Rhizobium pumilum and application of rhizobium pumilum in preparation of water-soluble beta-1,3 glucan

A technology of Pusha root nodule and glucan, applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low extraction rate, cumbersome extraction process, high cost, and achieve the effect of high fermentation yield

Active Publication Date: 2021-02-12
河北沣川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the β-1,3 glucan was purified, but the purification method required fermentation broth→alcohol precipitation→dissolution→sevag method to remove protein 3 times→alcohol precipitation→dissolution→over DEAE cellulose and agarose gel→ Alcohol precipitation→drying→crushing, the existing problems of this prior art are that the fermentation yield is not explained; the second is that the extraction process is complicated, the cost is high, the extraction rate is low, and it is not suitable for industrial production.

Method used

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  • Rhizobium pumilum and application of rhizobium pumilum in preparation of water-soluble beta-1,3 glucan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073]A Rhizobium pusense, its Latin name is Rhizobium pusense, and the deposit number is CGMCC No.19764.

[0074]The application of Rhizobium pusa in preparing water-soluble β-1,3-glucan includes the following process steps:

[0075]A. Inoculate Rhizobium pusense with the preservation number CGMCC No.19764 and the Latin name Rhizobium pusense into the sterilized culture solution containing carbon source, nitrogen source and other necessary nutrients;

[0076]B. Aeration and stirring fermentation at a temperature of 28°C;

[0077]C. When the fermentation period does not exceed 70 hours, the fermentation is finished to produce a fermentation broth containing β-1,3-glucan.

[0078]The carbon source in the culture solution of step A is sucrose; the nitrogen source is selected from one or a combination of soybean protein isolate, ammonia water, ammonium nitrate, a mixture of peptone and yeast powder.

[0079]In the step A, the culture solution contains the following components by mass percentage:

[0080]Su...

Embodiment 2

[0110]The difference between this embodiment and embodiment 1 is:

[0111]The application of Rhizobium pusa in preparing water-soluble β-1,3-glucan includes the following process steps:

[0112]A. Inoculate Rhizobium pusense with the preservation number CGMCC No.19764 and the Latin name Rhizobium pusense into the sterilized culture solution containing carbon source, nitrogen source and other necessary nutrients;

[0113]B. Aeration and stirring fermentation at a temperature of 32℃;

[0114]C. When the fermentation period does not exceed 70 hours, the fermentation is finished to produce a fermentation broth containing β-1,3-glucan.

[0115]In the step A, the culture solution contains the following components by mass percentage:

[0116]Sucrose 6.0%; Soy protein isolate 0.3%; Sodium dihydrogen phosphate 0.2%; Sodium nitrate 0.3%; Magnesium sulfate 0.025%; Ferrous sulfate 0.0015%; Zinc chloride 0.001%; ​​Antifoaming agent 0.05%; pH=7.5.

[0117]Step A is to inoculate Rhizobium pusa with the preservation nu...

Embodiment 3

[0147]The difference between this embodiment and embodiment 1 is:

[0148]The application of Rhizobium pusa in preparing water-soluble β-1,3-glucan includes the following process steps:

[0149]A. Inoculate Rhizobium pusense with the preservation number CGMCC No.19764 and the Latin name Rhizobium pusense into the sterilized culture solution containing carbon source, nitrogen source and other necessary nutrients;

[0150]B. Aeration and stirring fermentation at a temperature of 30℃;

[0151]C. When the fermentation period does not exceed 70 hours, the fermentation is finished to produce a fermentation broth containing β-1,3-glucan.

[0152]In the step A, the culture solution contains the following components by mass percentage:

[0153]Sucrose 5.5%; soy protein isolate 0.22%; sodium dihydrogen phosphate 0.15%; sodium nitrate 0.22%; magnesium sulfate 0.02%; ferrous sulfate 0.0013%; zinc chloride 0.0008%; defoamer 0.04%; pH=7.1.

[0154]Step A is to inoculate Rhizobium pusa with the preservation number of ...

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Abstract

The invention belongs to microbial fermentation, and particularly relates to rhizobium pumilum and an application of the rhizobium pumilum in preparation of water-soluble beta-1,3 glucan. A method comprises the following process steps: inoculating rhizobium pumilum with the preservation number of CGMCC No.19764 into a sterilized culture solution containing a carbon source, a nitrogen source and other necessary nutrient substances, ventilating, stirring and fermenting at the temperature of 28-32 DEG C, and finishing the fermentation when the fermentation period does not exceed 70 hours to prepare fermentation liquor containing beta-1, 3 glucan. The method solves the problems of tedious process, low product yield and the like in the prior art, and the product has the advantages of high yield, high product viscosity, good water solubility, low fermentation cost and the like.

Description

Technical field[0001]The invention belongs to microbial fermentation, and particularly refers to a Pusa rhizobium and its application in preparing water-soluble β-1,3-glucan.Background technique[0002]β-1,3-glucan is a type of glucose polymer with various biological activities connected by β-1,3 glycosidic bonds. It not only exerts a variety of biological activities in the organism, but also interacts between various organisms. It also has a variety of functions during the impact process. It is a highly active biological response regulator with low toxic and side effects, and has unique physiological and pharmacological activities. At present, the biomedical community generally believes that β-1,3-glucan has four major physiological functions, including cleansing the intestines, lowering cholesterol, regulating blood sugar, and improving immunity. Because β-1,3-glucan has the functions of regulating blood sugar, improving immunity, anti-tumor, anti-infection and anti-oxidation, it ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/08C12R1/41
CPCC12N1/20C12P19/08C12R2001/41C12N1/205
Inventor 乞锋辉杨晓民刘学珍脱世华靳晓伟
Owner 河北沣川生物科技有限公司
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