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Streptococcus suis type 3 vaccine strain and application thereof

A technology of Streptococcus suis and vaccine strains, applied in vaccines, veterinary vaccines, bacteria, etc., can solve the problems of Streptococcus suis being prone to drug resistance, lack of efficient Streptococcus suis type 3 vaccine, etc., to achieve effective resistance to virulent Attack, good growth performance and immunogenicity, effect of excellent immunogenicity

Active Publication Date: 2020-12-11
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, sensitive drugs such as amoxicillin and cephalexin are commonly used for the treatment or preventive use of Streptococcus suis disease, but Streptococcus suis is very prone to drug resistance, and as the country restricts the use of antibiotics in the breeding process more and more strictly, the use of Safe and effective vaccines to prevent and control bacterial diseases such as Streptococcus suis have become a trend and research hotspot
At present, there are commercialized vaccines for serotypes of Streptococcus suis type 2 and 7 in my country, but there is still a lack of highly effective Streptococcus suis type 3 vaccine

Method used

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  • Streptococcus suis type 3 vaccine strain and application thereof
  • Streptococcus suis type 3 vaccine strain and application thereof
  • Streptococcus suis type 3 vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Bacterial Isolation

[0025] The disease material was collected from the lungs of acutely dead pigs from a pig farm in Jiangsu. After aseptically treating the surface of the collected lungs, a small piece of internal tissue was aseptically cut out and placed in THB liquid medium. Pick up the enrichment solution from the inoculation loop and inoculate it on the THB plate, and incubate at 37°C for 24 hours. Pick milky white, translucent, circular suspected colonies with a diameter of 1-2 mm, and then inoculate them in THB liquid medium for enrichment, then draw the enrichment solution on THB plates, and then pick single colonies and culture them on THB plates until a single pure colony. Pure cultures of several strains were finally obtained.

[0026] 2. Identification

[0027] (1) Gram staining identification of isolates

[0028] The pure cultures of each strain were picked, and Gram staining was carried out according to the method of "The Veterinary Pharmacopoeia ...

Embodiment 2

[0059] Embodiment 2 immune protection test

[0060] Preparation of Streptococcus suis type 3 oil emulsion vaccine: Take Streptococcus suis type 3 JSHZ strain out of the -70°C refrigerator, streak on THB solid medium, culture in a 37°C incubator for 24 hours, and pick 3 single colonies In 5mL THB liquid medium, place on a shaker at 37°C, shake the bacteria at a speed of 200rpm overnight, take 4mL of the bacterial liquid from it and add it to 200mL THB liquid medium, place it on a shaker at 37°C, 200rpm, and cultivate 6 Hour. Take a small amount of bacterial liquid to carry out plate counting of viable bacteria, and the result shows that the concentration of bacterial liquid is 2.5×10 9 cfu / mL. Add formaldehyde with a final concentration (volume percentage) of 0.3% to the rest of the bacterial solution, place it in a constant temperature shaker at 37° C. with a rotation speed of 100 rpm for 48 hours for inactivation, and then place it at 4° C. for use. Take 200 μL of inactiva...

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Abstract

The invention provides a streptococcus suis type 3 vaccine strain and application thereof, and relates to the field of veterinary vaccine. The streptococcus suis type 3 vaccine strain is classified and named as streptococcus suis type 3 JSHZ, and the preservation number is CCTCC NO: M 2019687. The invention also provides a vaccine containing the streptococcus suis type 3 vaccine strain and application of the strain in preparation of medicines for treating or preventing streptococcus suis type 3 infection. The streptococcus suis type 3 vaccine strain shows strong pathogenicity to mice and pigs,has good growth performance and immunogenicity, and is an excellent streptococcus suis type 3 vaccine strain. After the vaccine is adopted to immunize piglets, virulent attack can be effectively resisted, and excellent immunogenicity is shown.

Description

technical field [0001] The invention relates to the field of veterinary vaccines, in particular to a Streptococcus suis type 3 vaccine strain and applications thereof. Background technique [0002] Streptococcus suis (abbreviated as Ss) is an important zoonotic pathogen, which can cause diseases such as meningitis, sepsis, arthritis, endocarditis, etc., and can cause a large number of piglets to die. cause significant economic losses. Through capsule typing, Streptococcus suis has 33 serotypes (types 1 to 31, 33 and 1 / 2), among which serotype 2 has the highest isolation rate in sick pigs and people, and serotype 3 (abbreviated as Ss 3), 7 and 9 types are also common in many countries and are strongly associated with S. suis infection and disease in swine herds. Clinically, sensitive drugs such as amoxicillin and cephalexin are commonly used for the treatment or preventive use of Streptococcus suis disease, but Streptococcus suis is very prone to drug resistance, and as the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/09A61P31/04C12R1/46
CPCA61K39/092A61P31/04A61K2039/521A61K2039/552C12R2001/46C12N1/205
Inventor 肖琦何孔旺俞正玉汪伟倪艳秀祝昊丹周俊明王丹丹温立斌
Owner JIANGSU ACAD OF AGRI SCI
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