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Microbiological detection device and microbiological detection method

A microbial detection and microbial technology, applied in the field of microbial detection, can solve the problems of low sensitivity, complex operation and time-consuming, etc., and achieve the effect of improving applicability

Active Publication Date: 2021-02-12
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a microbial detection device and detection method, which can effectively solve the problems of low sensitivity and complicated and time-consuming operation in the existing devices

Method used

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  • Microbiological detection device and microbiological detection method
  • Microbiological detection device and microbiological detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The sensitive element is made of small-porous membrane and macroporous membrane, the small-porous membrane is mesoporous silicon membrane, and the macroporous membrane is anodized aluminum oxide membrane (AAO);

[0048] The preparation method of small-porous mesoporous silicon membrane is as follows: it is prepared by solution growth method, and the specific preparation method is: select 2cm×2cm ITO glass as the growth substrate of mesoporous silicon membrane, and first use 1mol / L NaOH ethanol solution, acetone solution, ethanol solution and deionized water were ultrasonically washed for 15 minutes in sequence, and then the cleaned ITO glass was immersed in 70mL water, 30mL ethanol, 10μL ammonia water, 0.16g hexadecyl trimethyl bromide Ammonium and 80 μL tetraethyl silicate mixed solution, and reacted at 60 ° C for 20 h; after the reaction was completed, dry the ITO glass at 100 ° C overnight; then, the grown mesoporous silicon film Place the HCl ethanol solution of 0.1...

Embodiment 2

[0051] The sensitive element is made of a small-porous membrane of mesoporous silicon membrane and a macroporous membrane of porous silicon nitride membrane (p-SiN);

[0052] The preparation process of the mesoporous silicon membrane of the small pore membrane: it is prepared by the solution growth method. The specific preparation method is: select 2cm×2cm ITO glass as the growth substrate of the mesoporous silicon membrane, and firstly use 1mol / L NaOH to ITO glass Ethanol solution, acetone solution, ethanol solution and deionized water were ultrasonically washed for 15 minutes in sequence, and then the cleaned ITO glass × glass was immersed in 70mL water, 30mL ethanol, 10μL ammonia water, 0.16g cetyltrimethylammonium bromide and 80 μL of tetraethyl silicate, and reacted at 60 ° C for 14 h; after the reaction was completed, the ITO glass was dried at 100 ° C overnight; then, the grown mesoporous silicon film was mixed with ITO The glass is placed in 0.1mol / L HCl ethanol soluti...

Embodiment 3

[0055] The sensitive element is made of mesoporous carbon membrane of small-pore membrane and anodized aluminum membrane of macroporous membrane;

[0056] The preparation method of the mesoporous carbon membrane: the mesoporous carbon membrane is mainly synthesized on a silicon wafer, using phenolic resin as the carbon precursor, and the triblock polymer F127 as the template agent, and is prepared by the solution volatilization self-assembly method. The specific preparation process of the mesoporous carbon membrane is as follows: (1) Place the silicon wafer (20mm×20mm) in 90°C piranhaic acid (the volume ratio of concentrated sulfuric acid and 30% hydrogen peroxide is 2:1) and wash it for 30 minutes (2) take NaOH as catalyst, phenol and formaldehyde as raw material (mass ratio 1:6:10) to synthesize water-soluble phenolic resin solution, and use absolute ethanol as solvent to prepare mass Fraction is 20% phenolic resin solution; (3) spin coating a drop of phenolic resin solution...

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Abstract

The invention discloses a microbiological detection device and a microbiological detection method. The device comprises a cover plate, a substrate I, a substrate II and a bottom plate which are fixedly connected in sequence from top to bottom, and a sample inlet is formed in one end of the cover plate; the substrate I is provided with a microfluid channel I and a microorganism chamber which are communicated with each other, and the microfluid channel I is communicated with the sample inlet; the substrate II is provided with a separation chamber, a microfluid channel II and a reaction chamber which are communicated with one another, a microorganism separation membrane is fixedly connected between the microorganism chamber and the separation chamber, and a microelectrode I is fixedly connected in the reaction chamber; a buffer cavity is formed in the bottom plate, a sensitive element is fixedly connected between the buffer cavity and the reaction cavity, a microelectrode II is fixedly connected in the buffer cavity, a buffer solution inlet and a buffer solution outlet are formed in the two sides of the buffer cavity respectively, and the buffer solution inlet is formed in the lower portion of the reaction cavity. The device can effectively solve the problems that an existing device is low in sensitivity and complex and time-consuming in operation.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a microorganism detection device and a detection method. Background technique [0002] Microorganisms widely exist in nature. Pathogenic microorganisms refer to the general term of microorganisms that can cause infection, also known as pathogens, mainly including viruses, bacteria, fungi, mycoplasma and prions, etc. Among them, viruses and bacteria are the greatest threat to humans. Food and water safety incidents caused by pathogenic bacteria seriously threaten human life and health. 54.3% of sudden infectious diseases in the world are caused by bacteria or Rickettsia. [0003] The microorganism detection method used in the prior art is mainly the microorganism cultivation method. Although there are many microorganism cultivation devices on the market, these devices require a long cultivation time, which is severely restricted in the rapid detection a...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/31G01N27/327G01N27/333G01N27/36B01L3/00G01N33/569
CPCB01L3/502761B01L2200/0668B01L2300/0887G01N27/307G01N27/308G01N27/31G01N27/3278G01N27/3335G01N27/36G01N33/56911G01N2333/21
Inventor 李顺波马文瑞吕自兰徐溢葛闯陈李王力
Owner CHONGQING UNIV
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