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Probe electrospray ionization and mass spectrometry combined living body analysis system

An electrospray ionization and analysis system technology, which is applied in the direction of material analysis, material analysis, and measurement device by electromagnetic means, can solve the problems of high cost of metal probe ionization, limited space, high temperature heating, etc., and achieves easy judgment. and guarantee, the positioning method is accurate, and the effect of ionization is enhanced.

Pending Publication Date: 2021-05-28
SHIMADZU (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a living body analysis system using probe electrospray ionization and mass spectrometry, which overcomes the high cost of ionization of metal probes in the prior art, the need for high temperature heating, and the space limitations caused by too close to the probe. technical problem

Method used

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  • Probe electrospray ionization and mass spectrometry combined living body analysis system
  • Probe electrospray ionization and mass spectrometry combined living body analysis system
  • Probe electrospray ionization and mass spectrometry combined living body analysis system

Examples

Experimental program
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Effect test

Embodiment 1

[0050]The effectiveness of the biological tissue sample was simulated by crushing cucumber, examining the effectiveness of the vitamin ionization and mass spectrometry of the probe electrospray ionization.

[0051]The water content of cucumber samples is 96% to 98%, which can reflect the analysis capabilities of high water content (advanced) live tissue samples by the live analysis system. More no spray (Figure 4 (a)) and have spray (Figure 4 (b)) The scan ion mass spectrum of cucumber samples under normal mode can be seen that the cucumber sample is only 1 mass spectrum, and the intensity is 500, which is speculated as noise interference. This is because the water content of cucumber is high, and the ionization efficiency of the target compound is very low. By spraying, the ionization efficiency of the sample is improved after the surface of the probe surface is introduced. Comparing cucumber samples (Figure 4 (b)) and solvent blank (Figure 4 (c) The scan ion mass spectrum can be foun...

Embodiment 2

[0053]The effectiveness of the living analysis system was evaluated by the simulated sample after crushing pork.

[0054]The mass content of pork is about 8%, and the water content is 64-68%, and the ingredient is close to the living animal tissue. For pork samples, under no spray conditions (Figure 5 (a)), almost not observed the effective mass spectrometry, consistent with the results of cucumber sample analysis.

[0055]Compare pork samples (Figure 5 (b)) and system gap (Figure 5 (c)) Scan ion mass spectrum in positive mode, M / Z in pork samples is mainly concentrated in the range of 700-1000, and these substances are mainly lipid ingredients.Figure 5 (d) is the MRM chromatogram of amino acid in pork samples, and a variety of amino acids can also be monitored. In summary, analog samples (cucumber and pork) have shown that the living analysis system has the potential for viable analysis.

Embodiment 3

[0057]By monitoring the types and intensity changes of chemicals in vivo locust brain tissue, study the information pathways and metabolic pathways of locusts should have treated with foreign affairs, and is one of the basic scientific issues of locust research, which is important for ultimate control of locusts. The traditional purification, separation, purification and analysis route is used, it is not only difficult to detect those that are prone to oxidation, light and thermal degradation, but also cause most of the chemical information substances. Based on the living analysis system built in this application, the locust brain tissue sample can be obtained in the case where the locust damage is minimal.

[0058]The analysis process of the locust pressure is as follows: With the help of the microscope, the locust right brain area is observed, and then the probe is moved to the target area by three-dimensional micro-mobile system.Figure 6 The scan mass spectrum of the locust brain ti...

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Abstract

The invention relates to a probe electrospray ionization and mass spectrometry combined living body analysis system. The system comprises a probe electrospray ionization (PESI) system, a spray system and a mass spectrometry system; the spray system comprises a solvent tank, an infusion pump, a compressed air or nitrogen tank, an atomizer, a fog drop screener, a spray pipe and a columnar fog atmosphere; the system further comprises a microscopic positioning system. According to the spray system, continuous and stable columnar mist is generated, and efficient spraying and ionization are achieved; meanwhile, the spray pipe is far away from a metal probe, so that the internal space is effectively released, and the system can have an operation space and is convenient to use; the spray system adopts a conventional liquid chromatography pump to pump out the solvent, and a micro-injection pump is not needed, so that the cost is saved; the system is not limited by a used solvent and is wide in application range; the microscopic positioning system can analyze and measure organs of tiny animals of insects, and a positioning method is accurate and reliable.

Description

Technical field[0001]The present invention belongs to the field of living chemical analysis, and in particular, the present invention relates to a vibratic analysis system for probe electrospray ionization and mass spectrometry.Background technique[0002]The spatial distribution characteristics and molecular foundation of the chemical means of reactive animals and tissue changes. Currently, more and more demand for living chemical analysis. For example, the physiological and biochemical characteristics of pre-transplant organs, brain tissue structure, function and awareness activities of molecular mechanism, model organism (nematode, fruit flies, zebrafish, mice, etc.) growth and development of genetic and metabolic molecular basis. Traditional living analysis techniques, such as nuclear magnetic, X-rays, ultrasound, etc. As an imaging analysis technology, it is possible to effectively reactively reacting the morphology of the changes of animals and tissue, but does not give a molecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 李晓东陈振贺曹磊樊昌俊郭彦丽董静
Owner SHIMADZU (CHINA) CO LTD
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