Fermentation medium and application thereof

A fermentation medium and yeast powder technology, applied in fermentation, microorganism-based methods, and adding compounds to stimulate growth, etc., can solve problems such as differences in the physiological properties of molds, solve the viscosity of fermentation broth, increase yield, and ensure stability. Effect

Active Publication Date: 2021-03-26
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, due to the large variety of molds and the significant differences in the physiological properties of different molds, it is difficult to learn from the technical means of adjusting the fermentation process given in the above documents. In the fermentation process of mold

Method used

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  • Fermentation medium and application thereof
  • Fermentation medium and application thereof
  • Fermentation medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 prepares β-carotene

[0059] This embodiment provides a method for preparing β-carotene by using B. trispora. The steps are as follows:

[0060] 1) Slant culture: prepare PDA slant medium (glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; cut potatoes into 1cm cubes, add deionized water to boil for 30min, filter with four layers of gauze after cooling, Take the filtered supernatant and add glucose and agar powder). Take the spore suspensions of B. trispora positive bacteria and B. trispora negative bacteria, spread them on the PDA slant medium respectively, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0061] 2) Seed culture: Shovel positive bacteria and negative bacteria from the slant surface of B. trispora positive bacteria and negative bacteria strains with an inoculation shovel, respectively, and inoculate them into 1000mL triangular flasks containing 150mL seed culture medium respectively. Cultivate for 48 ho...

Embodiment 2

[0067] Embodiment 2 prepares β-carotene

[0068] This example provides a method for preparing β-carotene by using B. trispora. The difference from Example 1 is that the formulation of the fermentation medium is as follows (the content of alanine is increased), and the total nitrogen content remains the same.

[0069] Table 2

[0070]

[0071]

Embodiment 3

[0072] Embodiment 3 prepares β-carotene

[0073] This example provides a method for preparing β-carotene by using B. trispora. The difference from Example 1 is that the formulation of the fermentation medium is as follows (reduced amount of threonine added).

[0074] table 3

[0075] raw material Ratio (g / L) aspartic acid 1.7 threonine 0.5 glutamic acid 4.0 Alanine 0.6 Lysine 1.3 yeast 22 Potassium dihydrogen phosphate 1 glucose 30 magnesium sulfate 0.5 Sunflower seed oil 45

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Abstract

The invention relates to the technical field of fermentation engineering, in particular to a fermentation medium and application thereof. The fermentation medium comprises a nitrogen source; the nitrogen source comprises aspartic acid, threonine, glutamic acid, alanine and lysine; the content of the aspartic acid is at least 1.0 g/L; the content of the threonine is at least 0.4 g/L; the content ofthe glutamic acid is at least 1.9 g/L; the content of the alanine is at least 0.3 g/L; and the content of the lysine is at least 0.8 g/L. According to the invention, the five specific amino acids arecompounded to replace part of natural nitrogen source raw materials in a conventional fermentation medium, so that the nitrogen source utilization rate of the fermentation medium is remarkably improved, the stability of industrial production is effectively ensured, the negative influence of part of amino acids on carotenoid production is avoided, and relatively high carotenoid yield is maintained; and meanwhile, due to the reduction of the use amount of part of natural nitrogen sources, the problem that short peptides in the fermentation solution influence the viscosity of the fermentation solution is solved to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a fermentation medium and application thereof. Background technique [0002] Carotenoids (carotenoids) is a general term for a class of important natural pigments, commonly found in yellow, orange-red or red pigments of animals, higher plants, fungi, and algae, and is the main source of vitamin A in the body. Highly anti-oxidative properties, it has the effects of removing free radicals in the human body, activating immune cells, preventing cancer and coronary heart disease. β-carotene (BC) and lycopene (LYC) are very representative members of the carotenoid family, which can be used as colorants and nutrients, and have been used in health food, medicine and cosmetics industries. [0003] B. trispora is an important microbial strain that can realize the industrial production of β-carotene and lycopene. [0004] CN104531538A discloses a mutant strain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12P5/02C12N1/38C12R1/645
CPCC12P23/00C12P5/007C12N1/38
Inventor 汪志明李翔宇刘洋余超赵洒陆姝欢
Owner CABIO BIOTECH WUHAN CO LTD
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