Carboxyl-modified functionalized graphene quantum dot as well as preparation method and application thereof

A technology of carboxyl modification and quantum dots, which is applied in the field of biomedical detection, can solve the problems of poor reproducibility, low detection sensitivity, and high cost of detecting troponin, and achieve the effect of simple preparation method, accurate detection effect and high fluorescence intensity

Pending Publication Date: 2021-06-11
武汉格迪泰健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current detection methods for cTnI require large and expensive instruments to complete, and the detection cost of troponin is high, or the detection sensitivity is not high and the reproducibility is poor. Therefore, there is an urgent need for a functional material that can detect cTnI more sensitively. Carboxyl-functionalized graphene quantum dots have good fluorescence effects, which can be combined with antigens that selectively recognize cTnI, so they can be used as potential functional materials for detecting cTnI

Method used

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  • Carboxyl-modified functionalized graphene quantum dot as well as preparation method and application thereof
  • Carboxyl-modified functionalized graphene quantum dot as well as preparation method and application thereof
  • Carboxyl-modified functionalized graphene quantum dot as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The preparation of graphene oxide (GO): in 30mg sulfuric acid, add the graphite of 2g and the sodium nitrate of 0.5g, in ice bath, continuously stir reaction 4h, then slowly add 5g potassium dichromate and stir and mix with reaction system, be warming up to Stir at 25°C for 1 hour, then add 200ml of deionized water, heat up to 30°C, keep warm for 1 hour, then heat up to 90°C, reflux for 2 hours, after the reflux reaction is complete, cool to 25°C, add 25ml of 30 (w / v)% supernatant Hydrogen oxide solution, filtered after stirring, rinsed the filter cake with 5% hydrochloric acid and deionized water respectively, then dried the filter cake to obtain graphene oxide (GO);

[0031] Weigh 30 mg of prepared graphene oxide (GO) and 30 ml of water, mix and stir evenly to obtain a graphene oxide dispersion, then weigh 3 ml of NMP, mix and stir, disperse in an ultrasonic instrument for 10 min, and transfer the dispersion system to a lined polymer. In an autoclave of tetrafluoroeth...

Embodiment 2

[0041] Preparation of graphene oxide (GO): add 2 g of graphite and 1 g of sodium nitrate to 50 mg of sulfuric acid, continuously stir and react in an ice bath for 3 h, then slowly add 10 g of potassium permanganate and stir and mix with the reaction system, and heat up to 20 ℃, keep stirring for 0.5h, then add 200ml of deionized water, heat up to 35°C, keep warm for 0.5h, then heat up to 80°C, reflux for 3h, after the reflux reaction is completed, cool to 25°C, add 20ml of 30(w / v)% Hydrogen peroxide solution, filtered after stirring, rinsed the filter cake with 5% hydrochloric acid and deionized water respectively, then dried the filter cake to obtain graphene oxide (GO);

[0042] Weigh 30 mg of prepared graphene oxide (GO) and 24 ml of water, mix and stir evenly to obtain a graphene oxide dispersion, then weigh 12 ml of DMF, mix and stir, disperse in an ultrasonic instrument for 10 min, and transfer the dispersion system to the liner. In an autoclave of tetrafluoroethylene, h...

Embodiment 3

[0045] Preparation of graphene oxide (GO): add 2 g of graphite and 2 g of sodium nitrate to 70 mg of nitric acid, stir and react continuously in an ice bath for 5 h, then slowly add 15 g of perchloric acid and mix with the reaction system, heat up to 30 °C Keep stirring for 1.5h, then add 200ml deionized water, heat up to 40°C, keep warm for 1.5h, then heat up to 100°C, reflux for 1h, after the reflux reaction is completed, cool to 25°C, add 30ml of 30(w / v)% supernatant Hydrogen oxide solution, filtered after stirring, rinsed the filter cake with 5% hydrochloric acid and deionized water respectively, then dried the filter cake to obtain graphene oxide (GO);

[0046] Weigh 30mg of prepared graphene oxide (GO) and 36ml of water, mix and stir evenly to obtain a graphene oxide dispersion, then weigh 6ml of ammonia water, mix and stir, disperse in an ultrasonic instrument for 10min, and transfer the dispersion system to the inner lining In a polytetrafluoroethylene autoclave, heat ...

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Abstract

The invention provides a preparation method of carboxyl-modified functionalized graphene quantum dots, wherein the preparation method comprises the steps: preparing graphene oxide through an improved Hummer method, treating the prepared graphene oxide with a nitrogen-containing reagent to obtain N-doped graphene quantum dots, and modifying with organic acid to obtain carboxyl-modified graphene quantum dots. The carboxyl-modified functionalized graphene quantum dots prepared by the three-step method is simple in process, good in stability and high in fluorescence intensity, and has a prospect of being applied to detection of cardiac troponin I.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to a carboxyl-modified functionalized graphene quantum dot and its preparation method and application. Background technique [0002] Graphene oxide (GO) is an oxidized derivative of graphene with a two-dimensional carbon honeycomb lattice with multiple hydroxyl, epoxy, and carboxyl groups, strong hydrophilic properties, easy modification, good biocompatibility, and low cell toxicity. Graphene quantum dots (GQDs) prepared using GO as a raw material have good fluorescence properties. As a new type of nanomaterial, quantum dots can be used to fluorescently label biomolecules through non-covalent bonding and covalent bonding, such as electrostatic adsorption, specific Targeted binding, covalent binding is mainly the combination of carboxylic acid groups on the surface of quantum dots with amino groups and sulfhydryl groups in biomolecules, among which the combination of ca...

Claims

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Application Information

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IPC IPC(8): C01B32/194C01B32/184C01B32/198C09K11/65G01N33/68B82Y20/00B82Y40/00
CPCC01B32/194C01B32/184C01B32/198C09K11/65G01N33/6887B82Y20/00B82Y40/00C01B2204/32C01B2204/20
Inventor 刘建刘明星蒋荣荣童航童庆
Owner 武汉格迪泰健康科技有限公司
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