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Application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater

A technology for denitrifying paracoccus and high-salt wastewater, applied in the field of microorganisms, can solve the problems of inability to play an effective role, difficult to achieve purification effects, and high operating costs, improve denitrification efficiency and operation stability, and improve denitrification efficiency. And the effect of running stability and easy use

Pending Publication Date: 2021-12-24
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When treated by biological methods, high-concentration salts have a high inhibitory effect on microorganisms, which are difficult for ordinary microorganisms to bear, and will be inactivated and unable to play an effective role
If the physical and chemical method is used to treat these high-salt wastewater, not only the investment is large, the operating cost is high, but also it is difficult to achieve the expected purification effect

Method used

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  • Application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater
  • Application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater
  • Application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Screening, isolation and identification of Paracoccus denitrificans GBW-HB1904

[0044] 1. Screening and purification of Paracoccus denitrificans GBW-HB1904

[0045] Take 5g of landfill leachate and 5g of pickled food wastewater from food factories, add them to 100mL of PBS buffer solution, shake for 30min to mix the samples thoroughly, centrifuge at 1000rpm for 2min, collect the supernatant of the samples, and set aside. Take 5mL of the supernatant of the above samples and add them to Erlenmeyer flasks containing 100mL high-salinity nitrate nitrogen degrading bacteria enrichment and screening liquid medium, culture at 30°C, 160rpm constant temperature shaker for 2 days, and enrich for 3 times. After gradient dilution of the cultured bacterial suspension, 100 μL was spread on the high-salinity nitrate nitrogen degrading bacteria enrichment and screening solid medium, and placed in a 30°C incubator for cultivation. After 48 hours, single colonies of different...

Embodiment 2

[0050] Example 2: Growth determination and physiological and biochemical characteristics of Paracoccus denitrificans GBW-HB1904

[0051] 1. Growth determination of Paracoccus denitrificans GBW-HB1904

[0052] Inoculate the denitrifying Paracoccus GBW-HB1904 cultured on the slant into the improved NB medium, and culture it on a constant temperature shaker at pH 7.0-7.5 at 28-32°C for 26 hours to prepare GBW-HB1904 bacterial liquid, and samples were taken every 1.0 h Once, the absorbance was measured at OD600 nm, and the growth curve was drawn. Such as figure 2 As shown, the experimental results show that GBW-HB1904 is in the growth retardation phase in the first 5 h of culture, and then enters the logarithmic growth phase, and enters the logarithmic growth phase after 18-20 h of culture, and the number of bacteria reaches 1.0×10 10 CFU / mL, 20~24h is the stable growth period, and then enters the decline period, thus completing the entire growth cycle.

[0053] 2. Physiologic...

Embodiment 3

[0059] Example 3: Salinity Tolerance Test of Paracoccus denitrificans GBW-HB1904

[0060] In order to test the survival performance of Paracoccus denitrificans GBW-HB1904 under high salinity conditions, its growth under different salinity conditions was analyzed and detected. Adjust the amount of sodium chloride in the NB medium to prepare medium with different salt concentrations, and then inoculate the slant-cultured Paracoccus denitrificans GBW-HB1904 into the NB medium with different salt concentrations, at a pH of 7.0~7.5, The culture medium of Paracoccus denitrificans GBW-HB1904 was obtained by culturing in a constant temperature shaker at 30°C for 20 hours, and the growth status of Paracoccus denitrificans in culture medium with different salt concentrations was detected. The results are shown in Table 2. GBW-HB1904 has eurosalinity and can grow well in the salinity range of 25‰~45‰.

[0061] Table 2 Growth of Paracoccus denitrificans GBW-HB1904 in different salt conce...

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Abstract

The invention provides application of paracoccus denitrificans in removal of nitrate nitrogen in high-salinity wastewater. The paracoccus denitrificans is paracoccus denitrificans GBW-HB1904 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.20363, and the paracoccus denitrificans GBW-HB1904 has high salt resistance and can normally grow under the salt concentration of 2.5% to 4.5%. Paracoccus denitrificans GBW-HB1904 fermentation liquor prepared by utilizing the strain can be independently used or compounded with a carrier to form a microbial agent for use, the removal rate of nitrate nitrogen in high-salinity wastewater is higher than 95%, and the biological treatment effect of nitrate nitrogen in high-salinity water can be effectively improved. Meanwhile, the denitrification efficiency and the operation stability of a sewage treatment facility under a high-salt extreme environment condition can be further improved, and the method has a wide application prospect and value.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of denitrifying paracoccus in removing nitrate nitrogen in high-salt wastewater. Background technique [0002] High-salt wastewater generally refers to wastewater with a total salt content of at least 1%, which mainly comes from chemical plants, food processing plants, landfill leachate, and oil and natural gas collection and processing. This kind of wastewater usually contains a variety of biological inhibitory substances, mainly including salt, heavy metals, oil and radioactive substances. With the development of industry, the ways of generating high-salt wastewater are also widely increasing, and its discharge volume is also increasing year by year. Therefore, the removal of organic pollutants in high-salt sewage is of great importance to the environment. When treated by biological methods, high-concentration salts have a high inhibitory effect o...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/28C12N1/20C02F101/16C02F101/38C12R1/01
CPCC02F3/34C02F3/28C12N1/20C02F2101/16C02F2101/38
Inventor 袁绍辉张兆斌孙惠春孙志刚毛赛亚
Owner QINGDAO SHANGDE BIOTECH
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