Modification method of gelatin

A modified gelatin technology, applied in the field of biomedical materials, can solve the problems of unsatisfactory biomedical materials, unsatisfactory application scenarios, incompatible chemical reagents, etc., and achieve easy control of reaction parameters, simple modification methods, and degradable strong effect

Inactive Publication Date: 2021-01-08
深圳大洲医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of dissolving gelatin in water under heating conditions is not ideal for certain applications
At the same time, the thermal stability of this physical gel is relatively poor, and it can be dissolved again under heating conditions. Therefore, in order to prepare a more stable gel, gelatin is often physically cross-linked or chemically cross-linked in the existing technology to improve gelatin. Forming gel thermal stability or improving other properties, more chemical cross-linking methods are used. Common gelatin chemical cross-linking agents include formaldehyde, glyoxal, glutaraldehyde, 1,6-hexamethylene diisocyanate, butyl Diene diepoxide, maleic acid, dopamine hydrochloride, etc.
Such chemical crosslinking often involves bioincompatible chemical reagents and does not meet the needs of the field of biomedical materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 10.0 g of gelatin was added to 100 mL of deionized water to a final concentration of 10% (w / v), and placed in a round bottom flask with a magnetic stirring bar. The resulting mixture was stirred moderately in a 50 °C water bath for 30 minutes to facilitate dissolution of the gelatin until the solution became clear when the gelatin was completely dissolved. Then, while stirring, slowly add 1.0 g of methacrylic anhydride (dropping speed: 0.02 mL / min) into the round bottom flask to obtain methacrylate functionalized gelatin, i.e. modified gelatin methacrylic acid ester. Due to the addition of methacrylic anhydride, the solution will become homogeneous and opaque, make sure to stir well during the reaction, while minimizing the absorption of air, because insufficient stirring will lead to phase separation of the solution. After the addition of methacrylic anhydride was complete, the reaction was stirred for 5 hours while using 7 mL of 1M NaHCO 3 The solution adjusted the ...

Embodiment 2

[0056] 10.0 g of gelatin was added to 100 mL of deionized water to a final concentration of 10% (w / v), and placed in a round bottom flask with a magnetic stirring bar. The resulting mixture was stirred moderately in a 50 °C water bath for 30 minutes to facilitate dissolution of the gelatin until the solution became clear when the gelatin was completely dissolved. Then, while vigorously stirring, slowly add 0.6 g of methacrylic anhydride (dropping rate: 0.30 mL / min) into the round bottom flask to obtain methacrylate functionalized gelatin, namely modified gelatin methyl Acrylate. Due to the addition of methacrylic anhydride, the solution will become homogeneous and opaque, make sure to stir well during the reaction while minimizing the absorption of air. After the addition of methacrylic anhydride was complete, the reaction was stirred for 3 hours while using 5 mL of 1M NaHCO 3 The solution adjusted the pH of the reaction solution to 7. After the reaction, transfer the react...

Embodiment 3

[0058] Take 1.0 g of the modified gelatin methacrylate with a medium degree of substitution obtained in Example 1 and dissolve it in 20 mL of deionized water, heat and stir in a water bath at 50° C. for 40 minutes to dissolve the modified gelatin methacrylate to form a uniform modified gelatin methacrylate. Aqueous gelatin methacrylate aqueous solution, prepared as a solution with a mass fraction of 5%; add 0.5mL triethylamine to adjust the pH value of the reaction solution to 8, and dissolve 0.5g succinic anhydride in 10mL dimethyl sulfoxide Add it into the uniform and transparent solution, the dropping rate is 0.1g / min, so that the molar ratio of the modified gelatin methacrylate to succinic anhydride is 1:10, and the reaction temperature is 50°C under magnetic stirring for 18h, with 0.1 The HCl solution of M adjusts the pH value of the reaction solution to neutrality. The obtained crude product was centrifuged at 3500g for 3 minutes, and the supernatant was dialyzed in deio...

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Abstract

The invention provides a modification method of gelatin, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: carrying out chemical modification by introducing crosslinkable functional groups into gelatin to obtain modified gelatin methacrylate, further reacting a second compound containing intramolecular anhydride functional groups withamino groups in the modified gelatin methacrylate, and introducing carboxyl groups to prepare the gelatin derivative material modified by two anhydrides. Gelatin molecules contain a large number of active functional groups such as amino groups and hydroxyl groups, so that reactive functional groups can be introduced and the solubility can be improved through the chemical modification method.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a gelatin modification method. Background technique [0002] Gelatin generally refers to the hydrolyzate of animal collagen. It is a natural polymer material. Due to its excellent biocompatibility and degradability, it has been widely used in many fields in recent years. For gelatin, it has a wide range of sources, is cheap, environmentally friendly, green and non-toxic, and is a natural polymer material with great application value. However, pure gelatin has low thermal stability, poor mechanical properties, and easy hydrolysis. The disadvantage is that it cannot be directly used as a market application material. Taking the abundant functional groups on amino acid residues in gelatin molecules as a breakthrough, chemically modify them to obtain higher solubility and reactivity, so that they can obtain higher application value and broader application prospects . ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00
CPCC08H1/00
Inventor 岳衎姚中麒吴天昊杨景周杨景浩
Owner 深圳大洲医学科技有限公司
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