Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting biomarker of Alzheimer's disease and detection method thereof

A technology of biomarkers and kits, applied in biological testing, biological material analysis, measuring devices, etc., can solve problems such as poor stability, achieve the effects of improving corrosion resistance, reducing usage, and simplifying detection steps

Pending Publication Date: 2021-01-01
NANJING LEADING BIOMEDICAL TECH CO LTD
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention lies in the relatively poor stability of the Alzheimer's disease biomarker kit in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting biomarker of Alzheimer's disease and detection method thereof
  • Kit for detecting biomarker of Alzheimer's disease and detection method thereof
  • Kit for detecting biomarker of Alzheimer's disease and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Kit for detecting biomarkers of Alzheimer's disease, including horseradish peroxidase-labeled biomarker antibody solution, biomarker antibody-coated microtiter plate, concentrated washing solution, luminescent substrate solution, calibration products and quality controls. The biomarker in this example is p-Tau-181 protein.

[0060] (1) Preparation of horseradish peroxidase-labeled p-Tau-181 antibody solution

[0061] (1) Dissolve 0.5mg of horseradish peroxidase (HRP) in 0.25ml of distilled water, then add 0.1ml of NaIO 4 (0.06M / L) solution, after mixing, place it at 4°C in the dark for 30 minutes, the solution is yellow-green;

[0062] (2) Add 0.1ml of ethylene glycol solution, and stir gently for 30min at room temperature in the dark;

[0063] (3) Add 0.5 mg of p-Tau-181 antibody (concentration adjusted to 3 mg / ml), mix well, put it into a dialysis bag (mw=3500-14000), and place it in CB solution (carbonic acid buffer solution with pH=9.5) ) for 6 hours of dialysis...

Embodiment 2

[0104] The difference between this example and Example 1 is that: the biomarker is Aβ1-42 protein, the calibrator in the kit is 0, 75, 150, 300, 600, 1200 pg / ml; the concentration of the quality control substance is 150 pg / ml, 600 pg / ml.

Embodiment 3

[0106] Test kit performance in embodiment 1

[0107] Test the calibration curve, sensitivity, linear range, precision and stability of the kit in Example 1. The specific operation steps are as follows:

[0108] (1) Standard curve: (a) Add the prepared calibrator and quality control product in sequence to the microtiter plate coated with anti-p-Tau-181 antibody. In order to ensure the stability of the experiment, add two wells for each concentration , add 100uL to each well. The microwell reaction plate was sealed with a membrane and incubated at 37°C for 1 h.

[0109] (b) washing plate:

[0110] ① If the plate is washed manually: fill each well with washing solution, let it stand for 10 seconds, shake off the liquid in the well, and pat dry on absorbent paper, repeat this process 3 times.

[0111] ②If using a plate washer to wash the plate, select the program of washing 3 times, and pat dry after washing.

[0112] (c) Enzyme marker: Add 100uL horseradish peroxidase-labele...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting a biomarker of an Alzheimer's disease, and relates to the technical field of in vitro diagnosis. The kit comprises a horse radish peroxidase labeled biomarker antibody solution, an anti-biomarker antibody coated elisa plate, a concentrated washing solution, a luminous substrate solution, a calibration product and a quality control product. The inventionfurther discloses a preparation method of the elisa plate coated with the anti-biomarker antibody. The kit has the beneficial effects that on the basis of chemiluminiscence immunoassay, the elisa plate coated with the anti-biomarker antibody is introduced, a coating process of the anti-biomarker antibody is improved, the reaction sensitivity is increased, a reagent production process is simplified, production time is shortened, and the detection kit which is high in quality, low in price, stable, reliable, good in repeatability, small in batch difference and high in accuracy is provided for the market.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a kit and a detection method for detecting biomarkers of Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease with insidious onset and progressive development. It is a cranial nerve disease first discovered by the German doctor Alzheimer. It has two main symptoms: one is senile plaques formed by the abnormal deposition of β-amyloid protein outside the neuron cells, and the other is NFTs (neurofibrillary tangles) formed by the abnormal phosphorylation of tau protein, which seriously affects the patient's health. Life and social functions seriously affect the quality of life of patients and close relatives. The etiology and pathogenesis of AD are still unclear, and the characteristic pathological changes are extracellular senile plaques formed by the deposition of β-amyloid protein, neurofibrillary tangles formed by...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/535G01N33/543
CPCG01N33/6896G01N33/535G01N33/54393G01N2333/4709G01N2800/2821
Inventor 张玉基叶生宝王鹏王维李叶仲子进
Owner NANJING LEADING BIOMEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com