Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Red ginseng homogeneity polysaccharide as well as extraction method and application thereof

An extraction method and technology of red ginseng, which is applied in the fields of medical formula, allergic diseases, organic active ingredients, etc., can solve the problems of low purity, non-identical red ginseng polysaccharide, failure to detect molecular weight and monosaccharide composition, etc., and achieve strong immunity active effect

Active Publication Date: 2021-06-15
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the invention patent disclosed as CN101550197B, named "a method for recovering ginseng polysaccharides from red ginseng alcohol waste species", the red ginseng root was passed through hot water Extraction, ethanol precipitation, protein removal, and freeze-drying to obtain red ginseng polysaccharides; the disadvantages of comparative documents 1 and 2 are: no further separation and purification by column chromatography, the extracted red ginseng polysaccharides are not homogeneous polysaccharides, and the purity is low , its molecular weight and monosaccharide composition could not be detected;
[0007] In "Separation, Purification and Structural Analysis of Red Ginseng Polysaccharides", the red ginseng was boiled and alcoholized to remove protein, and the corresponding polysaccharide fragments were obtained after column chromatography. Its shortcoming is that red ginseng polysaccharide is not a homogeneous polysaccharide, and the biological activity of red ginseng polysaccharide has not been explored

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Red ginseng homogeneity polysaccharide as well as extraction method and application thereof
  • Red ginseng homogeneity polysaccharide as well as extraction method and application thereof
  • Red ginseng homogeneity polysaccharide as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the extraction of red ginseng homogeneous polysaccharide, such as figure 1 shown.

[0063] 1) Using red ginseng as raw material, crudely extracting red ginseng crude polysaccharide;

[0064] 1-1) Water extraction: Grind the raw material red ginseng to obtain red ginseng powder; add 200 parts of red ginseng powder to the hot water extraction reaction cup, then add 2000 parts of distilled water I, stir in a water bath at 80°C for 2 hours, and collect by filtration Extract solution I; add 1600 parts of distilled water II to the residue, place in a water bath at 80°C and stir for 2 hours, collect extract solution II by filtration; combine extract solution I and extract solution II, centrifuge to remove precipitate, and concentrate under reduced pressure on a rotary evaporator. Obtain the water-soluble crude extract of red ginseng;

[0065] 1-2) Ethanol precipitation: take the cooled red ginseng water-soluble crude extract, add 4 times the volume of absolute ...

Embodiment 2

[0078] Example 2: Identification of molecular weight and purity of red ginseng homogeneous polysaccharide RGRP-1b

[0079] 1. Experimental materials

[0080] The red ginseng homogeneous polysaccharide RGRP-1b, NaNO obtained under the conditions in Example 1 3 , NaN 3 , narrow distribution polyethylene glycol (PEG)

[0081] 2. Experimental method

[0082] RGRP-1b was analyzed by HPGPC system (Shimadzu LC20 gel permeation chromatograph; Japan TOSOH company TSKgel GMPWXL aqueous gel chromatographic column) after loading the sample, and recorded the results with a differential detector (Japan Shimadz company, RID-20a) . The mobile phase is 0.1N NaNO 3 +0.06%NaN 3 Aqueous solution, mobile phase flow rate 0.6mL / min, column temperature 35°C. The standard sample is narrow-distribution polyethylene glycol (PEG). The narrow-distribution PEG is used as the standard curve, and the relative calibration method is used. After the test is completed, the parameters of dextran are used f...

Embodiment 3

[0085] Example 3: Detection of monosaccharide composition and infrared absorption of red ginseng homogeneous polysaccharide

[0086] 1. Experimental materials

[0087] The red ginseng homogeneous polysaccharide RGRP-1b, mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, N-acetyl-glucosamine, glucose, N-acetyl-amino Galactose, galactose, xylose, arabinose, fucose reference substance, PMP-methanol, TFA, chloroform, potassium dihydrogen phosphate solution, sodium hydroxide solution, acetonitrile, NaOH, HCl

[0088] 2. Experimental method

[0089]Configuration of reference solution: Take a clean 15ml EP tube, add 8ml sterile water, add fucose, arabinose, galactose, glucose, xylose, mannose, fructose, ribose, galacturonic acid, glucuronic acid in sequence 100mg of each acid, after dissolving, use a volumetric flask to adjust the volume to 10ml, and prepare a 10mg / ml standard solution mother solution. Dilute the above mixed standard master solution by 100 times to pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses red ginseng homogeneity polysaccharide and an extraction method and application thereof. Red ginseng is used as a raw material, and crude red ginseng polysaccharide is extracted; A red ginseng crude polysaccharide extract is extracted by adopting a DEAE Sepharose TM Fast Flow column chromatography technology to obtain red ginseng polysaccharide RGRP-1. A SephacrylTMS-300High Resolution column chromatography technology is adopted to extract the red ginseng polysaccharide RGRP-1 so as to obtain the red ginseng homogeneity polysaccharide RGRP-1, and the application of the red ginseng homogeneity polysaccharide RGRP-1 in the liver cancer immunoregulation anti-tumor aspect is provided.

Description

technical field [0001] The invention relates to the field of homogeneous polysaccharide extraction, in particular to a homogeneous polysaccharide of red ginseng and its extraction method and application. Background technique [0002] Red ginseng is a traditional Chinese medicine with a long history. In recent years, various biological activities of red ginseng have been widely studied, mainly including anti-inflammatory, cardiovascular effects, slowing down of cell aging, anti-oxidation and anti-tumor activities in vitro. Red ginseng is a plant with high medicinal value, which is distributed in the mountainous forest areas of the Changbai Mountains and southeast of the Lesser Khingan Mountains in China. Red ginseng has been used as a medicinal plant for thousands of years in many countries such as China, Korea, and Japan. In addition, it includes various chemical components such as ginsenosides, polyacetylenes, polyphenolic compounds, and polysaccharides, each of which is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/02A61K36/258
CPCC08B37/006C08B37/0003A61K31/715A61P35/00A61P37/02A61K36/258Y02P20/54
Inventor 鲍依稀张旭裕刘子靖蒲有为杨中伟朱俊默
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com