Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Silicon quantum dots for detecting chlorogenic acid and method for detecting chlorogenic acid

A technology of silicon quantum dots and detection method, applied in the field of nanomaterials, can solve the problems of long sample preprocessing time, long detection time, low sensitivity, etc., and achieve the effects of fast detection speed, low cost and broad application prospects.

Active Publication Date: 2022-06-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Methods for detecting chlorogenic acid include chromatographic analysis, colorimetry, electrochemical methods, etc. However, these methods have certain shortcomings, such as long sample pretreatment time, expensive reagents, long detection time, and low sensitivity. high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Silicon quantum dots for detecting chlorogenic acid and method for detecting chlorogenic acid
  • Silicon quantum dots for detecting chlorogenic acid and method for detecting chlorogenic acid
  • Silicon quantum dots for detecting chlorogenic acid and method for detecting chlorogenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A silicon quantum dot for detecting chlorogenic acid of the present embodiment is prepared through the following steps:

[0047] 1) Add 1 ml APTES (3-aminopropyltriethoxysilane) to 10 ml ultrapure water to obtain A solution;

[0048] 2) Dissolve 0.2 g of o-phenylenediamine in 10 ml of ethanol to obtain solution B;

[0049] 3) Add solution A, solution B and 0.2g PEI (polyethyleneimine) into a 100ml PTFE reaction kettle, and heat to 200°C for 8h reaction;

[0050] 4) After the reaction, cooled to room temperature, the obtained product was dialyzed with a dialysis bag with a molecular weight cut-off of 1000 Da for 12 hours to remove unreacted small molecules, and then freeze-dried to obtain a solid product that is silicon quantum dots.

[0051] refer to figure 1 , figure 1 A is the absorption spectrum and fluorescence spectrum of the silicon quantum dots prepared in Example 1. It can be seen that the maximum excitation light wavelength of the silicon quantum dots is at ...

Embodiment 2

[0053] This embodiment provides a method for detecting chlorogenic acid, which uses the silicon quantum dots prepared in Example 1 for detection, and the specific detection steps include:

[0054] 1) Construct a standard curve for chlorogenic acid detection:

[0055] Mix n parts of the chlorogenic acid solution with a certain concentration gradient with a volume of v1 and a silicon quantum dot solution with a volume of v2 and a concentration of c, respectively, and let stand;

[0056] The obtained n parts of the mixed solution were respectively placed at the excitation wavelength of 350 nm to test the fluorescence intensity value of the mixed solution;

[0057] Taking the chlorogenic acid concentration as the abscissa and the fluorescence intensity as the ordinate, the standard curve for chlorogenic acid detection was obtained by fitting;

[0058] 2) Detect the concentration of chlorogenic acid in the solution to be tested:

[0059] Mix the solution to be tested with a volum...

Embodiment 3

[0073] In this embodiment, the interference experiment of silicon quantum dots used for the detection of chlorogenic acid concentration is carried out.

[0074] In this embodiment, the interfering substances used include Na + , K + , Ca 2+ , Pb 2+ , Ba 2+ , Zn 2+ , Cd 2+ , Al 3+ ,Mn 2+ , CO 3 2- , HCO 3 - , NO 2- , NO 3 - , CH 3 COO - , F - , Cl - , Br - , I - , SO 4 2- , SO 3 2- , PO 4 2- , ClO - ; The concentration is 500μmol / L. The experimental method is: mix the silicon quantum dots with the interferent solution (such as image 3 SiQDs+Interference in the image 3 (SiQDs+Interference+CGA) as a control, CGA is chlorogenic acid, and let it stand for 3 minutes; then at the excitation wavelength of 350 nm, the fluorescence intensity values ​​of the two mixed solutions were tested; the above experiments were carried out for all the interfering substances. refer to image 3 , for the experimental results, it can be seen that in the presence of the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a silicon quantum dot for detecting chlorogenic acid and a method for detecting chlorogenic acid. The silicon quantum dot is prepared through the following steps: 1) APTES is added to ultrapure water to obtain A solution; 2) Dissolve o-phenylenediamine in ethanol to obtain B solution; 3) Mix A solution, B solution and PEI into the reaction vessel and heat for reaction; 4) After the reaction is completed, cool to room temperature and obtain the product by dialysis The bags were dialyzed and then freeze-dried to yield silicon quantum dots. The silicon quantum dots prepared by the invention have the characteristics of fluorescence quenching for chlorogenic acid, and also have the advantages of good stability and high luminous intensity; the detection method of chlorogenic acid established by the silicon quantum dots of the invention has high efficiency, sensitivity and specificity It has strong characteristics, can provide sensitive and accurate detection results, and has broad application prospects in the field of specific detection of chlorogenic acid.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a silicon quantum dot for detecting chlorogenic acid and a method for detecting chlorogenic acid. Background technique [0002] Chlorogenic acid (CGA) is an ester compound formed between caffeic acid and quinic acid, which is widely present in various plants. In addition, many medicinal plants such as honeysuckle, Eucommia and other traditional Chinese medicines contain high levels of CGA. As the main biologically active ingredient, CGA has various functions such as antibacterial blood, antiviral blood, blood pressure lowering, antioxidative blood, and free radical scavenging blood. Based on this, the research on the biological activity of CGA has been carried out from many aspects, among which the analysis method of CGA content has attracted much attention. Therefore, the efficient and sensitive detection of chlorogenic acid is of great significance. [0003] Chlorogenic acid det...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/59G01N21/64
CPCC09K11/59G01N21/6428G01N2021/6432G01N2021/6443
Inventor 昝明辉梅茜董文飞柳裕禄安帅吴再辉葛明锋李力
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com