Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A kind of Bacillus cereus and its application

A technology of Bacillus cereus and RBC-1, which is applied in the field of microorganisms, can solve the problems of waste of dilution water, influence on the metabolic capacity of strains, and microbial poisoning, and achieve the effect of improving the effect.

Active Publication Date: 2022-07-15
山东龙泽盛通环保工程有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most important link is to degrade the organic pollutants in the sewage through the microorganisms in the activated sludge, but in general, the high-concentration chlorine-containing wastewater has a toxic effect on the microorganisms, which is mainly manifested in the high osmotic pressure of the wastewater that destroys the microorganisms. Cell membrane and intramembrane enzymes, the metabolic ability of its strains is affected
Therefore, propylene oxide wastewater must be diluted before treatment to reduce the concentration of chloride ions before wastewater treatment can be performed, resulting in a large waste of dilution water

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Bacillus cereus and its application
  • A kind of Bacillus cereus and its application
  • A kind of Bacillus cereus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The screening of Bacillus cereus RBC-1 includes the following steps:

[0031] The strains were screened from the residual sludge of propylene oxide saponification wastewater, and 1 mL of the residual sludge sample was taken, placed in a 150-mL sterile conical flask, 50 mL of sterile saline was added, and 2 g of sterile glass beads were shaken at 25 ° C and 180 rpm for 15 Minutes, suck the bacterial solution, make gradient dilution with sterile water, and dilute to 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 times, draw 100 μL of diluent and apply it to sterile LB solid medium plates containing 5%, 8%, and 10% of sodium chloride, and culture them at 25°C for 2 days. Large colonies were cloned into 50 mL of LB liquid medium containing 10% sodium chloride by mass, cultured at 25°C and 180 rpm for 2 days, and then 100 μL of bacterial liquid was drawn and applied to sterile 10% sodium chloride. LB solid medium to pick single clones.

[0032] The monoclonal bacterial lawn was...

Embodiment 2

[0041] The fermentation culture method of Bacillus cereus RBC-1 comprises the following steps:

[0042]Bacillus cereus RBC-1 was streaked on the LB solid medium, and the activated strain was obtained by inverting activation at 25°C for 1 day; the obtained activated strain was inoculated into the LB liquid medium at 25°C and 200rpm. Shake for 12 hours to obtain seed liquid; take the obtained seed liquid, transfer 1% by volume to LB liquid medium, and expand and cultivate at 25°C and 200 rpm for 12 hours to obtain bacterial liquid; after testing, the bacterial liquid is The bacterial concentration is 2 × 10 8 cfu / mL.

[0043] The liquid medium is LB liquid medium, and the composition is as follows: peptone 10g, yeast powder 5g, sodium chloride 10g, the volume is adjusted to 1L, and the pH is natural.

[0044] The solid medium is LB solid medium, and the composition is as follows: peptone 10g, yeast powder 5g, sodium chloride 10g, agar powder 20g, the volume is adjusted to 1L, ...

experiment example

[0046] (1) Growth of Bacillus cereus RBC-1 under different salinity conditions

[0047] Take 0.1 mL of Bacillus cereus RBC-1 and inoculate it into LB medium, cultivate at 25°C and 200 rpm for 24 hours to obtain the activated bacterial solution; take the prepared activated bacterial solution and inoculate it to different salinities according to 1% by volume In LB liquid medium, salinity (w / v) is 1%, 3%, 5%, 7%, 9%, 11% and 15%, 25°C, 200rpm shaker culture, sampling once every 6h, and the bacteria are measured. Liquid OD 600 , draw the strain growth curve, the results are as follows image 3 shown.

[0048] The liquid medium is LB liquid medium, and the composition is as follows: peptone 10g, yeast powder 5g, sodium chloride 10g, the volume is adjusted to 1L, and the pH is natural.

[0049] Different salinity LB liquid medium, the composition is: peptone 10g, yeast powder 5g, the amount of sodium chloride is calculated according to the salinity (salinity (w / v) is 1%, 3%, 5%, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a kind of Bacillus cereus RBC-1, which was preserved in the China Center for Type Culture Collection on January 7, 2021, and the preservation address is: Wuhan University, Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province , deposit number CCTCC NO: M 2021024; the Bacillus cereus RBC-1 involved in the present invention has the characteristics of high salt resistance, high chlorine, alkalinity and the like, and can withstand 9% salinity and pH=10 environment; inoculation wax The propylene oxide saponified sewage activated sludge of Bacillus-like Bacillus RBC-1 has obvious improvement effect on COD removal.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus cereus and its application. Background technique [0002] Bacillus cereus is about 1-1.3 × 3-5 μm in size, is Gram-positive, and is a facultative aerobic microorganism. Spores can be formed in the body, the ends of the bacteria are relatively flat, and most of them are arranged in chains. Bacillus cereus exists widely in soil and other environments, and is widely used because it can produce abundant metabolites such as protease, SOD, amylase and bacteriostatic substances. At the same time, Bacillus cereus can also be used as an intestinal probiotic to maintain the microecological balance in the intestinal tract, reduce the inflammatory response to protect the intestinal barrier, reduce the incidence of diseases, and improve the body's immunity and disease resistance. At the same time, because of its good resistance to stress and the ability to degr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/085C02F101/34
CPCC12N1/20C02F3/34C02F2101/34
Inventor 王清
Owner 山东龙泽盛通环保工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com