Bifidobacterium lactis BL-99 capable of resisting oxidation and regulating blood pressure and application thereof

A bifidobacterium lactis, anti-oxidation technology, applied in the direction of bifidobacteria, application, bacteria used in food preparation, etc.

Active Publication Date: 2021-09-07
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]Since the 1990s, antihypertensive research on microorganisms, especially lactic acid bacteria, has been highly valued. It has been reported in relevant literature that lactic acid bacteria can reduce serum cholesterol levels and increase serum cholesterol levels. Medium SOD activity, lowering the level of lipid peroxide, has

Method used

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  • Bifidobacterium lactis BL-99 capable of resisting oxidation and regulating blood pressure and application thereof
  • Bifidobacterium lactis BL-99 capable of resisting oxidation and regulating blood pressure and application thereof
  • Bifidobacterium lactis BL-99 capable of resisting oxidation and regulating blood pressure and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Determination of Growth Kinetic Characteristics of Bifidobacterium lactis BL-99 Fermented Milk

[0032] 1. Experimental strains

[0033] Bifidobacterium lactis BL-99 (CGMCC No.15650) used in the technical solution of the present invention;

[0034] Control strain 1: commercial Bifidobacterium lactis strain BB-12.

[0035] 2. Activation of the strain and culture of seed solution

[0036] (1) Activation of strains: Bifidobacterium lactis was activated with BSM medium, and the strains that had grown to the logarithmic phase were taken and stored in glycerol tubes for future use. Among them, BSM medium (g / L): peptone 5.0g, beef extract 5.0g, tryptone 10.0g, yeast extract powder 5.0g, 0.5% fructooligosaccharides, glucose 10.0g, Tween-80 1.0mL, K 2 HPO 4 2.0g, sodium acetate 5.0g, diammonium hydrogen citrate 2.0g, ZnSO 4 ·7H 2 O 0.25g, MgSO 4 ·7H 2 O 0.1g, 1.0% CaCO 3 (Endogenous adjustment method of pH) Add distilled water to 1000mL.

[0037] (2) Prepar...

Embodiment 2

[0066] Example 2. Fermentation of Bifidobacterium lactis BL-99 to prepare a milk drink with the potential to resist oxidation, regulate blood pressure, and regulate sleep

[0067] This example provides a milk beverage prepared by fermentation of Bifidobacterium lactis BL-99, which has the potential of anti-oxidation, regulating blood pressure and regulating sleep. The manufacturing steps of this drink are as follows:

[0068] (a) inoculating Bifidobacterium lactis BL-99 in skim milk for fermentation, and obtaining fermented milk after fermentation;

[0069] (b) Blending a sweetener and / or a sour agent to form a blended liquid, mixing the blended liquid with the fermented milk prepared in step (a), and homogenizing to prepare a fermented milk beverage.

[0070] Further, the fermentation temperature in the step (a) is 37° C., and the fermentation time is 72 hours.

[0071] Further, in step (b), the mixing volume ratio of the fermented milk and the blending solution is 1:4.

...

Embodiment 3

[0075] Example 3. Bifidobacterium lactis BL-99 fermented to prepare fermented milk with anti-oxidation, blood pressure regulation and sleep regulation potential

[0076] Heat skim milk to above 50°C, add 6% white sugar and stir until completely dissolved, preheat to 60-65°C, homogenize with 20Mpa pressure, heat treat at 90°C for 5-8 minutes, cool to 37°C, inoculate milk double For the seed solution of Mycobacterium BL-99, the inoculum amount is 2% to 3%. Ferment at 37°C for 6-8 hours to make functional fermented milk products that can be directly consumed. The product not only has a sweet and sour taste and a good flavor, but also has the functions of anti-oxidation, regulating blood pressure and regulating sleep.

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Abstract

The invention provides bifidobacterium lactis BL-99 for resisting oxidation and regulating blood pressure and application thereof. The invention firstly provides the application of the bifidobacterium lactis BL-99 in preparation of a composition with the effects of resisting oxidation, regulating blood pressure and/or regulating sleep, wherein the preservation number of the Bifidobacterium lactis BL-99 is CGMCC No.15650. The research discovers that a bifidobacterium lactis strain fermented emulsion has the application in the aspects of oxidation resistance, blood pressure regulation, sleep regulation and the like, and can be widely applied to the fields of foods, medicines, feeds, chemical engineering and the like.

Description

technical field [0001] The present invention relates to the technical field of microbial fermentation, in particular to a kind of bifidobacterium lactis (Bifidobacterium lactis) BL-99 (preservation number CGMCC No. 15650) fermented to prepare a composition with antioxidant, blood pressure and / or sleep regulation effects related applications. Background technique [0002] Aging is the biggest killer of human organs, and free radicals produced by the body are the main culprits of organ aging. When the body's mechanism for regulating free radicals is lost or unbalanced, excessive free radicals will damage cell DNA, change the protein structure in cells, attack cell membranes, and eventually lead to the death of somatic cells. Skin aging is also caused by prolonged exposure to ultraviolet light (UV exposure) or heat source (heat exposure), which induces a sharp increase in the amount of active oxygen, leading to dull skin, skin cancer and other diseases. [0003] Hypertension,...

Claims

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Application Information

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IPC IPC(8): A61K35/745A61P39/06A61P25/20A61P9/12A61K8/99A61Q19/00A23C9/12A23C9/13A23C9/16A23K10/12A23K10/20
CPCA61K35/745A61P39/06A61P25/20A61P9/12A61K8/99A61Q19/00A23C9/1203A23C9/16A23C9/1307A23K10/12A23K10/20
Inventor 赵雯洪维鍊刘伟贤侯保朝尹小静庞飞孙婷
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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