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A kind of covid-19 antigen detection card, its preparation method and application

A COVID-19, antigen detection technology, applied in the field of nanomaterials and nanomedicine, can solve the problems of virus carriers not being identified in time, not suitable for early detection of infection, consuming manpower and time, etc. The effect of wide detection range and delayed measurement time

Active Publication Date: 2022-05-24
厦门奥德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most virus carriers were not identified in time in the early stage of infection
The main reason is that neither nucleic acid testing nor antibody testing can provide fast and accurate COVID-19 testing results in the early stages of virus infection.
Because nucleic acid testing is an extremely labor-intensive and time-consuming testing method, and it is expensive, and has high requirements for testing equipment, environment, and testing personnel, it can only be carried out in a few professional institutions; Weekly detection rate is extremely low, not suitable for early detection of infection

Method used

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  • A kind of covid-19 antigen detection card, its preparation method and application
  • A kind of covid-19 antigen detection card, its preparation method and application
  • A kind of covid-19 antigen detection card, its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0064] The rare earth nanoprobe of this embodiment is a core-shell structure erbium sodium fluoride coated with yttrium sodium fluoride, the particle size is 20nm-30nm, and its composition is:

[0065] NaErF 4 @NaYF 4 , where NaErF 4 is an all-erbium-doped core structure; NaYF 4 is the shell, @ represents NaYF 4 Coated in NaErF 4 surface.

[0066] Further, the rare earth nanoprobe is stable in the ground state, and emits three emission fluorescences with wavelengths ranging from 500-550 nm, 640-680 nm and 1500-1600 nm under the action of an excitation light source of 808 nm. NaErF 4 @NaYF 4 Fluorescence spectra of rare earth nanoprobes excited at 808 nm, such as figure 2 shown. NaErF 4 @NaYF 4 TEM images of rare earth nanoprobes, such as image 3 shown.

[0067] The method for preparing the rare earth nanoprobe of this embodiment includes the following steps:

[0068] Step 1: Synthesize erbium sodium fluoride core structure: In a container, add oleic acid and 1-...

Embodiment 2

[0072] like figure 1 As shown, the COVID-19 antigen test card of this embodiment includes:

[0073] Substrate 1; as the substrate of the COVID-19 antigen detection card;

[0074] The coating film 2; is arranged in the middle of the upper surface of the substrate 1;

[0075] Sample pad 3: overlapped with one end of the upper surface of the coating film 3

[0076] Absorbent paper 4; overlapped and arranged on the other end of the upper surface of the coating film 3;

[0077] Wherein, the sample pad 2 is sprayed with microsphere lines 21, and the microsphere lines 21 are the rare earth nanoprobe-labeled COVID-19 monoclonal antibody 1 provided in Example 1; the coating film 3 is provided with a detection line 31 and the quality control line 32, the detection line 31 is close to the sample pad 2, the detection line 31 and the quality control line 32 are parallel to each other, and the interval distance is 3-5 mm; the detection line 31 is coated with a COVID-19 monoclonal Antibo...

Embodiment 3

[0088] The COVID-19 antigen detection kit of this embodiment includes:

[0089] The COVID-19 antigen detection kit includes antigen extract R1, which is composed of 1% Triton X-100, 150mM sodium chloride, 1% sodium deoxycholate, 0.1% sodium lauryl sulfate, Tris-HCl (pH 7.4).

[0090] COVID-19 antigen test card: use the COVID-19 antigen test card provided in Example 2;

[0091] ID card with calibration curve: The COVID-19 antigen detection card is used to measure the calibrators of different antigen concentrations, with the antigen concentration as the abscissa and the fluorescence signal ratio as the ordinate, draw a standard curve, write and generate the corresponding QR code Information is stored in the ID card. The corresponding two-dimensional code information on the reagent card can be read and the corresponding antigen concentration can be measured by a dry-type fluorescence immunoassay analyzer.

[0092] The method for quantitatively detecting COVID-19 of the COVID-1...

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Abstract

The invention discloses a COVID-19 antigen detection card, its preparation method and application, and belongs to the field of nanomaterials and nanomedicine, wherein the rare earth nanoprobe used is sodium yttrium fluoride coated with sodium erbium fluoride with a core-shell structure , the particle size is 20nm~30nm, and its composition is: NaErF 4 @NaYF 4 , where NaErF 4 It is an all-erbium-doped core structure; NaYF4 is a shell layer, and @ means that NaYF4 is coated on NaErF 4 surface. The nanoprobe of the present invention is a rare earth fluoride nanomaterial, which has the advantages of low background, long luminescent life, strong fluorescent signal, and high signal-to-noise ratio. The labeling is connected to the new crown monoclonal antibody through a covalent bond, and the labeling product is stable and has With the characteristics of high sensitivity, high accuracy, fast and easy detection, etc., it can screen and detect throat swabs, nasal swabs, nasopharyngeal swabs and saliva samples of patients suspected of having a new crown in the early stage, and can quickly and specifically help clinical diagnosis.

Description

technical field [0001] The invention belongs to the fields of nanomaterials and nanomedicine, in particular to a COVID-19 antigen detection card, a preparation method and application thereof. Background technique [0002] Based on the current epidemiological investigation, the incubation period of the new coronavirus is generally 3-7 days, with a maximum of 14 days. The main symptoms of infected persons are fever, fatigue, and dry cough. Upper respiratory symptoms such as nasal congestion and runny nose are rare. About half of the patients developed dyspnea more than a week later, and severe cases rapidly progressed to acute respiratory distress syndrome, septic shock, refractory metabolic acidosis, and coagulation dysfunction. It is worth noting that severe and critically ill patients may have moderate to low fever or even no obvious fever during the course of the disease. [0003] The new coronavirus, also known as severe acute respiratory syndrome (SARS) coronavirus-2,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/577G01N33/558G01N33/58G01N33/533G01N33/531C09K11/02C09K11/85
CPCG01N33/56983G01N33/577G01N33/558G01N33/587G01N33/582G01N33/533G01N33/531C09K11/02C09K11/025C09K11/7773G01N2333/165G01N2469/10
Inventor 张云张肖刘宁宋良
Owner 厦门奥德生物科技有限公司
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