, drug delivery, etc., can solve the problems of limited application, insoluble, etc., to achieve small side effects, soft touch, and good cell compatibility. Effect

Active Publication Date: 2021-09-07
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are strong hydrogen bonds between chitin molecules and molecules, and it is difficult to dissolve in water, dilute acids, dilute alkalis and conventional organic solvents, which greatly limits its application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] The present invention also provides a method for preparing the composite nanofiber membrane, comprising the following steps:

[0034] 1) After mixing the quaternized chitin, potassium hydroxide, urea and water, sequentially freeze and thaw to obtain the quaternized chitin solution;

[0035] 2) mixing silk fibroin with a solvent to obtain a silk fibroin solution;

[0036] 3) performing a cross-linking reaction with the quaternized chitin solution, the silk fibroin solution, the cross-linking agent and the antibacterial agent to obtain the spinning stock solution;

[0037] 4) Electrospinning the spinning dope to obtain a composite nanofiber membrane.

[0038] The mass ratio of the quaternized chitin, potassium hydroxide, urea and water in step 1) of the present invention is preferably 1:4~6:1~3:60~70, more preferably 1:4.5~5.5:1.5~ 2.5:62~68, more preferably 1:5:2:63~65; the freezing temperature is preferably -25~-30°C, more preferably -27~-29°C, more preferably -28°C; ...

Embodiment 1

[0045] Mix 40g of quaternized chitin, 160g of potassium hydroxide, 40g of urea and 2400g of water to obtain a homogeneous solution, freeze the homogeneous solution at -25°C for 3 hours, and then thaw it under stirring conditions to obtain a quaternized chitin solution . 65g of silk fibroin and 530mL of N,N-dimethylacetamide were mixed at 42°C for 3h to obtain a silk fibroin solution.

[0046]The quaternized chitin solution, the silk fibroin solution, 5g of glutaraldehyde, and 1g of sodium diacetate were subjected to a crosslinking reaction at 52° C., and the crosslinking reaction time was 3.5 hours to obtain a spinning stock solution. Electrospinning was carried out on the spinning dope, the electrospinning voltage was 12kV, the spinning distance was 11cm, and the spinning rate was 2.5mL / h to obtain a composite nanofiber membrane with a porosity of 72% and a fiber diameter of 155nm.

[0047] Performance Test of Composite Nanofiber Membrane

[0048] The composite nanofibrous ...

Embodiment 2

[0056] Mix 50g of quaternized chitin, 280g of potassium hydroxide, 140g of urea and 3000g of water to obtain a homogeneous solution, freeze the homogeneous solution at -30°C for 1 hour, and then thaw it under stirring conditions to obtain a quaternized chitin solution . 85g of silk fibroin and 680mL of trifluoroethanol were mixed at 53°C for 1.5h to obtain a silk fibroin solution.

[0057] Quaternized chitin solution, silk fibroin solution, 7g pentaerythritol, 3g nano TiO 2 The cross-linking reaction was carried out at 68° C. for 1.5 hours to obtain the spinning dope. Electrospinning was carried out on the spinning dope, the electrospinning voltage was 18kV, the spinning distance was 15cm, and the spinning rate was 3.7mL / h, and a composite nanofiber membrane with a porosity of 80% and a fiber diameter of 195nm was obtained.

[0058] Adopt the composite nanofiber film of embodiment 2 to carry out the antibacterial test of staphylococcus aureus and escherichia coli identical w...

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Abstract

The invention belongs to the technical field of composite fiber membranes, and provides a composite nanofiber membrane with biological responsiveness. The composite nanofiber membrane is prepared from the following raw materials in parts by mass: 35-55 parts of quaternized chitin, 60-90 parts of silk fibroin, 4-8 parts of a cross-linking agent, and 0.5-5 parts of an antibacterial agent. The invention also provides a preparation method and application of the composite nanofiber membrane. The composite nanofiber membrane has long-acting and remarkable anti-inflammatory and antibacterial effects, and the inhibition rates of staphylococcus aureus and escherichia coli are 98.5% or above; and the composite nanofiber membrane can promote rapid healing of wounds, is especially suitable for wounds of digestive systems, shows different degradability in different digestive juice environments, and has good biological responsiveness to digestive juice.

Description

technical field [0001] The invention relates to the technical field of composite fiber membranes, in particular to a bioresponsive composite nanofiber membrane and its preparation method and application. Background technique [0002] Biliary tract injury is a rare but harmful complication of liver, gallbladder, and pancreatic surgery, and bile leakage is just one of the manifestations of biliary tract injury. The treatment of bile leakage is varied and complicated, so how to effectively prevent the occurrence of bile leakage is very important. At present, there are studies on the use of fibrin glue on bile duct wounds to prevent postoperative bile leakage, but fibrin glue cannot resist the corrosion of bile, and whether it can prevent postoperative bile leakage is still controversial. [0003] With the development of materials science and tissue engineering technology, more and more biodegradable biomaterials with good biocompatibility have been applied in medical fields su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/728D01D5/00D01F4/00D01F1/10A61L27/20A61L27/22A61L27/50A61L27/54A61L27/56
CPCD01F4/00D01F1/103D01D5/0015D04H1/728A61L27/54A61L27/50A61L27/56A61L27/227A61L27/20A61L2400/12A61L2300/404A61L2300/41A61L2300/602A61L2300/102A61L2300/208A61L2300/21A61L2300/45A61L2300/252A61L2300/232C08L89/00C08L5/08Y02A50/30
Inventor 王彦峰张诗辰杨伊文卞晓恩夏天陈祖涵段博
Owner WUHAN UNIV
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