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Arthrobacter capable of degrading n-eicosane and application thereof

A n-eicosane and degradable technology, applied in the field of microbial remediation of organic pollutants, can solve the problems of application limitations, inconsistent application conditions of strains, and poor stability of bacterial flora, so as to avoid high costs, stable performance, and low requirements for experimental conditions Effect

Active Publication Date: 2021-06-11
WUHAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, Xu Jing et al. used Pseudomonas aeruginosa SJTD-2 to degrade long-chain alkanes in petroleum pollutants. Under theoretical conditions, the degradation effect was good, but in practical applications, the applicable conditions of different strains were inconsistent and the bacterial population was stable. Therefore, it is of great significance to screen a strain of alkane-degrading bacteria with practical application value.

Method used

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  • Arthrobacter capable of degrading n-eicosane and application thereof
  • Arthrobacter capable of degrading n-eicosane and application thereof
  • Arthrobacter capable of degrading n-eicosane and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening, isolation and purification and identification of embodiment 1 bacterial strain C20

[0029] One, the screening method of a n-eicosane degrading bacterial strain C20

[0030] 1. Material preparation

[0031] Soil source for strain screening: Taken from the polluted soil of a coking plant in Huangshi City, Hubei Province, the soil samples were sealed with sampling bags, and brought back to the laboratory at 4°C for storage and later use.

[0032] LB medium: peptone 10.0g / L, yeast extract 5.0g / L, NaCl 10.0g / L, pH 7.0-7.2. Add 1.5% agar powder to the solid medium. Autoclave at 121°C for 20 minutes.

[0033] Mineral salt medium (MSM): KH 2 PO 4 338.8mg / L, (NH 4 ) 2 SO 4 234.0mg / L, Na 2 CO 3 100.0mg / L, CaCl 2 3.9mg / L, MgSO 4 ·7H 2 O 59.3mg / L, Na 2 HPO 4 12H 2 O 890.7mg / L, FeSO 4 0.3mg / L, 1.0mL / L, tryptone 500mg / L, trace element solution (FeCl 2 4H 2 O 1500.0mg / L, CoCl 2 ·6H 2 O190.0mg / L, MnSO 4 ·7H 2 O 100.0mg / L; ZnCl 2 70mg / L, NiCl 2...

Embodiment 2

[0047] Example 2 Degradation of n-Eicosane by Bacterial Strain C20 in Inorganic Salt Liquid Medium Using n-Eicosane as the Only Carbon Source

[0048] 1. Degradation and recovery of n-eicosane

[0049] (1) After streaking the strain C20 on the LB plate, pick a single colony and inoculate it in the LB liquid medium, and culture it in a shaker at 30°C and 200rpm for 24h;

[0050] (2) Centrifuge the bacterial solution after 24 hours of cultivation, discard the culture medium, resuspend it with sterile water, centrifuge again, discard the supernatant and resuspend it with sterile water, repeat this process three times; The solution was adjusted to OD with sterile water 600 = 1.0, transferred to 50mL eicosane as the only carbon source of MSM liquid medium for 7 days, 200rpm shaking culture for 7 days; without adding bacterial strain C20 as a control; control single factor variables, explore environmental factors: temperature, initial bottom The most suitable environment for the d...

Embodiment 3

[0065] Example 3 Application of strain C20 in the aerobic pool test device of the actual discharge of coking wastewater A / A / O treatment process containing n-eicosane

[0066] (1) After streaking the strain C20 on the LB plate, pick a single colony and inoculate it in the LB liquid medium, and culture it in a shaker at 30°C and 200rpm for 24h;

[0067](2) Centrifuge the bacterial solution after 24 hours of cultivation, discard the culture medium, resuspend with sterile water, centrifuge again, discard the supernatant and resuspend with sterile water, repeat this process three times; The solution was adjusted to OD with sterile water 600 = 1.0, transferred to the water body after the wastewater in the operating device was resuspended, and the method of stuffy exposure was used to make it grow and adapt. The effluent water sample 7 days before the addition of the bacteria was used as the control group, and the 7 days after the addition of the bacteria suffocated, the effluent wa...

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Abstract

The invention belongs to the technical field of microbial remediation of organic pollutants, and particularly relates to arthrobacter capable of degrading n-eicosane and application thereof. The name of arthrobacter capable of degrading n-eicosane is Arthrobacter sp.C20, the arthrobacter is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2021367. The arthrobacter capable of degrading n-eicosane can degrade n-eicosane through fermentation, is stable in degradation performance, and can effectively degrade n-eicosane in coking wastewater and reduce CODCr (Chemical Oxygen Demand) of a water body. The arthrobacter disclosed by the invention can be used for degrading n-eicosane through fermentation, so that the problems of high cost, complex process, high technical difficulty, serious secondary pollution and the like in a traditional physical and chemical treatment method are avoided, and no harm is caused to human health and public environment. The arthrobacter disclosed by the invention can be stored permanently, and a foundation is laid for future mechanism research and further industrial application.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic pollutants, and in particular relates to an Arthrobacter strain capable of degrading n-eicosane and its application. Background technique [0002] Studies have confirmed that Arthrobacter bacteria can degrade many environmental organic pollutants such as benzene series, polycyclic aromatic hydrocarbons, and N-heterocyclic compounds, which indicates that Arthrobacter bacteria can grow in a disadvantageous environment and can utilize a variety of organic compounds As its nutrients complete metabolism, Arthrobacter bacteria are considered to be excellent strains that can be applied to environmental bioremediation such as soil and water. [0003] N-eicosane is an important component of long-chain alkanes. It has low water solubility and is difficult to volatilize. Once it enters the environment, it is difficult to be eliminated. It is a persistent pollutant. When the wastewat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/32
CPCC12N1/20C02F3/34C02F2101/32
Inventor 潘明阳王黎明于萍罗运柏
Owner WUHAN UNIV
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